... Horse-radish peroxidase-conjugated antibody against caspase-3(#610325) was from BD Pharmigen, and antibody againstb-actin (A5 441) was from Sigma.Statistical analysisAll data are expressed as the ... for these differences, we examined the main steps involved, following the uptake of satu-rated and unsaturated FFAs into the cells. After theirinternalization, FFAs are converted to fatty acyl-CoA, a ... synthesis in the endoplasmic retic-ulum, apparently because of the formation of a pool of oversaturated inter-mediates, represents the key initiating event in the mechanism of saturatedfatty...