... (SCN) method to examine gene expression of individual living cells using atomic force microscopy (AFM)without killing the cells. Results: In order to evaluate the SCN method, we compared the SCN method ... method with in situ hybridization (ISH). First, we examined spatial β-actin mRNA expression in single living cells with the SCN method, and then the same cells were subjected to ISH for β-actin mRNA. ... higher;quantity of β-actin mRNA detected by the SCN method increased more.Conclusion: In this study, we compare the SCN method with the ISH. We examined β-actinmRNA expression in single cells using both methods....