... treatment of 6e and 7a with trimeth-ylsilyl bromide and methanolysis. Finally, the ethyl estergroup of 8a was hydrolysed under basic conditions, and theZ-andE-isomers were separated by anion-exchangechromatography. ... between 4f and trimethyl phosphite. Therefore 2b and 2d,aswellas2i were prepared by alkaline hydrolysis of the esters 2a, 2c and 2h, respectively. However, 2a wasstable to hydrolysis at pH 12 and the ... inhibitor of PEPmutase [36], and pyruvate kinase [6].Inhibition of pyruvate kinase. This enzyme catalyses theregeneration of ATP from ADP and PEP, in the last step of glycolysis. Due to its physiological...