báo cáo khoa học: " Inorganic phosphate nanorods are a novel fluorescent label in cell biology" pdf

... Biology, Mayo Clinic Cancer Center, Mayo Clinic, Rochester, Minnesota, USA Email: Chitta Ranjan Patra - patra.chittaranjan@mayo.edu; Resham Bhattacharya - bhattacharya.resham@mayo.edu; Sujata Patra ... label in cell biology Chitta Ranjan Patra, Resham Bhattacharya, Sujata Patra, Sujit Basu, Priyabrata Mukherjee and Debabrata Mukhopadhyay* Address: Department of Biochemistry and Molec...

Ngày tải lên: 11/08/2014, 00:22

... of Pi and AC proteins on F-actin are also indicated by increased dissociation of Pi from actin filaments in the presence of Acanthamoeba actophorin and inhibited binding of this AC protein at high, ... of actin binding pro- teins and small molecules. The large family of actin depolymerizing factor (ADF) ⁄ cofilin (AC) proteins [1] has a central role in regulating actin dynamics. These s...

Ngày tải lên: 19/02/2014, 07:20

... catalytic mechanism was proposed for aminopeptidases that involves an acidic residue acting as a general acid ⁄ gen- eral base and a di-nuclear metal center participating in binding the substrate and stabilizing ... they are found in bacteria, plants and mammalian tissues. Many aminopeptidases are metallo-enzymes, containing two catalytic transition metals (usually zinc) in thei...

Ngày tải lên: 19/02/2014, 02:20

... CYP4x1 RNA in brain and in aorta, and this was confirmed by analysis of the EST database; this showed significant numbers of CYP4x1 ESTs in brain and aorta. An unexpected finding is that there was a high ... (DAB) staining was granular and confined to the cytoplasm of the cells. Based on their size and shape the cells labelled in all brain regions appeared to be neurons. Blood vessels w...

Ngày tải lên: 19/02/2014, 07:20

... the benefit of answer keys. So we used the heuristics to automatically create training data for a classifier by labelling each relevant document in the training set as an event narrative or a fleeting reference ... document. We trained an SVM classifier with the same bag-of- words feature set, using all relevant documents in the training set as positive instances and all irrelevant docume...

Ngày tải lên: 20/02/2014, 04:20

... were extracted, and the target Z I3 1A gene was amplified by PCR with primers 5¢-AAATA TAAAACGCTAGCGTCGACATGGCGC-3¢ and 5¢-AGC GTAAAGGATGGGGAAAG-3¢. The final ratio of target cells was determined by ... we have established a powerful approach to screen weak and transient protein–protein interactions by incorporating a novel signal amplifica- tion circuit with intact Gc as an artificial sig...

Ngày tải lên: 05/03/2014, 23:20

... Thus the L -arginine transport through the membrane exerts a regulatory role in the pathway L -arginine/NO. In most mammalian cells arginine requirements are met primarily by uptake of extracellular arginine ... protein intolerance (LPI). Hum. Mol. Gen. 9, 431–438. 42. Kamada, Y., Nagaretani, H., Tamura, S., Ohama, T., Maruyama, T.,Hiraoka,H.,Yamashita,S.,Yamada ,A. ,Kiso,S.,Inui,Y.,...

Ngày tải lên: 08/03/2014, 02:20

... (Tc00.1047053509891.40),F,5¢-GCCG TCATGCAAAAATATCC-3¢;R,5¢-CCTTTTCAGCCAA AAAGCTG-3¢; putative glucose-regulated protein 78 (Tc00.1047053506585.40),F,5¢-TGGCGGTAAGAAGAA GCAGT-3¢;R,5¢-CCGAGGTCAAACACAAGGAT-3¢; putative (H+)-ATPase G subunit (Tc00.1047053510993. 10),F,5¢-ACAACGTGCAAAGGCTTCTT-3 ... degradation, degraded or subjected to a combination of these processes. In mammalian cells, mRNA...

Ngày tải lên: 15/03/2014, 23:20

... concentration was determined with a Bio-Rad pro- tein assay kit using BSA as a standard according to the manufacturer’s protocol. Any bacterial and protease con- tamination of the purified protein preparations ... and analysed by silver staining as described in Materi- als and methods. Marker proteins and their corresponding molecular masss are indicated in lane 1. Lane 2, material f...

Ngày tải lên: 16/03/2014, 04:20

... of artemin indicated the lack of metal storage capacity. Artemin, apoferritin and ferritin inhibit citrate synthase denaturation Artemin, apoferritin and ferritin protected citrate syn- thase against ... River Canada (St Constant, Quebec, Canada) and cared for in accordance with guidelines in ‘Guide to the Care and Use of Experimental Animals’ available from the Canadian Council on Animal...

Ngày tải lên: 16/03/2014, 11:20