báo cáo khoa học: " Small interference RNA targeting tissue factor inhibits human lung adenocarcinoma growth in vitro and in vivo" ppsx

... this article as: Xu et al.: Small interference RNA targeting tissue factor inhibits human lung adenocarcinoma growth in vitro and in vivo. Journal of Experimental & Clinical Cancer Research 2011 ... RESEARC H Open Access Small interference RNA targeting tissue factor inhibits human lung adenocarcinoma growth in vitro and in vivo...

Ngày tải lên: 10/08/2014, 10:21

... by the joint action of machinery that includes clathrin heavy and light chains, adaptor complexes (AP1 and AP2), membrane-bending proteins such as epsin, amphi- physin, syndapin and endophilin A, ... clathrin heavy chain and dynamin inhi- bits uptake by 70% or more (Figure 2 and Additional File 2). In addition, knockdown of several other endocy- tic proteins includ ing epsin, synd...

Ngày tải lên: 12/08/2014, 04:21

... producing siRNA using the polymerase III promoter offer more efficient siRNA delivery and are showing promise both in vitro and in vivo [25]. In our research, adenovirus-mediated siRNA targeting ... mRNA and protein in CRC cells and normal human intestinal epithelial cell line. Then, adenovirus-mediated RNA interference technique was employed to inhibit the express...

Ngày tải lên: 09/08/2014, 02:21

... was observed during this kind of shift experiment in B. sub- tilis AmtB-deficient and GlnK-deficient strains, despite TnrA being soluble in these cells [12,15]. To gain dee- per insights in the involvement ... collected, and after rigorous washing in nonionic detergent-containing buffer and elution of antibody- bound protein, the samples were separated by SDS ⁄ PAGE and analyzed...

Ngày tải lên: 06/03/2014, 00:21

... lgÆmL )1 ,andtheblots washed and incubated with diaminobenzidine and hydrogen peroxide. A combination of the biotinylated lectins Sambu- cus nigra agglutinin (SNA; 10 lgÆmL )1 )andMaackia amurensis agglutinin (MAA; ... differing values of K m and k cat . Fig. 9. pH profile of human skin and lung tryptase in the presence and absence of heparin. (j) skin tryptase, no heparin (h)...

Ngày tải lên: 31/03/2014, 07:20

Ngày tải lên: 11/08/2014, 00:23

... expressed in human tissues and cell lines, and high endogenous expression of JMJD2A mRNA was found in several cell types, including human T-cell lymphotropic virus 1- infected cell lines, the ... well and incubated in RPMI 1640 medium (GIBCO, Invitrogen, USA) conta ining 10% FBS (GIBCO, Invitrogen, USA). RPMI 1640 med- ium containing 10% FBS was replaced by serum-free Opti-MEM...

Ngày tải lên: 10/08/2014, 10:21

... investing time and effort in preparing and analyzing the full-length protein can be extremely rewarding, as shown by the information obtained with the tumor suppressor protein BRCA2 and its interac- tion ... structure facilitating protein bind- ing (indirect) and specific amino acid–base contacts (direct). A recent examination of proteinÆnucleic acid structures has shown how minor gr...

Ngày tải lên: 14/02/2014, 19:20

... for silencing at telomeres, rDNA and the silent mating type loci, and for maintaining genome integrity [5,6]. In addition to silencing, Sir2 activity is linked to lifespan extension in yeast [7], worms ... 26 was not as potent as splitomicin in inhibiting ySir2. In addition to studies in yeast, sirtinol and splitomicin have been used as general sirtuin inhibitors in mamma- l...

Ngày tải lên: 20/02/2014, 01:20

... fluorescent and affinity labeling of RNA using a small engineered ribozyme. Chembiochem in press. 19 Feldstein A, Buzayan JM & Bruening G (1989) Two sequences participating in the autolytic processing ... hairpin ribozyme by dissecting the two domains at the hinge between helix 2 and helix 3 and rejoining helix 4 to helix 1 via a linker of six unpaired residues [27] (Fig. 1)....

Ngày tải lên: 20/02/2014, 01:20