Báo cáo y học: "MiR-204 regulates cardiomyocyte autophagy induced by ischemia-reperfusion through LC3-II" ppt

... rowth-inhibitory eect was also attenuated by this modi®cation. These results suggest that growth inhibition by ECP is dependent on cell type and is cytostatic. Keywords: cell cycle; colony formation; cytostatic ... ied by the colonies was evaluated by image scanning assisted by a computer. Fluorescence microscopy ECP and bovine RNase A were labeled with rhodamine B isothiocyanate (...

Ngày tải lên: 17/03/2014, 17:20

... a hypoth- esis by which prolonged CI could worsen A V by increasing the early innate inflammatory response and consequently augmenting the adaptive immune response [22]. We explored this hypothesis ... ARTIC L E Open Access Early innate immune events induced by prolonged cold ischemia exacerbate allograft vasculopathy Jennifer J Devitt 1* , Chelsey L King 2 , Timothy DG Lee 1,2,3 , Ca...

Ngày tải lên: 10/08/2014, 09:23

... Sci 2011, 18:22. 28. Jian X, Xiao-yan Z, Bin H, Yu-feng Z, Bo K, Zhi-nong W, Xin N: MiR-204 regulate cardiomyocyte autophagy induced by hypoxia-reoxygenation through LC3-II. Int J Cardiol 2011, ... of 5 RESEARCH Open Access MiR-204 regulates cardiomyocyte autophagy induced by ischemia-reperfusion through LC3-II Jian Xiao 1† , Xiaoyan Zhu 2† , Bin He 3† , Yufeng Zhang 1...

Ngày tải lên: 10/08/2014, 05:21

... strains. Discussion RA histology is typically characterized by pronounced synovial hyperplasia, also called 'pannus'. The RA pannus produces proinflammatory cytokines and proteases, and ... of primary FLS FLSs were isolated by enzymatic digestion of the synovial tis- sue. Briefly, tissues were minced and incubated with a solution containing DNase 0.15 mg/ml, hyaluronidase type I...

Ngày tải lên: 09/08/2014, 10:23

... p a i r pathway initiated by poly(ADPribose)polymerase and the DNA repair pathways initiated by ATM/ATR are necessary for efficient apoptosis resulting from NS1 expression. Key words: Parvovirus ... damage repair pathways, mediated by ATM or ATR, are ne- cessary for optimal induction of apoptosis induced by NS1 expression, since inhibition of these pathways significantly decre...

Ngày tải lên: 25/10/2012, 11:18

... “Click Chemistry” introduced by Shar- pless and which can be considered as a chemical phi- losophy.[7] As described by Finn and Fokin: the ‘‘Click’’ moniker is meant to signify that by use of these ... possible with variable chemistry: (I) either in a cleavable form by a reversible disulfide bridge bond or (II) by non-cleavable covalent bonds and a (III) by hydrogen bridge fo...

Ngày tải lên: 26/10/2012, 08:57

... Chang, C., Eom, S.H., Hwang, K .Y. & Cho, Y. , Yu, Y. G., Ryu, S.E., Kwon, S.T. & Suh, S.W. (2000) Crystallization and preliminary X-ray crystallographic analysis of NAD + -dependent DNA ligase ... Hakansson,K.,Doherty,A.J.,Shuman,S.&Wigley,D.B.(1997) X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes. Cell 89,545– 553. 3...

Ngày tải lên: 21/02/2014, 01:21

... temporal sequence by a variety of mechanisms [12,71,82]. Stimulation of these pathways induces cardiac hypertrophy by promo- ting individually or synergistically protein synthesis and immediate early gene ... signalling may induce apoptosis [61] and alterations in this pathway are an underlying cause of cardiac toxicity. Apparently, in adult mouse cardiomyocytes, the apoptosis induced...

Ngày tải lên: 21/02/2014, 15:20

... used: 1 cycle: 30 min, 50 °C; 1 cycle: 2 min, 94 °C; 35 cycles: 30 s, 94 °C, 30 s, 55 °C; 1min,72°C, followed by 1 cycle for 10 min at 72 °C. For the detection of b-actin, the number of cycles was ... cycle synchronization was monitored by FACScan analysis of parallel samples (Fig. 5B, bottom). E2F6 and E2F6b mRNA levels were lowest in serum-starved cells. During re-entry into the cell cy...

Ngày tải lên: 08/03/2014, 09:20

... mixtures of methyl glycosides were analyzed on GLC [14,15]. The absolute configurations of the monosac- charides were determined by GLC analysis of the trimeth- ylsilylated (–)-2-butyl glycosides [16,17]. ... acid by coevaporation with methanol, the mixture of partially methylated alditols was acetylated with acetic anhydride (3 h, 120 °C), and analyzed by GLC and GLC–EIMS [14,19]. NMR s...

Ngày tải lên: 08/03/2014, 09:20