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Báo cáo y học: " Effectiveness of antiretroviral therapy and development of drug resistance in HIV-1 infected patients in Mombasa, Kenya" doc

Báo cáo y học:

Báo cáo y học: " Effectiveness of antiretroviral therapy and development of drug resistance in HIV-1 infected patients in Mombasa, Kenya" doc

... Research and Therapy Open AccessShort report Effectiveness of antiretroviral therapy and development of drug resistance in HIV-1 infected patients in Mombasa, KenyaKim Steegen1,2,3,6, Stanley Luchters1,2, ... and genotyping of HIV RNA wasperformed by nested RT-PCR and an in- house sequencingassay as described elsewhere [4]. The interpretations of genotyping data and subtyping were performed usingSmartgene™ ... Kenya AIDS Indicator Survey 2007. Nairobi Kenya: NationalAIDS and STI Control Programme, Ministry of Health Kenya(NASCOP); 2008. 2. Guidelines for Antiretroviral Drug Therapy in Kenya. 3rdedition....
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Báo cáo y học: "Pravastatin Provides Antioxidant Activity and Protection of Erythrocytes Loaded Primaqe"

... pravastatin and primaquine exposed eryt-hrocytes. All groups of erythrocytes were fixed in buffered gluteraldehyde. Aldehyde was drained and rinsed 3 times each of 5 min in phosphate buffer and ... in the current studies upon loading to erythrocytes. The erythrocytes were classified into; control erythrocytes, erythrocytes incubated with either 2 mM of PS or 2 mM of PQ, and erythrocytes ... susceptibility of erythrocytes to hemolysis in presence of primaquine was greater than that of control in ad-dition to pravastatin treated erythrocytes. This find-ing are similar to the previous study...
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Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot

Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot

... hydrolysis by  70% whereasATP hydrolysis was inhibited by only 10%. Inhibition of ADP and ATP hydrolysis by azide was greater when the pH of assay solutions was 6.4. Azide inhibition was diminishedif ... Enzyme assays were carried out in 0.25-mLreaction mixtures using a wide-range buffer system (piperazinedihydrochloride/glycylglycine/NaOH [31]), covering the pH range of 4.6–9.5. The assay solutions ... obtained byimmunoaffinity chromatography with a  50-fold purifica-tion in one single step. The significant loss of total activityprobably resulted from irreversible binding of the majorityof...
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Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx

Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx

... usuallyprecedes the guanine nucleotide-binding NKXD motif byalanine and replacement of a conserved phenylalanine 10residues C-terminal to the EXS[AV] motif by alanine,threonine, serine or ... Blue-stained gel showing 10 lg of platelet protein and 0.5 lg of GST and GST±Rab p roteins; (B) an[a-32P]GTP overlay [17] of a nitrocellulose blot of a gel containing40 lg of platelet protein, ... lgofGSTand0.5lgofGST±Rabproteins, except for GST±Rab5A (0.1 lg); (C) an immunoblot of a gelcontaining 20 lgofplateletproteinand0.1lg of GST and GST±Rabproteins, using a 1 : 1000 dilution of...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc

Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc

... in heterodimerization of the intracellular domains of b and ccchains followed by a ssociation with Jak, Syk (or src family)kinases. These, in turn, phosphorylate the receptor c hains,forming docking sites ... Simons, K. (1999) Clusters of glycolipid and glycosylphosphatidyl-inositol-anchored proteins in lymphoid cells:accumulation of actin regulated by local tyrosine phosphorylation.Eur. J. Immunol. ... stimulated tyrosine-phosphorylation of STATsIL-2 induced tyrosine phosphorylation of STAT3 (and STAT5) was followed by flow cytometry as described previ-ously for STAT1 [32]. Brie y, cells with...
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Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps

... risk of triggering animmune response finally culminating in synovitis. However,genetic susceptibility factors of the host are also playing animportant role particularly in persistent infections ... RA patients and in three of six(50%) OA patients. Accordingly, the presence of bacterialDNA within the SF samples was significantly higher in ReA and UA patients as compared with RA and OA patients ... composition of bacterial DNA may changeover time in joint cavity.Accordingly, the analysis of SF or ST samples from differentarthropathies patients by broad-range PCR is essentiallycapable of characterising...
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Báo cáo y học: "MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays" pptx

... DNAmethylation indirectly. However, they differ in the way theyinterrogate the amount of DNA methylation, leading to spe-cific experimental constraints that limit the application of each method ... classify the training samples into cases and controls,using hierarchical clustering of the DNA methylation profiles.Consistent with previous observations, we obtain a large clus-ter of samples in ... translating basic research findingsinto the clinic is partially due to a discontinuity of methodsbetween the discovery phase and the validation phase. Themethods used most commonly in the discovery...
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Báo cáo y học: "Quake: quality-aware detection and correction of sequencing errors" docx

... confound ana-lyzes by introducing false polymorphisms and fragment-ing genome assemblies. The Quake system detects and corrects sequencing errors by using the redundancyinherent in the sequence ... foradenine by quality value for a single lane of Illumina sequencing of Megachile rotundata. The number of training instances at eachquality value are drawn as a histogram below the plot. At low and medium ... miscall rates bysequencing reads from Helicobacter acinonychis and Betavulgaris, aligning them to high quality reference gen-omes, and counting the number of each type of mis-match in the alignments...
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Báo cáo y học: "Comparative genomics reveals birth and death of fragile regions in mammalian evolutio" pdf

... Ferguson-Smith M, Stanyon R, Yang F, Graphodatsky A: Cross-species chromosomepainting in Cetartiodactyla: reconstructing the karyotype evolution in key phylogenetic lineages. Chromosome Research ... distant pairs of branches (H+ and M+, H+ and R+,Q+andM+, Q+andR+ in the yellow cells) feature thelowest inter-reuse. The only branch that shows relativelysimilar inter-reuse (varying from 58 ... = m)sincethey do not af fect m-ninactive breakpoints in FBM.To distinguish FBM from RBM, one has to analyze thelong cycles in the breakpoint graph and the distribution of synteny block sizes...
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Báo cáo y học: "Vive la différence: biogenesis and evolution of microRNAs in plants and animals" ppt

... their 2′-O-methylation to remain stable in the presence of highly complementary targets [102]. Reciprocally, instability of Drosophila miRNAs loaded in AGO1 was induced by providing them an artificial, ... heterogeneous small interfering RNAs (siRNAs). As regulatory relationships are rened, the precision and phasing of hairpin processing may increase. Shortening of the hairpin to produce a single dened ... are methylated by HEN1 as single-stranded species [39-42]. In addition to this core machinery, several accessory factors in uence the biogenesis efficiency, fidelity and sorting of animal miRNAs...
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