Báo cáo y học: "RNA interference of argininosuccinate synthetase restores sensitivity to recombinant arginine deiminase (rADI) in resistant cancer cells" pps

... interference of argininosuccinate synthetase restores sensitivity to recombinant arginine deiminase (rADI) in resistant cancer cells Fe-Lin Lin Wu 1,2,3 , Yu-Fen Liang 1 , Yuan-Chen Chang 1 , Hao-Hsin Yo 1 , ... resistance, RNA interference Background Arginine deiminase depletes arginine by hydrolyzing it to ci trulline. Pegylated recombinant arginin...

Ngày tải lên: 10/08/2014, 05:21

... Cell Therapy, Kyoto University Hospital, Kyoto, Japan. 2 Division of Internal Medicine, Kyoto Second Red Cross Hospital, Kyoto, Japan. 3 Department of Molecular Cell Ph ysiology, Kyoto Prefectural ... J, Sato K, Yokota A, Segawa H, Toda Y, Kageyama S, et al: Intravesical administration of small interfering RNA targeting PLK-1 successfully prevents the growth of bladder cancer. J Cli...

Ngày tải lên: 10/08/2014, 09:22

... component of LV systolic duration is increased in pa- tients with dilated cardiomyopathy. A disturbed elec- tromechanical activation leading to intraventricular dyssynchrony is typically present in ... during stress (22). Further- more, exercise LV dyssynchrony may play a more important role in the pathophysiology of left ven- tricular remodelling than baseline LV dyssynchrony...

Ngày tải lên: 05/11/2012, 11:32

... hydrophobicity and Gly, Ser, Asp and His mutants to provide hydrophilicity. The catalytic roles of Cys341 in carboxypeptidase Y are examined by comparing the kinetic parameters of the Cys341 mutant enzymes. MATERIALS ... chloromethyl ketone derivative of benzyloxycarbonyl- L -phenylalanine. J. Biol. Chem. 250, 5221–5226. 18. Bernardi, G. (1971) Chromatography of proteins on hydr...

Ngày tải lên: 08/03/2014, 23:20

... of significant contaminant by nucleotide. Assay of enzyme activity The activity of the shikimate kinase was determined by a double coupled assay involving pyruvate kinase (PK) and lactate dehydrogenase ... [27]. Unfolding and refolding of SK was performed essentially as described in our previous studies on type II dehydroqu- inase [28]. To study the extent of unfolding of SK, t...

Ngày tải lên: 08/03/2014, 23:20

... favorable toward gene therapy. Lupus is thought to involve excess production of type 2 cytokines, so a number of investigators have introduced type 1 cytokines, such as IL-2 and IL-12. Encouraging ... neutralizing immune reaction [21] involv- ing the generation of cytotoxic T lymphocytes [22]. This led to transient transaminitis and curtailed transgene expression. In light of th...

Ngày tải lên: 09/08/2014, 01:22

... with early rheumatoid arthritis (RA), compared to a single baseline determination. Ninety-nine RA patients with disease durations of less than one year and no history of disease-modifying antirheumatic ... responsible for the analysis of the rheumatoid factors. BC was a main clinical investigator, contributed to the preparation of the manuscript, and was involved in all aspects...

Ngày tải lên: 09/08/2014, 07:20

... known as stromelysin-2) is a member of the stromelysin family of enzymes, along with MMP-3 (stromelysin- 1) and MMP-11 (stromelysin-3). This family of enzymes exhibit a wide range of substrate specificities, ... IX [18]. In addition to this proteolytic activity, MMP-10 has been shown to play a role in the activation of a number of other members of the MMP family in a r...

Ngày tải lên: 09/08/2014, 14:22

... Anderson MS, Raetz CR: Regulation of UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase in Escherichia coli. The second enzymatic step of lipid a biosynthesis. J Biol Chem 1996, 271:25898-25905. ... are not in upstream regions but may be physiologically relevant. It may be that there are as yet undiscovered non-coding RNAs present near these binding sites, or that bin...

Ngày tải lên: 09/08/2014, 23:20

... consistency in HIV-1 inhibition for >20 experiments. In addition, in this study the cytotoxicity was carefully defined in cell cycle Table 2: WR1065 did not alter cell cycle parameters in HIV-1-uninfected ... Annexin V positivity (apoptotic) and negativity (non-apoptotic). Flow Cytometry for determination of cell cycle parameters in human TCBs cultured in the presence of...

Ngày tải lên: 10/08/2014, 05:21