báo cáo khoa học: " Nucleoside conjugates of quantum dots for characterization of G protein-coupled receptors: strategies for immobilizing A2A adenosine receptor agonists" pdf

... cited. Research Nucleoside conjugates of quantum dots for characterization of G protein-coupled receptors: strategies for immobilizing A 2A adenosine receptor agonists Arijit Das, Gangadhar J Sanjayan, ... Nucleoside conjugates of quantum dots for characterization of G protein-coupled receptors: strategies for immobilizing A2A ad...

Ngày tải lên: 11/08/2014, 00:22

... H1A fragments Lys152±Lys222 and Lys167±Lys222 were modi®ed by Arg-Gly-Gly at Lys222 via an isopeptide bond between that e-amino group and the a-carboxyl group of the terminal Gly of Arg-Gly-Gly. ... with the c harge residing on t he C-termin us of the peptide. Scheme 2. Sc hematic structure of uH1A fragment. The a-carboxy group of Gly is linked to the e-amino group of Lys222 of...

Ngày tải lên: 08/03/2014, 10:20

... Higgins, D .G. & Gibson, T.J. (1994) CL USTAL W: improving the sensitivity of progressive multiple sequence alignment through sequen ce weighting, positions-sp ecific gap penalties and wei ght ... in large quantities ( 50 mg per litre of culture) in a highly purified form (> 95%). The purified protein gave a single band at  50 kDa on SDS/PAGE. MALDI-TOF analysis gave a mean ± SD mas...

Ngày tải lên: 16/03/2014, 18:20

... 5¢-CACTCTAGAC- CATGGCTGCATCCCCTGAGCTCAGT-3¢ and 5¢-CAC- GGATCCCTACTTGCCCCCTTGCAC-3¢ for P25aDN, and 5¢-CACTCTAGACCATGGCTGACAAGG-3¢ and 5¢-CACGGATCCCTACGTCACCCCTGA-3¢ for P25aDC. These fragments were ... 5¢-CACCATCACGGAGCATCCCCTGAG- 3¢,5¢-TCGCATCACCATCACCATCACGGAGCA-3¢ and 5¢-CACCCATGGGATCGCATCACCAT-3¢, and reverse primer 5¢-CACGGATCCCTACGTCACCCCTGA-3¢. After digestion with NcoI and BamHI...

Ngày tải lên: 14/02/2014, 21:20

... associated with loss -of- function or gain -of- function variants of MLL1 are prime exam- ples of the importance of maintaining the enzymatic activity of MLL1 under tight control. Identifying the protein ... dimethyl form of histone H3 [35], suggesting that the product specificity of the MLL1 core complex is that of a dimethyltransferase. Con- trary to expectations, kinetic exper...

Ngày tải lên: 16/02/2014, 14:20

... to aggre- gation during refolding, it can be used as a model with which to study chaperone activity in folding. Analysis of the nonassisted refolding of rhodanese showed the expected aggregation ... any time point. Table 1. Analysis of the aggregation rate during rhodanese refold- ing at pH 7.2. The rate of aggregation relative to the negative con- trol in the absence of bacitracin...

Ngày tải lên: 16/02/2014, 14:20

... Cu(II)- induced aggregation of Ab is toxic. The neurotoxicity of Cu(II)-induced Ab aggregation is related to the genera- tion of ROS through the redox cycling of Cu. In this regard, GIF has been ... localized Neuron GIF GIF Low GIF Reactive astrocyte Astrocyte ROS GIF GIF ROS Axon injury ABC Fig. 1. Diagrammatic model describing the possible physiological roles of GIF at differ- ent...

Ngày tải lên: 16/02/2014, 15:20

... crystal form of the complex with chagasin (PDB code: 3CBJ); and 22.5 A ˚ in the tetragonal crystal form of the complex with chagasin (PDB code: 3CBK) (Figs 3 and 4). The molecular weight of the ... can adopt a variety of con- formations, whereas the rest of the structure of cathep- sin B appears to be rigid. A comparison of the interaction constants of the binding of chagasi...

Ngày tải lên: 18/02/2014, 04:20

... cellulosome), anion-exchange chro- matography gave a single peak followed by a long tail. This suggests a random assembly of enzymes on the scaffoldin, leading to a large number of enzyme combi- nations. In ... be gathered in a large operon called the cip-cel cluster, beginning with the gene encoding the scaffolding pro- tein named cellulosome-integrating protein C (CipC), followed by gen...

Ngày tải lên: 18/02/2014, 08:20

... and regulatory structures of the systems. However, depending on the degree of biological knowledge and the amount of quantitative data, the models developed so far differ in their degree of granularity, ... approaches Andreas Kremling, Sophia Kremling and Katja Bettenbrock Systems Biology Group, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany Res...

Ngày tải lên: 18/02/2014, 13:20