... this article as: Xu et al.: Small interference RNA targeting tissue
factor inhibits human lung adenocarcinoma growth in vitro and in vivo.
Journal of Experimental & Clinical Cancer Research 2011 ... RESEARC H Open Access
Small interference RNA targeting tissue factor
inhibits human lung adenocarcinoma growth
in vitro and in vivo...
... by
the joint action of machinery that includes clathrin
heavy and light chains, adaptor complexes (AP1 and
AP2), membrane-bending proteins such as epsin, amphi-
physin, syndapin and endophilin A, ... clathrin heavy chain and dynamin inhi-
bits uptake by 70% or more (Figure 2 and Additional
File 2). In addition, knockdown of several other endocy-
tic proteins includ ing epsin, synd...
... producing siRNA using the
polymerase III promoter offer more efficient siRNA delivery and are showing promise both in
vitro and in vivo [25]. In our research, adenovirus-mediated siRNA targeting ... mRNA and protein in CRC cells and normal human intestinal epithelial cell line. Then,
adenovirus-mediated RNA interference technique was employed to inhibit the express...
... was
observed during this kind of shift experiment in B. sub-
tilis AmtB-deficient and GlnK-deficient strains, despite
TnrA being soluble in these cells [12,15]. To gain dee-
per insights in the involvement ... collected, and after rigorous washing in nonionic
detergent-containing buffer and elution of antibody-
bound protein, the samples were separated by
SDS ⁄ PAGE and analyzed...
... lgÆmL
)1
,andtheblots
washed and incubated with diaminobenzidine and hydrogen
peroxide. A combination of the biotinylated lectins Sambu-
cus nigra agglutinin (SNA; 10 lgÆmL
)1
)andMaackia
amurensis agglutinin (MAA; ... differing values of K
m
and k
cat
.
Fig. 9. pH profile of human skin and lung tryptase in the presence and
absence of heparin. (j) skin tryptase, no heparin (h)...
... expressed in
human tissues and cell lines, and high endogenous
expression of JMJD2A mRNA was found in several cell
types, including human T-cell lymphotropic virus 1-
infected cell lines, the ... well and incubated in RPMI
1640 medium (GIBCO, Invitrogen, USA) conta ining
10% FBS (GIBCO, Invitrogen, USA). RPMI 1640 med-
ium containing 10% FBS was replaced by serum-free
Opti-MEM...
... investing time and effort in preparing and
analyzing the full-length protein can be extremely
rewarding, as shown by the information obtained with
the tumor suppressor protein BRCA2 and its interac-
tion ... structure facilitating protein bind-
ing (indirect) and specific amino acid–base contacts
(direct). A recent examination of proteinÆnucleic acid
structures has shown how minor gr...
... for silencing at telomeres, rDNA
and the silent mating type loci, and for maintaining
genome integrity [5,6]. In addition to silencing, Sir2
activity is linked to lifespan extension in yeast [7],
worms ... 26
was not as potent as splitomicin in inhibiting ySir2. In
addition to studies in yeast, sirtinol and splitomicin
have been used as general sirtuin inhibitors in mamma-
l...
... fluorescent and affinity labeling of RNA
using a small engineered ribozyme. Chembiochem
in press.
19 Feldstein A, Buzayan JM & Bruening G (1989) Two
sequences participating in the autolytic processing ... hairpin ribozyme by dissecting the two
domains at the hinge between helix 2 and helix 3 and
rejoining helix 4 to helix 1 via a linker of six unpaired
residues [27] (Fig. 1)....