... 1Protein kinase, putative 1Ras-related GTP-binding protein, putative 1RNA-binding protein 5, putative 1RNA-binding protein, putative 1Serine acetyltransferase, putative 1Serine ⁄ threonine ... contain various proteins in additionto TcDHH1, including heat shock proteins, mRNA-binding proteins, initiation and elongation translationfactors, ribosomal proteins and metabolic proteins.Abundant ... was incubatedwith resin containing protein A Sepharose (Sigma) for16 h at 4 °C with agitation. Antiserum (150 lL) wasmixed with 150 lL of resin and 1 lL of RNAse OUT(Invitrogen). After incubation,...