báo cáo khoa học: " Truncated forms of viral VP2 proteins fused to EGFP assemble into fluorescent parvovirus-like particles" doc

... left). MW EGFP -VP2- 14 EGFP -VP2- 23 EGFP -VP2- 40 VP2- 14 VP2- 23 VP2- 40 EGFP -VP2 VP2 A 85 46 118 B MW EGFP -VP2- 14 EGFP -VP2- 23 EGFP -VP2- 40 EGFP -VP2 anti-GFPanti -VP2 MW VP2- 14 VP2- 23 VP2- 40 VP2 C 66.2 45 D MW EGFP -VP2- 23 EGFP -VP2- 14 EGFP -VP2 EGFP -VP2- 40 anti-GFP 116 66.2 anti -VP2 85 46 118 Journal ... EGFP -VP2, EGFP -VP2- 14, EG...

Ngày tải lên: 11/08/2014, 00:22

... binding to CD4 and co-receptors and the fusion of the viral membrane with the plasma membrane of the cell. During passage through the cytosol, the viral RNA genome is reverse transcribed into DNA ... fusion of the viral envelope to the plasma membrane (B) lead to the retrograde transport along microtubules using the cytoplasmic the dynein motor. Viral capsid proteins m...

Ngày tải lên: 14/03/2014, 22:20

... Differential expression of the C(s) and Calpha1 isoforms of the cat- alytic subunit of cyclic 3’,5’-adenosine monophosphate- dependent protein kinase testicular cells. Biol Reprod 65, 151–164. 5 Orstavik S, Reinton ... ends due to alternative splicing of exon 1 and differential splicing of exons a, b and c. Interestingly, the N-terminus of Ca1 and Cb1 are more homologous to ea...

Ngày tải lên: 30/03/2014, 04:20

... inserted into the same restriction site of pTTQ18 [35]. To construct pNASC carrying lolC under P BAD ,a 1.2 kbp EcoRI–PstI fragment of pKM101 [10] was cloned into the same site of pMAN885EH [36]. To ... then cloned into the same site of pMAN885EH. To construct pNASE carrying lolE under P BAD ,a 1.3 kbp EcoRI–PstI fragment of pKM301 [10] was cloned into the same site o...

Ngày tải lên: 19/02/2014, 00:20

... assign, especially if one of the interacting partners (receptor N-domain) is not isotopi- cally labeled. Entropy of binding Discussion of entropic factors in terms of structure is less straightforward. ... ionization enthalpy of the buffer, and n is the net number of protons transferred during binding. To investigate whether there is a net proton transfer on IL-8 binding to th...

Ngày tải lên: 19/02/2014, 05:20

... adjusted to 90 lL with nuclease-free water. Reactions were started by pipetting 10 lL of the diluted enzyme solution into eight wells with a multichannel pipette to a total reaction volume of 100 ... and cell wall of Gram-negative bacteria do not restrict passage of ions, and the peri- plasmic proteins are, like the extracellular proteins, constantly exposed to the salinit...

Ngày tải lên: 19/02/2014, 05:20

... patient homozygous for a null mutation in the AUH gene may be due to the action of another mitochondrial hydratase, e.g. crotonase. This will need to be taken into consideration when the assay is used in a diag- nostic ... pre- pared by incubation of 3-methylcrotonyl-CoA with 3-methylcrotonyl-CoA-carboxylase in the presence of NaH 14 CO 3 . In this work, K m values for the hydrati...

Ngày tải lên: 19/02/2014, 07:20

... dichroism (CD) to investigate thermal unfolding of recombinant fibroblast isoforms of a-tropomyosin (Tm) in comparison with that of smooth muscle Tm. These two nonmuscle Tm isoforms 5a and 5b ... according to isoform type. In mam- malian cells, alternative splicing produces a variety of muscle and nonmuscle isoforms from four differ- ent genes [1]. Muscle cells express two major is...

Ngày tải lên: 19/02/2014, 07:20

... identification of the t ranscription cofactor vestigial- like p rotein 2 (UniProt AC: Q8BGW8; UniProt ID: VGL2_MOUSE). This cofactor of the t ranscription en- hancer factor TEF-1 appears to be a new ... VIIID1 2 to calsequestrin, mAb X IIC4 to sarcalu- menin, mAb IIH11 to the fast SERCA1 isoform of the sarcoplasmic reticulum Ca 2+ ATPase, mAb IIID5 to the a 1 -subunit of the...

Ngày tải lên: 19/02/2014, 16:20

... subsequent alkylation of mtDNA. The uptake of mitoDC-81 into cells driven by theplasmamembranepotential(Dw p ) followed by the further accu- mulation of mitoDC-81 into mitochondria driven by the mitoch- ondrial ... response of the electrode to cation concentra- Fig. 2. Uptake of mitoDC-81 by energized mitochondria. An ion- selective electrode was used to measure the uptake...

Ngày tải lên: 20/02/2014, 11:20