Báo cáo y học: " Regulation of cardiac microRNAs by serum response factor" potx

... expression of a number of microRNAs. Interestingly, downregulation of miR-1, miR-133a and upregulation of miR-21 occurred by 7 days of age in these mice, long before the onset of cardiac hypertrophy, ... 18:15 http://www.jbiomedsci.com/content/18/1/15 Page 2 of 13 RESEARCH Open Access Regulation of cardiac microRNAs by serum response factor Xiaomin Zhang, Goha...

Ngày tải lên: 10/08/2014, 05:21

... were analyzed by densitometry using Kodak Digital Science software (Eastman Kodak, Rochester, NY, USA). Data were analyzed by paired t-tests, or by analysis of variance followed by Tukey's multiple ... command of p300, because Sox9 activity was reduced by approximately 50% when NF-κB was activated. However, the demand for p300 is controlled not only by rela- tive affinity...

Ngày tải lên: 09/08/2014, 10:22

... ArgII and ⁄ or polyamine in the regulation of apop- tosis [21,22]. Thus, the possibility that the antiviral effect of spermine is mediated by induction of apop- tosis was analyzed. For this purpose, ... inhibited by pyrrolidine dithio- carbamate [28]. In Raw 264.7 cells cocultured with H. pylori, ArgII expression was inhibited by MG-132 [20], suggesting indirectly an involvement...

Ngày tải lên: 07/03/2014, 21:20

... identification by Bigey et al.ofmany Correspondence to K. K. Yokoyama, Gene Engineering Division, Department of Biological Systems, BioResource Center, Tsukuba Institute, RIKEN (The Institute of Physical ... exclusively but, rather, by a set of different proteins that are structurally related [1]. The promoter regions of many eukaryotic genes contain GC-rich sequences [2] and some...

Ngày tải lên: 22/02/2014, 07:20

... the Prime-a-gene labeling system (Promega). Double-positive clones were purified by secondary and tertiary r ounds of screening and genomic DNA inserts were purified using poly(ethylene glycol) precipitation ... responsible for the hyperacute vascular rejection occurring in pig-to-primates organ trans- plantation. The synthesis of the aGal epitope is catalyzed by the enzyme a1,3-galacto...

Ngày tải lên: 08/03/2014, 16:20

... study down -regulation of the RAS–ERK signalling p athway and other functions of RAS. Down -regulation of the RAS– ERK pathway may be a critical step in the process of end- stage megakaryocyte ... the a bility of TPO to activate the e arly events of the R AS signalling pathway. However, a recent study reported that inhibitors of PtdIns3K abolished potentiation of ERK by TP...

Ngày tải lên: 08/03/2014, 16:20

... phosphatases in the regulation of yeast SAPK pathways, then fo cus on the properties of the protein phosphatases implicated in the mammalian SAPK systems. REGULATION OF SAPK SIGNAL PATHWAYS BY PROTEIN PHOSPHATASES ... pharmacological inhibition of protein phospha- tase 1 by 1 ,2-dioleoyl- sn-glycero-3-phosphate (PA); the activation of PP2A by high doses of PA, however, d...

Ngày tải lên: 17/03/2014, 23:20

... glycosylphophatidylinositol (GPI) anchors [1,2]. Although the primary structure of the glypi- cans is only marginally conserved (about 30% identity), there is a strict conservation of 14 cysteine ... amplified by relative quantitative RT-PCR as described previously [24]. Glyp-1, glypican-1; synd-1, syndecan-1; synd-4, syndecan-4. (A) Agarose gel of one representative experiment. (B) De...

Ngày tải lên: 17/03/2014, 23:20

... ADP-ribosylated by diphtheria toxin [25]. ADP-ribosylation inhibits the activity of eEF2. Phosphorylation of eEF2 inhibits its activity, in translo- cation and in poly(U)-directed polyphenylalanine synthesis [26,27], ... phosphorylation by MS6K may be involved in their regulation in response to serum in vivo. eEF1A and eEF1B are also substrates for phosphoryla- tion by the cla...

Ngày tải lên: 23/03/2014, 21:20

... Thr70, the sites most profoundly affected by rapamycin, may be regulated by nutrients through the mTOR pathway. REGULATION OF 4E-BP1 BY AMINO ACIDS In a number of kinds of mammalian cells, amino ... primarily by 1-leucine in isolated adipocytes. Am. J. Physiol. 44, C1232–C1238. 109. Lynch, C.J. (2001) Role of leucine in the regulation of mTOR by amino acids: revelation...

Ngày tải lên: 23/03/2014, 21:20