báo cáo khoa học: " Pyrosequencing, a method approved to detect the two major EGFR mutations for anti EGFR therapy in NSCLC" ppt

... 5’-CATGTGGCACCATCTCACAAT-3’ 62°C 5’-GAATTCGGATGCAGAGCTTCTT-3’ 61°C PCR 5’-Biotin-CCCACA CAGCAA AGCAGAAACT-3’ 5’-Biotin-CTTTCTCTTCCGCACCCA primer for sequence reaction 5’-TAAAATTCCCGTCGC-3’ 5’-CATGTCAAGACTACAGATT-3’ Dufort ... mutation account for approximately 90% of EGFR mutations reported to date in lung adeno- carcinoma [3]. The class I mutations are in- frame dele- tions...

Ngày tải lên: 10/08/2014, 10:21

... adapted to a 1536-well plate format. In addition, as the SELEX c an be run simulta- neously against a mixture of targets and the AlphaSc- reen ® analysis can be carried out against individual targets ... capture capacities under the current assay conditions. Therefore the anchor-based methodology allows for monitoring aptamer-target interactions. We then used such a strate...

Ngày tải lên: 11/08/2014, 00:23

... structural factors in disambiguatiou can be taken into account and a lot of data about disambiguation preferences can be explained without any further machin- ery. The Scope Constraint, for example, ... lexical items. In this way, the role of semantic factors in interpretation can be taken into account; in addition, a semantic value can be assigned to a representatio...

Ngày tải lên: 20/02/2014, 21:20

... is that one was calculated based on the ASR compo- nent output being translated to the other language, while the other was calculated from human tran- scribed text being translated to the other language. Table ... that the patient had been injured in a fall down a stairway, the fact that the patient was experiencing blurred vision, and so on. Significant facts did not...

Ngày tải lên: 08/03/2014, 04:22

... that NtKTI1 exerted prominent antifungal activity towards R. solani and moderate antifungal activity against Rhizopus nigricans and Phytophthora parasitica var. nicoti- anae. Bioassays of transgenic ... antimicrobial assay and in planta studies demonstrated that NtKTI1 is an antifungal protein that increases the resistance of tobacco to fungal attack. Results Isolation and characterizat...

Ngày tải lên: 23/03/2014, 03:20

... of fat mass remains to be determined. Available epidemiological data addressing the impli- cation of PAH, in general, as a causal factor in the pathogeny of metabolic disorders are currently too few ... intake, caused by the lack of a satiety signalling, whereas no change in food intake was detected in the B [a] P-treated animals. In addition, the absolute values of...

Ngày tải lên: 30/03/2014, 11:20

... surface realizer (Kow, 2007), which uses a lexicalized feature-based Tree Adjoining Grammar to generate all possible para- phrases for a given flat semantic input. GenI im- plements an optimization ... computational complexity that was originally solved by the pipeline model. Strategies that have been introduced to reduce the search space in integrated systems include greedy/in...

Ngày tải lên: 31/03/2014, 00:20

... issues surrounding the application of the decision aid and act as a sounding board for initial feedback and refinement of the draft decision aid. Finally, the Delphi method will be used to further inform and ... narrative account tracing the historical development of concepts, theory, and methods within each research tradition, referred to as meta-narratives. In synthesi...

Ngày tải lên: 10/08/2014, 10:23

... essoria of M. oryzae, although these appeared small and thin with electron microscopy [25]. Therefore, the formation of papillae a ppears to be a general form of basal defense against attempted appressoria ... construction A 298 bp partial fragment of HvCEBiP was amplified by primer pairs HvCBP1-S1 (5’ -CCAAAGACCCTCAA- GAAGGA-3’)andHvCBP1-AS1(5’-AGCCGTTGGAA- TAACCACTG-3’ )fromcDNAof...

Ngày tải lên: 11/08/2014, 11:21

... our analysis to other ASR systems in order to determine the generality of our findings, we have already gained important insights into a number of factors that increase ASR error rates. In addition, ... mean values, making parameter estimation in the combined model difficult. Prelimi- nary investigation indicated that using the mean val- ues would lead to the best overall...

Ngày tải lên: 20/02/2014, 09:20