báo cáo khoa học: " Activation of the hedgehog pathway in chronic myelogeneous leukemia patients" ppt

... Access Activation of the hedgehog pathway in chronic myelogeneous leukemia patients Bing Long, Huanling Zhu * , Cuixia Zhu, Ting Liu, Wentong Meng Abstract Background: Hedgehog (Hh) signaling pathway ... of the Hh pathway maybe associated with CML progression. Treatment of CML with imatinib, a selective inhibitor of the BCR-ABL tyrosine kinase inhibitor, has...

Ngày tải lên: 10/08/2014, 10:20

... mutant receptors on the binding of acetylcholine The binding properties of ACh were investigated in intact Xenopus oocytes expressing WT and mutant nAChR. The affinity of the mutant receptors for ... its inhibition of the initial rate of 125 I-labelled a-BgTx binding to Torpedo nAChR expressed on the surface of oocytes (Fig. 5). ACh inhibited the initial rate of 1...

Ngày tải lên: 19/02/2014, 07:20

... comparison of blots, it can be seen that most of the acetylated tubulin and ATPase were involved in the immunoprecipitation process. By measuring the intensity of the ATPase band in Fig. 5B (lane ... reflects for- mation of the tubulin–ATPase complex. The HAT quantity increased as the tubulin concentration increased. Taken together, these results indicate that the a...

Ngày tải lên: 23/03/2014, 11:20

... the presence of a minor population of AQP-2-containing vesicles. Taken together, we suggest the biological relevance of our findings as follows. The binding of AVP to V 2 receptors on the renal collecting ... fractionated close to the top of the gradient, suggesting the low-density nature of the AQP-2-containing vesicles. On the other hand, P-LAP fractionated diff...

Ngày tải lên: 08/03/2014, 02:20

... both the N-terminal and C-terminal domains of hoPL were involved in the inactivation of the protein at the water–triglyceride interface. The inactivation of N2Cc in the absence of bile salts was ... In conclusion, the accessi- bility of the active site was better in the protein bear- ing the N2 domain than in the protein bearing the Nc domain. Thus,...

Ngày tải lên: 16/03/2014, 06:20

... 17 residues of 4E-BP1 contain the RAIP motif. To assess whether removal of the RAIP motif accounts for the reduced binding of raptor to the 18– 117 fragment, we compared the binding of raptor to this ... after treatment of cells with insulin [25]. Replacing its N-terminus with the N-terminal part of 4E-BP1 led to a marked increase in its phosphoryla- tion (even in...

Ngày tải lên: 16/03/2014, 06:20

... to the in uence of the remaining free amino acids in the hydrolysate. HOX, GOOX comparison According to the model of FAD in HOX presented in this article, the surroundings of the coenzyme in the two ... [7]. These enzymes are typ- ically composed of two major domains, one of which binds the FAD (the F-domain) and one of which binds the substrate (the S...

Ngày tải lên: 16/03/2014, 14:20

... membrane and in the extracellular space. The weak staining of extracellular material is not surprising given that detergents are included in the staining pro- tocol. Thus, the staining for PtdIns(3,4,5)P 3 is ... the SEC14 domain of PTP- MEG2 was shown to bind PtdIns(3,4,5)P 3 in vitro and colocalized with it in intact cells [5]. We therefore deci- ded to study the bin...

Ngày tải lên: 16/03/2014, 23:20

... demonstrating that, despite extensive mutagenesis in the vicinity of the FAD-binding pocket, the mutants remain properly fla- vinylated (not shown). The thermal stability was inves- tigated using the ThermoFAD ... mm sodium dithionite in the absence of oxygen, and recording of the spectra of the reduced states was repeated. To investi- gate the effect of the m...

Ngày tải lên: 29/03/2014, 09:20

... transformation of the SF1 strain with a plasmid carrying carB36. In five of them, Southern blot analyses showed the incorporation of a single copy of the plasmid at the carB locus (Fig. 4A,B). Three of these ... Physical map of the pB21H integration at the homologous carB sequence by a single recombination event in the genome of strain SF1. The mutation in...

Ngày tải lên: 30/03/2014, 01:20