Báo cáo y học: "Clock mutation affects circadian regulation of circulating blood cells" pot

... study, the circadian rhythm of circulating neutrophils was a damped bimodal process in Clock mutant mice, although that of lymphocytes was only phase-delayed. Granulocytes emigrate from the bloodstream ... Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan, 4 Department of Hygiene and Public Health, School of Human Sciences, Waseda University, 2-5...

Ngày tải lên: 10/08/2014, 09:20

... CFU Functionality of the cultured CPCs was determined in different ways, that is, CFU potential, migratory capacity and cluster for- mation capacity on Matrigel. After seven days of culture, cen- tral ... http://arthritis-research.com/content/9/4/R84 Page 5 of 10 (page number not for citation purposes) Figure 2 Adhesion assay and number and morphology of colony forming units (CFU)Adhe...

Ngày tải lên: 09/08/2014, 10:20

... control of dendritic cell physiology may be selectively influenced by the circadian system. This implies caution in the moni- toring of these immunoregulatory effects which may sub- stantially fluctuate ... time": circadian characteriza- tion of galectin-1 null mice Journal of Circadian Rhythms 2010, 8:4 Casiraghi et al. Journal of Circadian Rhythms 2010, 8:4 http://www...

Ngày tải lên: 10/08/2014, 09:20

... Wajant 1 1 Institute of Cell Biology and Immunology and 2 Institute of Industrial Genetics, University of Stuttgart, Germany; 3 Institute of Immunology, Christian-Albrechts-University of Kiel, Germany To gain ... domain(s) of these TRAF4 variants lead to a reduced affinity–avidity of respective heteromeric com- plexes [T4(259–387)–YFP–T4(259–470), T4(1–307) –YFP– Fig. 7. Transloc...

Ngày tải lên: 21/02/2014, 15:20

... ankylosing spondylitis; BASFI = Bath Ankylosing Spondylitis Functional Index; BASMI = Bath Ankylosing Spondylitis Metrology Index; CMV = cytomegalovirus; EBV = Epstein–Barr virus; ESR = erythrocyte ... metrology score, but they are independent of age and duration of ankylosing spondylitis. CD4 + CD28 – T cells produce IFN-γ and perforin, and thus they must be considered proinflammatory an...

Ngày tải lên: 09/08/2014, 01:23

... monocyte purity and lym- phocyte purity were assessed by flow cytometry, and fractions were typically >80% and 90% pure, respectively. T-cell stimulation and fixation Elutriation-enriched lymphocytes ... 144:3347-3353. 13. Morita Y, Yamamura M, Kawashima M, Harada S, Tsuji K, Shibuya K, Maruyama K, Makino H: Flow cytometric single-cell analysis of cytokine production by CD4 + T cells in...

Ngày tải lên: 09/08/2014, 08:23

... analysis of carboxyfluorescein succinimidyl ester (CFSE)-labeled cells. The authors state that functional analysis of proliferation may provide a better tool for identification of specific monocyte subsets ... rise to osteoclasts in vitro based on an analysis of three healthy controls [10]. In the recent study [1], they analyzed monocytes from 13 healthy donors and demon- strated that...

Ngày tải lên: 09/08/2014, 14:21

... An inhibitor of Akt phosphorylation, LY294002 (10 μM, LY) was pretreated and the level of FMRP was determined by Western blot. (D) After LY treatment, cell viability was measured by MTT assay, inhibition ... inhibition of Akt phosphorylation further decreased cell viability. (E) The level of BcL-xL after LY treatment. Increased BcL-xL expression induced by etoposide treatment was prev...

Ngày tải lên: 10/08/2014, 05:21

... This lack of consensus illustrates the limita- tions of in vitro systems, especially with respect to the availa- bility of suitable in vitro model systems for the study of diseases of articular ... of ank may have subtle effects on chondrocyte maturation. Although this is the first report of a gain -of- function in one of the familial chondrocalcinosis-causing mutations, as pr...

Ngày tải lên: 09/08/2014, 08:23

... homo- zygosity of deleterious mutations may explain the repeated success of asexuals in nature [32]. Whereas any new insertions in a sexually recombining genome can become homozygous, asexuals carry only ... rate of homozygos- ity (also approximately 25%) at these loci a s well. T his elevated risk of homozygosity in sexuals has two major consequences. The first is the potentially lar...

Ngày tải lên: 09/08/2014, 20:21