Báo cáo y học: "Quantitative analysis of histone exchange for transcriptionally active chromatin" pdf

... presence of an equivalent amount of iso- topically heavy histones. The exchange of histones (i.e., the incorporation of isotopically heavy histones during the isolation of isotopically light histones) ... 30:249-262. doi:10.1186/2043-9113-1-17 Cite this article as: Byrum et al.: Quantitative analysis of histone exchange for transcriptionally active chromatin. Journal...

Ngày tải lên: 10/08/2014, 09:22

... superiority of the CDU process in terms of cleaning efficacy at repro- cessing more complex instruments. Previous studies have focused on the efficacy of CDU reprocessing by assaying a range of surgical ... ensitivity have emerged. Examples include a fluorescent microscopy technique involving visualisation of protein by SYPRO ruby staining capable of detecting 85 pg of p rotein...

Ngày tải lên: 10/08/2014, 21:24

... ms. For the purposes of univariate analyses, the patients were stratified into two groups based on the QTc interval, with a cutoff line of 470 ms (Table 1). Univariate analysis Univariate analyses ... acute phase of SAH [1,6]. However, one cannot refute the possibility that the catecholamine surge may influence the QT interval indirectly via hypokalemia, because hypokalemia (as well...

Ngày tải lên: 12/08/2014, 19:22

... microarrays; GSEA: gene set enrichment analysis; H3K4 me3: histone H3 trimethyl-lysine 4; H3K27 me3: histone H3 trimethyl-lysine 27; HIF-1: Hypoxia-inducible factor-1; MAT: Model-based Analysis of Tiling-array; ... acutely subjected to hypoxia, HIF-1 preferentially bound to loci that were already transcriptionally active under normal growth conditions characterized by the presenc...

Ngày tải lên: 09/08/2014, 20:20

... analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase ... healthy control donors. The message expression of hyaluronan synthase-1 and -2 in the synovium of both types of arthritis was significantly less than in the control synovium, wher...

Ngày tải lên: 09/08/2014, 01:24

... for analysis. The intracellular location of the Bag-1 Morpho/AS was confirmed by fluorescent microscopy. Cell viability assay Cell viability assays were performed after 12 and 24 hours of ... used for the PCR were as follows: 94°C for 2 min, 30 cycles of 94°C for 45 sec, 68°C for 45 sec, and 74°C for 1 min, with final extension at 74°C for 3 min. The integrity of...

Ngày tải lên: 31/10/2012, 15:12

... of hCDC4 can also be observed in Acute Myeloid Leukemia (AML) or high risk Myelodysplastic Syndrome (MDS). Therefore we carried out a mutational analysis of the hCDC4 gene in 35 samples of ... Sequence analysis of hCDC4 exon 10 identifies a new intronic SNP During the analysis of exon 10 and adjacent intronic sequences we could for the first time identify a new sing...

Ngày tải lên: 31/10/2012, 16:49

... levels of many of these genes identified in the array analysis. Moreover, Western blot analysis of thymo- cyte lysates also confirmed that IgM increases with age (Figure 3). Flow cytometry analysis ... and array analysis. For each array sample, the RNA was prepared from the purified thymocytes. The thymocytes were processed by using the RNEasy Mini Kit (Qiagen, Valencia, CA...

Ngày tải lên: 03/11/2012, 11:35

... concen- tration of DCMU would inhibit completely the activity of PSII by preventing the reduction of the PQ pool [21,22], drastically channging the typical FI trace of thylakoids by eliminating ... further analysis of the influence of PQ reduction on FI was performed after the addition of dPQ to the thylakoid samples. Treatment of thylakoids with 1 lm dPQ had no effect on E A...

Ngày tải lên: 19/02/2014, 05:20

... as marker. Chemical deglycosylation DM64 was chemically deglycosylated with anhydrous trifluoromethanesulfonic acid (TFMS) using the GlycoFree kit k-500 from Oxford GlycoSystems, USA and submitted to ... potentiated by enzymatic degradation of phospholipids [7,44]. The capacity of DM64 to inhibit the myotoxicity induced by myotoxins I (Asp49) and II (Lys49) from B. asper venom was analyzed...

Ngày tải lên: 21/02/2014, 01:21