báo cáo khoa học: " Effects of RNA interference-mediated gene silencing of JMJD2A on human breast cancer cell line MDA-MB-231 in vitro" pptx

... 30:90 http://www.jeccr.com/content/30/1/90 Page 8 of 9 RESEARC H Open Access Effects of RNA interference-mediated gene silencing of JMJD2A on human breast cancer cell line MDA-MB-231 in vitro Bei-Xu Li, Ming-Chang ... demethylation of H3K9me3. The aim of this study was to investigate depressive effects on JMJD2A by transfection with JMJD2A- sepcifi...

Ngày tải lên: 10/08/2014, 10:21

... pathway. Gene disruption of CYM1 thereby resulted in an increase in t he amount of secr eted proCCK. Interestingly, gene disruption of CYM1 also resulted in increased secretion of other hormones ... deubiquit- inating enzyme Ubp16, a metalloprotease belonging to the large f amily of deubiquitinating enzymes, mainly consisting of cysteine proteases [43]. Another class of...

Ngày tải lên: 07/03/2014, 16:20

... as: Zhang et al.: Effects of metastasis-associated in colon cancer 1 inhibition by small hairpin RNA on ovarian carcinoma OVCAR-3 cells. Journal of Experimental & Clinical Cancer Research 2011 30:83. Submit ... expression of MACC1 in OVCAR-3 cells. We also successfully obtained O VCAR-3 cell line with the best inhibitory effects of MACC1 expression for further...

Ngày tải lên: 10/08/2014, 10:21

... Solid line: the case of kinase sequestration, dotted line: no kinase sequestration. Grey lines indicate the stimu- lus (i.e. kinase concentration), scaled by 0.1 in (A). Effects of sequestration ... response of the active fraction to a change of the kinase concentration as a function of the concentra- tions of the two forms ([T] and [T*]) and the elastici- ties of the enzy...

Ngày tải lên: 19/02/2014, 07:20

... no protein binding detected by the DMS methylation assay in the MMTV promoter in the noninduced state. Addition of hormone resulted in a drastic reduction in DMS methylation (protection) over ... nucleosome repositioning during hormone induction [6] is not solely explained by GR binding to the GRE sequences within the nucleosome B region, but also depends on the subsequent histone...

Ngày tải lên: 19/02/2014, 12:20

... domains in the relevant variant of the hinged conventional hairpin ribozyme leading to enrichment of an extended inactive confor- mation [34,35]. Because of the single-stranded linker, joining ... Journal 272 (2005) 4464–4474 ª 2005 FEBS 4465 Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined...

Ngày tải lên: 20/02/2014, 01:20

... secretion [6]. Addi- tionally, arginine methylation regulates cytokine gene transcription in T helper (Th) cells through arginine methylation of the NFAT cofactor, NFAT interacting protein 45 ... activity and cellular protein arginine methylation, including methyl- ation of the guanine nucleotide exchange factor Vav1 [5]. Incubation with MDL 28,842 diminished methyla- tion of the gu...

Ngày tải lên: 06/03/2014, 11:20

... results for a common query. Indexing millions of search logs and listings can be done in minutes. Reusing text search engines allows a seamless integration between query parsing and search. We ... in a sin- gle utterance 1 . Finally, many queries often con- tain other constraints (assuming LocationTerm is a constraint) such as that deliver in restaurants that deliver or open 24 hours...

Ngày tải lên: 08/03/2014, 21:20

... was measured. Inhibition of the complex was evident in both cell lines, but predominantly in U87 MG cells, and was accompanied by accumulation of ubiquitin conjugates. Accumulation of p53 and Bax, ... The two cell lines are different with respect to a single mutation in the p53 gene; this characterizes the T98G line, whereas the U87 MG line maintains the wild-type form of...

Ngày tải lên: 15/03/2014, 11:20

... hydrogen bond donor, interacting with the carbonyl oxygen of the P4 residue on the inhibitor, or as a hydrogen bond acceptor for the P4 NH of the inhibitor. Although some of the inhibitors were only ... recircularization. The vials were sub- jected to 1 min of melting at 95 °C, 1 min of annealing at 73 °C and 15 min of extension at 72 °C in 25 cycles using a thermal cycler (Ge...

Ngày tải lên: 16/03/2014, 06:20