báo cáo khoa học: "Improved retroviral suicide gene transfer in colon cancer cell lines after cell synchronization with methotrexate" docx

... target cells before retroviral gene transfer. Methods Cell culture We used two colon cancer cell lines: the human HT29 and the murine DHDK12 pro-b (Pr. Martin, Dijon; France) cell lines. Cell lines ... the transfer efficiency of a retroviral- mediated gene encoding herpes simplex virus thymidine kinase (HSV-tk) in two colon cancer cell lines, DHDK12 and HT2...

Ngày tải lên: 10/08/2014, 10:21

... of HEK293 cells. This finding suggests the absence or delay of promoter silencing, as some previous data have shown that silencing occurs within the first 6 h or during the first week after gene transfer ... promoter silencing does not happen in particular cases, including our study, remains unknown. However, in vitro [60] and in vivo [61] studies have shown that AAV vectors induce a...

Ngày tải lên: 29/03/2014, 21:20

... exam- ined in studies evaluating the role of vitamin D on MS [9]. Several such studies that have investigated the role of VDR gene polymorphisms in various ethnic groups have produced conflicting ... associations of the VDR gene poly- morphisms, such as a significant interaction between winter sun exposure during childhood [13], or interaction between dietary intake of vitamin D and V...

Ngày tải lên: 11/08/2014, 07:21

... Principal Component Analysis (PCA) of GS genes and genes involved in lignin biosynthesis and C1 metabolism. The expression profiles of GS genes were examined in silico during wood formation in ... regions containing intermediate and mature leaves (A2 and A3) and in R2. Interestingly, maximum levels of PtGS1.1 expression were observed in the leaf lamina (L2, L3) with decreased abun...

Ngày tải lên: 11/08/2014, 11:21

... and G a15 . Strains were grown in minimal media with 2% glucose (uninduced) or sub- sequently in minimal media with 2% galactose (induced) at 30 or 15 °C. Differential bioluminescence for each ... water solution for 1 h, washed with water then with 0.1 m HCl (10 min) and again with water. Free aldehydic sites were quenched by incubation with 2% (w ⁄ v) glycine in NaCl ⁄ P i...

Ngày tải lên: 07/03/2014, 16:20

... ozaki@bio.sci.osaka-u.ac.jp Abbreviations: CRALBP, cellular retinal-binding protein; CRBP, cellular retinol-binding protein; IRBP, interphotoreceptor retinoid-binding protein; RBP, retinol-binding protein. Note: The nucleotide ... soluble in water, hydrophilic retinoid-binding proteins are therefore required for stabilizing retinoids in the watery plasma as well as in the cytoplasm, and...

Ngày tải lên: 17/03/2014, 03:20

... Na + concentration followed sodium pump inhibition by ouabain with a subsequent gradual increase or oscillations in intracellular Ca 2+ con- centration. Whether this increase in intracellular Ca 2+ concentration ... in [Na + ] i resulting in reduced Na + /Ca 2+ - exchange locally. Cellular compartments with larger chan- ges in intracellular Na + and Ca 2+ may thus be the result...

Ngày tải lên: 23/03/2014, 17:21

... (tyro- sine phosphorylation) b-catenin, a fact that precludes its binding to a-catenin and E-cadherin, thus pre- venting cell adhesion and facilitating spreading. Phosphorylation of b-catenin targets ... regulation of gene expression, proteolytic pathways, protein metabolism, maintainers of cytoske- leton organization, cell cell signalling, regulation of cell cell communication and re...

Ngày tải lên: 30/03/2014, 15:20

... table were less affected by the winter decrease in rainfall. The main driving force in gallery forest ecosys- tems is the water flow, since it results in changes in soil properties, and allows ... resource inventories. In Proceedings International Symposium on Assessment and Monitoring of Forests in tropical Dry Regions with Special Reference to Gallery Forests, Imaña-Encinas J....

Ngày tải lên: 08/08/2014, 14:21

... The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. ... lesion in each specimen. On the other hand, staining with antibodies against p53 revealed that recurrent cancer cells had higher expression of mutant p53 than primary cancer cells di...

Ngày tải lên: 09/08/2014, 03:23