Báo cáo y học: "Immunomodulatory properties of human adult and fetal multipotent mesenchymal stem " pptx

... between human fetal liver and adult bone marrow mesenchymal stem cells. Haematolog- ica 2005, 90:1017-1026. 20. Le Blanc K, Tammik L, Sundberg B, Haynesworth SE, Ringden O: Mesenchymal stem cells ... Characterization and functionality of cell surface molecules on human mesenchymal stem cells. J Biomed Sci 2003, 10:228-241. 71. Deans RJ, Moseley AB: Mesenchymal ste...

Ngày tải lên: 09/08/2014, 08:23

... poly(ethylene glycol) by the addition of 0.67 vol. of 30% poly(ethylene glycol) in buffer A, and after 10 min centrifuged at 10 000 g for 20 min. The resulting pellet was resuspended in 50 mL of ... control by metabolic intermediates of the pathway of sucrose synthesis; 6PF2K activity is stimulated by Fru-6-P and P i , and inhibited by three-carbon phosphate esters (including...

Ngày tải lên: 22/02/2014, 04:20

... phosphorylation of Ser16, analysis of their oligomeric state, chaperone activity and their ability to interact with human Hsp27. Materials and methods Proteins Cloning and mutagenesis of human ... presence of 1-(3- dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) (5 m M )andN-hydroxysuccinimide (NHS) (5 m M ). The reaction was stopped by the addition of SDS sam...

Ngày tải lên: 07/03/2014, 15:20

... puri®cation of CBG and allowed characterization in s ome detail, especially with respect to its glycone/aglycone speci®city a nd ability to catalyse the hydrolysis of dietary xenobiotic glycosides. ... hibitor of reCBG [the hydrolysis of 4NP-b- D - glucopyranoside (10 m M ) was reduced by 98% in the presence of 4NP-b- D -mannopyranoside (10 m M )]. Hydrolysis of xenobiotic glyco...

Ngày tải lên: 08/03/2014, 16:20

... atoms: C t of the C-terminus, C c of Asp and C d of Glu, and O g of Tyr. Positive unit charges were added at the following atoms: N t of the N-terminus, N f of Lys, C f of Arg, and N d or N e of His ... highly charged and has not been the subject of detailed study. The hydrophobic and charged faces of the RIIa D/D are diametrically opposed to each other. Interest...

Ngày tải lên: 08/03/2014, 22:20

... some commercially available proteins, which include human serum albumin, cytochrome C, lysozyme, alcohol dehy- drogenase, b-amylase and carbonate anhydrase, were used in the activity assays. The substrate ... PB2-T2 enzyme exhibited activity that was only 17% of that of PB2-T1. These results show that the refolded and purified pro-BACE2 enzymes (PB2-T1 and PB2-T2) are active in hyd...

Ngày tải lên: 17/03/2014, 10:20

... The requirement of cofactor for TDP enzymatic activity was examined by determining the specific activity of TDP by following the cleavage of 1 m M of substrate by 0.125 l M of enzyme in reaction ... the hydrolysis of substrate by water, by heating the enzyme at 70 °C for 15 min prior to the assay. The enzyme reactions carried out with heated Fig. 1. Sequence alignment of human...

Ngày tải lên: 31/03/2014, 21:21

... high- and low-intensity fractions by cell sorting, and they found that the suppressive effects were only displayed by the high-intensity fraction [17]. This subset did not produce cytokines. Cytokine-dependent ... extremely potent suppressive activity. These CD4 + cells had the phenotype and functional characteris- tics of ‘professional’ regulatory T cells. Using the genera- tion cyto...

Ngày tải lên: 09/08/2014, 03:24

... induced by Th1 cells was not significantly affected by any of the inhibitors used, but the MCP-1 levels induced by Th2 cells were affected strongly by both U-0126 and PSI and weakly by the JNK ... IL-8 was not induced by any of the Th1 and Th2 cytokines, but was strongly induced by Th2 and to a lesser extent by Th1 cells – an effect due to cytokines (IL-1 and TNF-α) shared by th...

Ngày tải lên: 09/08/2014, 07:20

... ultrasound system, typical ultrasound echo and wavelet mapThe ultrasound system, typical ultrasound echo and wavelet map. (a) The ultrasound measurement system employed, consisting of a transducer, ... Nakamura 2 1 Department of Physical Therapy, Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 Kawahara-Cho, Shogoin, Sakyo-Ku, Kyoto 606-8507, Japan 2 Dep...

Ngày tải lên: 09/08/2014, 10:23