báo cáo khoa học: "Molecular prognostic markers for adult acute myeloid leukemia with normal cytogenetics" ppsx

... favorable for CROR 20.8BioMed CentralPage 1 of 10(page number not for citation purposes)Journal of Hematology & OncologyOpen AccessReviewMolecular prognostic markers for adult acute myeloid ... Bloomfield CD: Independent confirmationof a prognostic gene-expression signature in adult acute myeloid leukemia with a normal karyotype: a Cancer and Leukemia Group B study. Blood 2006, 108:1677-1683.61. ... Kreitmeier S, Tobis K,Döhner H, Döhner K: Prognostic significance of activatingFLT3 mutations in younger adults (16 to 60 years) with acute myeloid leukemia and normal cytogenetics: a study of theAML...

Báo cáo khoa học: "Molecular cloning of the cDNA of canine homeodomaininteracting protein kinase 2" ppsx

... and 96% identity with those of human and mouse, respectively. Thededuced amino acid sequence of canine HIPK2 has alsoall domains’ sites compared with human and mouseHIPK2. Therefore, these structural ... and p53. In dogs, the gene therapy with p53in cancer patients is in experimental stage.The study about the nucleotide sequence of canine HIPK2was performed for the development of cancer therapybecause ... was disrupted in 1.5 ml tube with 350 µl of lysis buffer (Macherey-Nagel, Germany) andwas ground with automatic homogenizer. Total RNA wasisolated from spinal tissue with RNA extraction kit(Macherey-Nagel,...

báo cáo khoa học: "Ring chromosome 18 abnormality in acute myelogenous leukemia: the clinical dilemma" ppt

... and prognosis in adult acute myeloid leukemia. Hematology Am Soc Hematol Educ Program 2006, 169-77.3. Dohner H, et al: Diagnosis and management of acute myeloid leukemia in adults: recommendations ... in New Zealandpatients with acute myeloid leukemia. Cancer Genet Cytogenet 1983,8(1):51-66.14. Mrozek K, et al: Spectral karyotyping in patients with acute myeloid leukemia and a complex karyotype ... cytogenetic abnormalities are predictive ofinduction success, cumulative incidence of relapse, and overall survivalin adult patients with de novo acute myeloid leukemia: results fromCancer and Leukemia...

báo cáo khoa học: " Supernumerary, ectopic tooth in the maxillary antrum presenting with recurrent haemoptysis" ppsx

... presented with haemoptysis.Case presentation: A 45-year-old male presented with a 2-month history of episodic haemoptysis. A pedunculatedgrowth from the inferior nasal turbinate was seen with fibre-optic ... com-monly seen within the maxillary sinus [3].We hereby describe a case of a 45-year-old man with a supernumerary, ectopic molar tooth in the left maxil-lary antrum who initially presented with haemoptysis.To ... bone. (Figure 1).Based on these findings, the patient was scheduled for the endoscopic removal of a potential foreign bodyalong with debridement of the left maxillary sinus. Lim-ited pre-operative...

Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulﬁde isomerases as molecular chaperones for seed storage proteins doc

... pretreated with dithiothrei-tol (C) or tunicamycin (D) and labeled with Pro-mixL-[35S] in vitrolabeling mix for 1 h. After labeling, microsomes were isolated andtreated with (+) or without ... GmPDIL-2 with each speciﬁc antibody.Microsomes were isolated from cotyledons (150 mg beans) andtreated with (+) or without ()) DSP. Proteins were extracted andimmunoprecipitated with anti-GmPDIL-1 ... associated with GmPDIL-1 and GmPDIL-2 was difﬁcult, as only afew proglycinin molecules may associate transiently with PDI proteins in the ER. Then, similar experi-ments were performed with dithiothreitol-treated...

Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identiﬁes a calcium sensitive region required for basal activity doc

... [4] and bycomparison with the corresponding mouse [26] and human[27] genes. The sequence on either side of each exon–intronjunction (Table 1) is conform to the GT/AG rule for splicedonor and ... analysis using EcoRI restriction diges-tion is consistent with the presence of a single copy gene for xMGP (Fig. 3). However, Southern analysis with BamHI(Fig. 3 A) shows additional fragments that ... othose obtained with the positive control (Fig. 5 a nd data notshown). Cotransfection experiments us ing the xMGPpromoter constructs in combination with expression plas-mids for mammalian nuclear...

Báo cáo khoa học: Molecular basis of the unusual catalytic preference for GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase doc

... Kmvalues for ATP were in agree-ment with the results obtained for ADP in the forwardreaction. It is worth noting that although changes incatalytic efﬁciency were not signiﬁcant for GTP in ... afﬁnities for otherpurine nucleotides such as GTP or ITP to thoseobserved with ADP ⁄ ATP; however, the phosphoryla-tion transfer rates displayed with GTP or ITP are onaverage 50% lower than with ... catalysis with ADP ⁄ ATP as substrates; the cause of this behav-ior was the higher Kmvalues for the adenine nucleo-tides (at least 10-fold) compared with the Kmvaluesexhibited with GDP...

Báo cáo khoa học: Molecular basis for speciﬁcities of reactivating factors for adenosylcobalamin-dependent diol and glycerol dehydratases pptx

... determines the speciﬁcity for a dehydratasealso became clear in this study.Why does DDR cross-form a complex with glyceroldehydratase, and why does GDR not cross-form acomplex with diol dehydratase? ... subunits were per-formed with the EBI SSM [56] under the pairwise three-dimensional alignment mode. Buried molecular surfaceareas were calculated with the program CNS [57], for whichthe probe ... structure,were 120 500 m)1Æcm)1 for diol dehydratase [35], 112 100m)1Æcm)1 for glycerol dehydratase [49], 58 140 m)1Æcm)1 for DDR [35], and 86 500 m)1Æcm)1 for GDR [37], respectively.Assays...

Báo cáo khoa học: Molecular dynamics of the DNA-binding domain of the papillomavirus E2 transcriptional regulator uncover differential properties for DNA target accommodation pdf

... EM1 with position restraints 10 000 5000 EM2 with position restraints 20 000 50012.5 MD1 with position restraints 25 000 of 0.5 fs 5000 EM3 with position restraints 15 000 2525.0 MD2 with ... second generation forceﬁeld for the simulations of proteins, nucleic acids andorganic molecules. J Am Chem Soc 117, 5179–5197.33 Ponder JW & Case DA (2003) Force ﬁelds for proteinsimulations. ... system for the investigation of such issues, because there aremany viral strains that have coevolved with their ver-tebrate hosts for over a 100 million years, providing adatabase for the...

Báo cáo khoa học: Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation potx

... undergo normal e mbryo-genesis, but develop microvesicular hepatic steatosis within4 d ays of b irth, dying by the end of two weeks with fattyliver [25]. Conditional gene knockout may therefore ... These kineticparameters were also in agreement with previously reportedvalues for other forms of AK [8].Most tissues express more of one AK isoformthan the otherQuantitative immunoblot analysis ... (Novagen). Primers: 5¢-CGTGGGGTGCATATGGCAGCTGCG for the 5 ¢ end of mAK-L;5¢-GTAGGTGCACATATGACGTCCACC for t he 5¢ endof mAK-S; 5¢-ATATAGGATCCTCAGTGGAAGTCTGG for the 3¢ end of both clones. Consensus...

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