báo cáo khoa học: " Internalization of ferromagnetic nanowires by different living cells" pdf
... features of interest for each image. BioMed Central Page 1 of 11 (page number not for citation purposes) Journal of Nanobiotechnology Open Access Research Internalization of ferromagnetic nanowires by ... extent of cell alignment ver- sus the anisotropic orientation of the wires. Discussion The viability of the three types of cells is not significantly compromised by...
Ngày tải lên: 11/08/2014, 00:22
... cellular uptake by active internalization of extracellularly located cystatins has not been delineated. We demonstrated that cystatin C is internalized in five different cancer cell lines by using conceptually different ... conceptually different techniques such as CLSM, flow cytometry, western blotting, quantification of internalized inhibi- tor by immunological methods and me...
Ngày tải lên: 16/03/2014, 06:20
... part of an event separate from that of CRFR1 internalization. Agonist-mediated internalization of CRFR1 was dem- onstrated by visualizing CRFR1–EGFP conjugated recep- tor and by quantification of ... messenger product of either or both of these pathways could be invo lved in agonist-stimulated internalization of this receptor. The effect of PKA and PKC was investigate...
Ngày tải lên: 30/03/2014, 15:20
Tài liệu Báo cáo khoa học: Plasticity of laccase generated by homeologous recombination in yeast docx
... obtained by in vitro sequence permutation experi- ments and swapping of cupredoxin domains (D1, D2, D3). Such experiments are in progress. One of the beneficial effects of production of the present ... parame- ters for all the enzymes are of the same order of mag- nitude, the ratio of the total volumetric activities reflects a decrease in production by the hybrids of approxi...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx
... consisting of 0.63 g of MnCl 2 Æ4H 2 O, 60 mL of water, 60 mL of methanol and 4 mL of concen- trated sulfuric acid, briefly dried and heated at 100 °C for 20 min. SE were then quantified by densitometric ... min at 30 °C and terminated by the addition of 300 lL of chloroform ⁄ methanol (2 : 1, v ⁄ v). Lipids were extracted twice for 10 min with shaking using 300 lL of chloro...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: "Evaluation of Machine Translations by Reading Comprehension Tests and Subjective Judgments" doc
... appear likely to be of some use. Summary and Conclusions Evaluation of the quality of machine translations by means of a test of reading comprehension and by judg- ments of sentence clarity, ... judgments of the clarity of meaning of indivi- dual sentences. The approach through testing of reading comprehension provides a direct test of at least one aspect of...
Ngày tải lên: 19/02/2014, 19:20
Tài liệu Báo cáo khoa học: "Kinds of Features for Chinese Opinionated Information Retrieval" pdf
... Zagibalov Department of Informatics University of Sussex United Kingdom T.Zagibalov@sussex.ac.uk Abstract This paper presents the results of experi- ments in which we tested different kinds of features ... Selection Problem One of the central problems in Chinese NLP is what the basic unit 1 of processing should be. The problem is caused by a distinctive feature of the Chines...
Ngày tải lên: 20/02/2014, 12:20
Báo cáo khoa học: Stimulation of fibroblast proliferation by neokyotorphin requires Ca2+ influx and activation of PKA, CaMK II and MAPK/ERK pdf
... may be due to the pleiotropicity of the role of the sensitive forms of PKC in L929 cell proliferation [36]. In the presence of bisindolylmaleimide XI, the effect of neokyotorphin did not change. ... due to sustained acti- vation of MAPK ⁄ ERK [27,28]. The ability of neokyo- torphin to induce PC-12 cell differentiation remains Fig. 2. Proposed mechanism of action of neokyotor...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot
... Mops medium [5.5 gÆL )1 of Mops free acid, 5.1 gÆL )1 of Mops sodium salt, 0.5 g Æ L )1 of NaCl, 1 gÆL )1 of NH 4 Cl, 0.06 gÆ L )1 of Na 2 HPO 4 Æ2H 2 O, 0.05 gÆL )1 of KH 2 PO 4 , 2mm MgSO 4 , ... hypothesis that activa- tion of HbpR must proceed differently from that of XylR, a fusion protein between DA-HbpR and the A-domain of XylR was found to be incapable of acti- vat...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx
... the regulation of apop- tosis [21,22]. Thus, the possibility that the antiviral effect of spermine is mediated by induction of apop- tosis was analyzed. For this purpose, the effect of spermine ... in the absence and presence of spermine (Fig. 6, lanes 4 and 5). This shows that activation of caspases is an essen- tial component of the antiviral effect triggered by sper- mine...
Ngày tải lên: 07/03/2014, 21:20