... pUC19-VprYU-2 as a matrix by site-directed mutagenesis with specific primers: L23F-FACACTAGAG CTTTTTGAGGAGCTTAAG, L23F-R CTTAAGCTCCTCAAAAAGCTCTAGTGT, K27M-F CTTTTAGAGGAGCTTATGAGAGAAGCTGTTAG, ... envelopeis mediated by the interaction with the nucleoporin hCG1. JBiol Chem 2002, 277:45091-8.29. Nitahara-Kasahara Y, Kamata M, Yamamoto T, Zhang X, Miyamoto Y, Muneta K, Iijima S, Yoneda Y, Tsunetsugu-Yokota ... Terminator kit (Amer-sham) and a Genetic Analyzer (ABI3100 Applied Biosys-tems).Yeast two-hybrid assay The library of HIV-1 Vpr mutants fused to LexABD and the two-hybrid screening procedure of...