báo cáo khoa học: " Osteonecrosis of the jaw as a possible rare side effect of annual bisphosphonate administration for osteoporosis: A case report" ppsx
báo cáo khoa học: " Osteonecrosis of the jaw as a possible rare side effect of annual bisphosphonate administration for osteoporosis: A case report" ppsx
... 5:477http://www.jmedicalcasereports.com/content/5/1/477Page 3 of 4CAS E REP O R T Open Access Osteonecrosis of the jaw as a possible rare side effect of annual bisphosphonate administration for osteoporosis: A case ... side effects. To date, osteonecrosis of the jaw has notbeen linked to the annual administration of bisphosphonates. Case presentation: We report the case of a 65-year-old Caucasian woman suffering ... Association of Oral and Maxillofacial Surgeons: AmericanAssociation of Oral and Maxillofacial Surgeons position paper on bisphosphonate- related osteonecrosis of the jaws. J Oral Maxillofac Surg2007,...
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báo cáo khoa học: " Polymorphisms in the SULF1 gene are associated with early age of onset and survival of ovarian cancer" ppsx
... manufacturer’sinstructions. Each experiment was repeated three times.Statistical AnalysisStatistical analysis was performed using the Chi-squaretest or analysis of vari ance (ANOVA) analysis for cate-gorical variables ... by a Dual-Luciferase ReporterAssay System (Promega), and the relative luciferaseactivity was calculated as the ratio of firefly to renillaluciferase activity, according to the manufacturer’sinstructions. ... progression, accordingly. Disease recurrencewas defined as the r eappearance of any lesion that hadpreviously disappeared or the appearance of a newlesion that was histopathologically confirmed by a biopsy....
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Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf
... because of the absence of the catalyticsubunit ISP (Table 1). Figure 3A shows that a band of approximately 500 kDa was also found in this mutantstrain when the mitochondrial membranes were ana-lyzed ... were of analytical grade.Yeast strains and growth media The genotypes and sources of the S. cerevisiae strains aredescribed in Table 2. The ISP deletion strain wasprepared in accordance with the ... dimerization of the bc1complex atthis stage (i.e. just after the addition of ISP and Qcr10p).On the other hand, in the transition from the 500 kDaband to the 670 kDa band, a structural rearrangementof...
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Tài liệu Báo cáo khoa học: Acetylcholinesterase from the invertebrate Ciona intestinalis is capable of assembling into asymmetric forms when co-expressed with vertebrate collagenic tail peptide doc
... 2157–2167.8 Satou Y, Yamada L, Mochizuki Y, Takatori N,Kawashima T, Sasaki A, Hamaguchi M, Awazu S,Yagi K, Sasakura Y et al. (2002) A cDNA resourcefrom the basal chordate Ciona intestinalis. Genesis ... sequence data are also available on the DOE JGI Database. The amino acid sequence for the protein has also been deposited in the Esther database as cioin-acche1 (http://bioweb.ensam.inra.fr/ESTHER/general?what=index) ... the appropriate interactions between the WAT domain of the catalytic subunit and the PRAD domain of ColQwere present to assemble the catalytic tetramers of the asymmetric forms. The A 12form...
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... that the calcium-binding event of lower affinity is attributable to the catalytic site of PLDa2, as found for PLDb from A. thaliana [39]. The decrease in PLD activity at concentrationsabove the ... determinedat about 10-fold lower protein concentration than the measurements of inactivation, show the sametrends with respect to the effects of NaCl, CaCl2andEDTA as the inactivation constants. As ... m)1Æcm)1[40]. Other PLDa2 concentrations weremeasured by the BCA assay (Pierce, Rockford, IL, USA)with BSA as standard.SDS ⁄ PAGESDS ⁄ PAGE was performed according to the method of Laemmli [41]....
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... 1162–1168.13 Nakayama M, Takahashi K, Kitamuro T, YasumotoK, Katayose D, Shirato K, Fujii-Kuriyama Y & Shiba-hara S (2000) Repression of heme oxygenase-1 byhypoxia in vascular endothelial cells. ... Kolpakov FA et al. (1998) Databases ontranscriptional regulation: TRANSFAC, TRRD andCOMPEL. Nucleic Acids Res 26, 362–367.47 Takahashi S, Takahashi Y, Ito K, Nagano T,Shibahara S & Miura T ... HepG2 and HeLa cellswere cultured for 48 h under normoxia or hypoxia, and then harves-ted. The microsome fraction was prepared and used for the assay of HO activity. The data are means ± SEM of...
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Tài liệu Báo cáo khoa học: Diversity and junction residues as hotspots of binding energy in an antibody neutralizing the dengue virus doc
... sulfate, 5 mm imidazole, and their periplasmicfluid was prepared as above. The preparation of MalE-E3-H6, partially purified byaffinity chromatography on amylose resin (see above), and the periplasmic ... surface wasregenerated by injecting 5 lLof50mm HCl. The kineticdata were analysed with the biaevaluation 3.0 software(Biacore). The active concentration of each Fab4E11-H6preparation was determined ... MalE-E3-H6 wasimmobilized in the wells of a microtiter plate and the boundFab4E11-H6 was revealed with a goat antibody, directed againstmouse Fab and conjugated with alkaline phosphatase. The...
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Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... introduction of the beta branched side- chain where the unbranched Met side- chain is ordinarily packed. The physical basis for the reduced stability of the T45Smutant may have a similar explanation. Although ... spectra evidenced a great loss of secondary structure for M88V and T45S inurea. In the absence of urea, the signal was smaller for the M88V mutant than for the other mutants and WT bacterio-phage, ... on the samples was ana-lyzed at room temperature. (A) The effect was measured by the tryp-tophan fluorescence emission of the spectral center of mass. The samples were excited at 280 nm and the...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... CTGACCTtcTGGCATAACACCGGtGAAATCAgeIW32F CCTGGTtcCATAACACCGGtGAAATCAgeIW240F GGTGCTgAGCTCAAGTGTGACTTtcGTCTACSacIW402F CCGGTGGTcGAcTTTGGTTtcACGCCCSalIE273Q ACGTACTGCTgTCCGGAAAGtACtCCATGGCCGCScaID353N ... ACGTACTGCTgTCCGGAAAGtACtCCATGGCCGCScaID353N TCCAATCCGTtAGTCTGgCCaTAGAACGCGCMscIE356Q GGAGAATGGTgCCAGGGTACATTTcCAATCCGTCAKpnID372N AATACATCTTaAGGCCGTCGTtGTCGGTGTGG A IID373N AATACATCTTaAGGCCGTtGCGTCGGTG A IIÓ FEBS ... catalyst of pK a 5.9,and that the adjacent Glu180 is negatively charged, raising the p K a of the general acid catalyst [23]. I t is likely t hatcatalytic residues of IPU adopt a similar catalytic...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... between Asn149 and the a- carboxylate group of arginine were also operative for arginase II, both the lack of arginase activity as well as the resistance of the Asn149Asp mutant toinhibition by l-arginine, ... fully active state. In contrast, the arginaseactivity of the Asn149Asp variant was practicallyundetectable, both before and after the incubation with the manganese ions.Fully active His120Asn and ... considered for arginase and agmatinase[20]. Such substrate discrimination is particularlyimportant for mammalian arginase II and agmatinase, as both enzymes are mitochondrial and functionallydifferent...
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