báo cáo khoa học: "Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay" docx

... 1 of 15 (page number not for citation purposes) Journal of Nanobiotechnology Open Access Research Capillary electrophoresis for the characterization of quantum dots after non -selective or selective ... groups for conjugation) were principal contributors for the QD-biomolecule elec- trophoretic behavior. Various methods for bioconjuga- tion (selective and...

Ngày tải lên: 11/08/2014, 00:22

... important for the optimal activity of BsArb43B. Furthermore, to determine whether the role of the calcium is structural, thermal shift assays were per- formed to determine the T m of the protein in the ... absence of the calcium ion, using EDTA and EGTA as chelators, as described above. The sam- ples were incubated with both chelators for 24 h prior to the ther...

Ngày tải lên: 15/03/2014, 23:20

... for all the associated information. VISULEX offers two levels of detail. At the first level, the information is presented by using only the comments associated with the codes found. At the ... hindered by two factors : the dispersal of infor- mation and the obscurity of the coding. In ARIANE-78, the lexical data base may reside on much more 50 files, for...

Ngày tải lên: 17/03/2014, 19:21

... orientation of the side chain of Glu221. The second conformation of the side chain of Glu221, which also adopts two conformations but not correlated with the anomeric form of the bound mannose is shown ... dissolving it into the buffer used for the final dialysis of the protein. The errors on the sugar concentrations should be smaller than 2% for the oli...

Ngày tải lên: 23/03/2014, 11:20

... j chains and each HuVkFOR with a mixture of HuJkBACK for k chains. A GeneAmp PCR thermocycler (Perkin-Elmer 9600, Norwalk, CT, USA) was used for PCR. The condi- tions for the amplifications were: ... determine the corresponding germ lines. For clone 3F, VH3-VK3 were the closest families for V H and V L domains, respectively. In the case of C1, VH3- Vk1 were the fami...

Ngày tải lên: 23/03/2014, 13:20

... trate the use of the formalism in the areas of machine translation and reduction of FSs to canonical form. We conclude with an over- view of continuing strands of research. 2. Rule Format ... analysis of one FS with the synthesis of another; they may be thought of as a specialized variety of pattern- matching rule. They are local in nature, and permit th...

Ngày tải lên: 01/04/2014, 00:20

... from the egg white and homogenized after mixing with an equal volume of PBS. The homogenized egg yolk was then mixed with two volumes of chloroform and incubated for 2 hrs at room temperature. After ... 37℃ for 1 hr. Egg yolk antibodies were incubated with appropriate antigens at 37℃ for 1 hr after diluting with PBS at the appropriate concentration. After was...

Ngày tải lên: 07/08/2014, 15:20

... were similar for clone 431 (62.8%) or even higher for clone HV (28.4%) than those reported for microshoots originating from the base of the same trees (51.4 and 19.4% for clones 431 ... 1991). Therefore, the degree of reinvigoration depends on both the treatment being used and the characteristic being evaluated, sup- porting the qua...

Ngày tải lên: 08/08/2014, 18:21

... on the other hand, is homozygous for the « high » allele (A l ). All of the progeny from the standard cross have inherited the Al allele from the father and are, therefore, ... 13, which is homozygous for the A2 allele. With the exception of strains 10 and 5, the lines segregate into clusters according to their genetic makeup....

Ngày tải lên: 09/08/2014, 22:22

... being offered more hope for the disease-free survival time, there is still a substantial room for improving treatment. Tumor trans- formation into a more aggressive phenotype and develop- ment of ... been shown to interact with the hydrophobic cleft of MDM2, thus mimicking the binding of the helical portion of p53. However, one of the enanti- omers of this racemic...

Ngày tải lên: 10/08/2014, 22:20