báo cáo khoa học: "ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF-7 in vitro" pptx

... AccessShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF-7 in vitroQingming Jiang†, ... levels of MTA1, protein expression of ER alpha, MMP-9, cyclinD1 and the changes of cancer cells invasiveness, proliferation, cells cycle were no statistical difference in ER- positive human breast cancer ... the protein expression of MMP-9 and cyclinD1 in ER- negativehuman breast cancer cell lines MDA-MB-231. Thesilence effect of MTA1 could efficiently inhibit the inva-sion and proliferation of MDA-MB-231...

báo cáo khoa học: " Development-dependent changes in the tight DNA-protein complexes of barley on chromosome and gene level" pot

... decrease of TBP binding along the whole gene, especially in the promoter region and intron II. Expression of the Bmy1 gene coupled to ripening was followed by release of the exon III and intron III ... alpha-globin gene domain [7]. Comparison of TBP distribution in the domain in erythroid cells differing in the degree of alpha-globin gene transcription revealed changes in distri-bution of TBPs, ... decrease in the number of genomicsites involved in interaction with TBPs. In the stage 10coleoptiles only one marker of 1H and two markers of 7Hwere found in the R2 fraction, some markers disappearedfrom...

báo cáo khoa học: " Using single cell cultivation system for on-chip monitoring of the interdivision timer in Chlamydomonas reinhardtii cell cycle" pps

... examined th e time course of changes in cell volumeduring the interdivision phase under different conditions of uninterrupted continuous light exposure. We foundthat the rate of increase in cell ... triggers a cell to enter thedivision phase at an interdivision time T =1.4/μ.Wecall this new interdivision control mechanism the ‘inter-division timer’.Regulation of the interdivision phase ... volume-based‘interdivision timer’We also investigated whether re-exposing cells to lightwould have an effect on the duration of the interdivisionphasebythe‘interdivision timer’. This was examinedusing...

Báo cáo khoa học: A (1ﬁ3)-b-D-glucan recognition protein from the sponge Suberites domuncula Mediated activation of ﬁbrinogen-like protein and epidermal growth factor gene expression pot

... the design of degenerate primers. Aligning different ﬁbrinogen-domain containing proteins, ﬁbrinogens, ﬁbroleukins/techylectins, angiopoietins, ﬁcolins and tenascins, thefollowing consensus ... present in the sponge protein. It is proposed that thissponge protein represents the ancestor of all ﬁbrinogendomain-containing proteins found in Metazoa. In conse-quence, the other vertebrate ... level of expression; onecodes for a ﬁbrinogen-like protein and the other for theepidermal growth factor precursor. Expression of the(1ﬁ3)-b-D-glucan-binding protein and the ﬁbrinogen-likeprotein...

Báo cáo khoa học: Differential gene expression proﬁles of red and green forms of Perilla frutescens leading to comprehensive identiﬁcation of anthocyanin biosynthetic genes doc

... GST-like gene involved in vacuoletransport of anthocyanin rescued the lesion of anthocyanin accumulation in tt19, indicating a function of PfGST1 in vacuole sequestration of antho-cyanin in perilla. ... deter-mined in vitro using naringenin chalcone as the sub-strate. As shown in Fig. 8, the recombinant PfCHI1 protein could stereospeciﬁcally convert naringeninchalcone to (2S)-naringenin, whereas ... the expression of maize Bz2 and petunia AN9 [20], indicating again the universalnecessity of GST-like proteins in anthocyanin accumu-lation and the interspecies functional compatibility of these...

Báo cáo khoa học: Myocyte enhancer factor 2 (MEF2) is a key modulator of the expression of the prothoracicotropic hormone gene in the silkworm, Bombyx mori ppt

... wandering is photoperiodically controlled in B. mori [3]. In addition, in larvae of Heliothis vires-cens, expression of the PTTH gene declines sharply atthe onset of larval wandering behavior and ... Effect of PTTH gene expression on the overexpression and silencing of the BmMEF2 gene. RT-PCR was performed on pupalbrain injected each of three recombinant AcNPVs (v[PT ⁄ EGFP],v[PT ⁄ MEFs] and ... eachhemisphere [14].Regulation of PTTH gene expression by controllingBmMEF2 expression To investigate whether the expression of BmMEF2affects PTTH gene expression, we constructed tworecombinant...

báo cáo khoa học: " The gene map of the rabbit α and β casein gene synteny" pps

... the translation products of the major mRNA species from rabbit lactating mammary gland and construction of bacterialrecombinants containing casein and a lactalbumin complementary ... Polymorphism of milk proteins : some biochemical and genetical aspects.Proceedings of the l6th International Conference on animal blood groups and biochemicalpolymorphisms. Leningrad ... markers, we find that a and f3 casein genes and the whey acidic protein gene are not syntenic with any of the 20 other chromosome markers already investigated in our laboratory.II....

báo cáo khoa học: " HC-Pro silencing suppressor significantly alters the gene expression profile in tobacco leaves and flowers" docx

... e.g. genes encod-ing proteases and protease inhibitors (cysteine proteinase,serine carboxypeptidase and trypsin inhibitor), stru cturalproteins (proline rich proteins), and other proteins acting on ... protein has domains of a cysteine endopeptidase and thioredoxin that may function in degradation of proteins containing cysteine residues, and changing theredox state of proteins (reductio n of ... gene expression alterations imply carbon meta-bolism imbalance, and indicate higher glucose oversucrose content in cells.As there were clear changes in expre ssion of genesencoding proteins...

Báo cáo y học: " Selective gene silencing by viral delivery of short hairpin RNA" ppsx

... Weinberg RA: The hallmarks of cancer. Cell 2000, 100:57-70.25. Ellis LM, Hicklin DJ: Resistance to Targeted Therapies: Refining AnticancerTherapy in the Era of Molecular Oncology. Clin Cancer ... fanning interest in using RNAi fortherapeutic applications and cancer therapy. A number of publications followed, using retroviral vectors based on Murine Leukemia Virus (MLV) as transfer vehiclesfor ... Ateach end of the genome, long terminal repeats (LTRs)contain promoter/enhancer regions and sequencesinvolved in integration. In addition there are sequencesrequired for packaging the viral...

báo cáo khoa học: " Comparative gene expression profiles between heterotic and non-heterotic hybrids of tetraploid Medicago sativa" pps

... quantitativelyinherited traits like yield – results from the complexactions of many components, including the timing of the expression of various genes, the magnitude and location of their expression, and ... result of a number of gene interactions, and hence, the regulation of expression of any single gene may also have a complex genetic basis.This complexity shows that the genetic control of quanti-tative ... levels of interactions, from genes to gene expression profiles toproteins and metabolites, occur to produce the ultimatephenotype.Conclusion Gene expression profiles between two heterotic...

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