... antioxidant levels, in response t o theinhibition of GA expression, are presented in Table 1. Theinhibition of GA expression decreases the level of GSH,resulting in a 40% re duction of the GSH/GSSG ... growth of EATC and 0.28AS-2 cells during treatment with MTX and H2O2was investigated by using the MTT assay. First, we analysedthe growth curve of EATC and 0 .28AS-2 cells at baseline, and ... simil ar intracellularglutamate concentrations, 0 .28AS-2 cells have significantlyhigher glutamine levels, reflecting the strong inhibition of GA expression (Table 2). The diminished mitochondrialglutamine...