Báo cáo y học: "Intrapleural instillation of autologous blood for persistent air leak in spontaneous pneumothoraxis it as effective as it is safe" pps

... STU D Y PRO T O C O L Open Access Intrapleural instillation of autologous blood for persistent air leak in spontaneous pneumothorax- is it as effective as it is safe? Dimos Karangelis * , Georgios ... patients of high morbidity and mortality surgical risks who are ineligible for surgery. Conclusively blood pleurodesis is an “only-win” method as th...

Ngày tải lên: 10/08/2014, 09:22

... Japan. 5 Laboratory for Systems Biology and Medicine, RCAST, The University of Tokyo, Tokyo, Japan. 6 Dept. of Biophysics and Biochemistry, Osaka University, Graduate School of Medicine, Suita, Japan. 7 Medical ... immunohistochemically except for 2 cases w ith faintly positive for Syn but histopathological differentiation from SC, AC and SCLC was required for its definite diag-...

Ngày tải lên: 10/08/2014, 09:22

... primary goal of ACS treatment is alleviation of OA symptoms, one of the mechanisms may be enhancement of cartilage integrity through the inhibition of inflammatory activity, in particular with ... bioactivity of most cytokines, while their immunoreactivity as determined by ELISA is still retained [38]. In particular Il-10, one of the anti-inflam- matory cytokines upreg...

Ngày tải lên: 12/08/2014, 14:22

... both in total number and in maturity, within the thymus. The increase in B cell numbers in the thymus with age seems too small to account for the greatly increased quantities of immunoglobulin ... 3. Protein levels of immunoglobulin increase within the thymocytes with age. Western blot analysis of IgM heavy chain protein levels with successive age. This image is represent...

Ngày tải lên: 03/11/2012, 11:35

... Enthalpy and heat capacity changes for formation of an oligomeric DNA duplex: interpretation in terms of coupled pro- cesses of formation and association of single-stranded helices. Biochemistry 38, ... Moreover, the development of DNA chips for rapidly screening and sequencing unknown DNAs mainly relies on the ability to predict the thermodynamic stability of the complexes...

Ngày tải lên: 22/02/2014, 07:20

... the Netherlands) in order to obtain a recombinant protein containing the C-myc epitope followed by six histidines at the C-terminus. A plasmid containing the wild type version of CCR5 was constructed by introducing ... were obtained with CCR5-myc-his protein Fig. 3. CCR5-myc-his purification. CCR5-myc-his was purified by immobilized metal ion affinity (A) or immunoaffinity chromatography using...

Ngày tải lên: 08/03/2014, 09:20

... machinery towards the synthesis of s ome enzymes involved in glycolysis or sugar-phosphate metabolism [54]. HydA belongs to a new class of Fe-hydrogenases HydA of C. reinhardtii, the first isolated ... vol. of fresh TAP medium and th e algae were a naerobically adapted b y flushing the s olution withargoninthedark. Hydrogen evolution assay Hydrogenase activity of C. reinhardtii wa...

Ngày tải lên: 08/03/2014, 22:20

... C a of Glu and its carboxylate oxygen, C a of Asp a nd its carboxylate oxygen, C a of Arg and its side-chain amines or guanidinium, and C a of Lys and its side-chain amine was determined by measuring ... he N-terminal amine of t he P1 residue with a c arboxylate oxygen of Asp273 (Asp366), the carbonyl oxygen of the P1 residue with the side-chain amine of Lys262 (Lys354), and t...

Ngày tải lên: 08/03/2014, 23:20

... unchanged in contrast to the highly increased affinity for ATP [36]. Similar findings were made by uncoupling E. coli cells expressing the rat AACs with CCCP. The affinity for ADP of both rat AACs was ... Germany). T7- RNA polymerase synthesis was induced by adding 1 m M IPTG. After shaking the culture for 15 min at 37 °C, 20 lL rifampicin (stock 20 mgÆmL )1 , dissolved in methanol)...

Ngày tải lên: 18/03/2014, 01:20

... of cofactor for TDP enzymatic activity was examined by determining the specific activity of TDP by following the cleavage of 1 m M of substrate by 0.125 l M of enzyme in reaction buffer containing ... that this assay has a sensitivity concentration as low as 15 n M , which is comparable with the sensitivity of the gel-based assay [14]. Kinetic parameters K m , k cat and V...

Ngày tải lên: 31/03/2014, 21:21