báo cáo khoa học: " Singlet oxygen mediated DNA degradation by copper nanoparticles: potential towards cytotoxic effect on cancer cells" pot

... of 8 SHOR T COMM U N I C A TIO N Open Access Singlet oxygen mediated DNA degradation by copper nanoparticles: potential towards cytotoxic effect on cancer cells Gregor P Jose 1 , Subhankar Santra 2 , ... induced DNA degradation procee ds through a singlet oxygen mediated mechanism. In order to calculate the rate con- stant of the DNA degradation by...

Ngày tải lên: 11/08/2014, 00:23

... at a nanomolar DNA concentration (data AB C E D Fig. 4. DNA length requirement for DNA unwinding by TIP49b. (A) Schematic presen- tation of the DNA substrates containing 3¢ ssDNA tails of different ... nM) and increasing concentrations of TIP49b monomers. Binding dependence on ssDNA length follows that seen for ATP hydrolysis. (E) Compar- ison between ssDNA and duplex DNA bindin...

Ngày tải lên: 15/03/2014, 11:20

... organization. NAP-22 together with related proteins, function to enhance the accumulation and assembly of PtdIns(4, 5)P2-rich raft domains [36]. During neuronal develop- ment, axonal elongation and ... will affect neuronal growth and development. CAP-23 accumulates in the neuronal growth cone and has a marked effect on the rearrange- ment of the actin cytoskeleton [38]. An early conse- qu...

Ngày tải lên: 07/03/2014, 17:20

... had no significant effect on ERK1 ⁄ 2 activation (Fig. 4B). We also confirmed inhibition of protein synthesis by monitoring expression of c-Fos protein, which is a Fig. 2. Activation of ERK1 ⁄ 2 pathway ... activation of ERK1 ⁄ 2. However, transient activation of ERK ⁄ 2 upon EGF stimulation did not induce miR-221 ⁄ 222 expression. This observation prompted us to hypothesize that inductio...

Ngày tải lên: 16/03/2014, 01:20

... to a concentration of 100 mm, and incubation was continued for an additional 30 min. Sepharose CL-6B The autolysed preparation was cleared by centrifugation and subjected to gel filtration through ... oligodeoxyribonucleo- tide. Reactions were carried out at 65 °C for 15 min and terminated by the addition of 10 lLof10mm Na-EDTA. Nucleic acids were isolated by phenol extraction [53] and a...

Ngày tải lên: 16/03/2014, 06:20

... or nonconventional T cells, may develop via an indepen- dent pathway, dependent on expression of major histo- compatability complex molecules on bone-marrow- derived cells. These nonconventional ... lineage. Unlike conventional T cells, iNKT cells are selected by CD1d expression on immature double-positive thymocytes [15,16]. Efficient selection of iNKT cells also depends on TCR sign...

Ngày tải lên: 28/03/2014, 22:21

... assay conditions using labe- led and exogenously added nonlabeled AA, and demonstrates the crucial alteration of the dose– response curve in the presence of exogenously added AA. This fact demonstrates ... Leukocyte-rich plasma was prepared by Dextran sedimentation. Granulocytes were obtained from leukocyte-rich plasma by centrifugation on Ficoll–Paque and hypotonic lysis of erythrocyte...

Ngày tải lên: 30/03/2014, 11:20

... study, ASP expression was highly expressed during cell differentiation and maturation and confirmed the relation between ASP and TG accumulation; comparable finding was confirmed in another ... 201 activation of protein kinase C, Akt and MAPK/ERK1/2, all of which lead to an effective and prolonged stimulation of TG synthesis [16]. In conclusion, this study showed the stimulatory effec...

Ngày tải lên: 07/08/2014, 23:22

... provenances andprogenies, and pronouncedfor clones. Model 1 Model 1b Model 2 Variation explained by linear model Provenance Family Clone Provenance Family Clone Provenance Family Clone F 763 1314 423.4 ... (selection based on survival and growth). After selection of the best 20% clones in 1992, a thinning was conducted of the 80% clones not selected as superior. During the winter of 1997–...

Ngày tải lên: 08/08/2014, 14:21

... and Sons, Inc New York, 354 p Heinselman ML (1981) Fire and succession in the conifer forests of northern North America. In: Forest succession: Concepts and applica- tion (DC ... from all-sized stands. DISCUSSION The first hypothesis set for this study (ie that mixed (broad leaved-coniferous) stands were better than conifer stands) was con- firmed f...

Ngày tải lên: 08/08/2014, 19:21