Báo cáo y học: "Identification of novel DNA methylation inhibitors via a two-component reporter gene system" pptx

... methyla- tion is considered as a therapeutically relevant strategy for cancer treatment. Among many agents with DNA methy- lation-modifying capability, 5-aza-2’-deoxycytidine (decita- bine; 5-Aza) ... as demonstrated by the US Food and Drug Administration approval of the DNA methyltransferase inhibitors azacytidine and 5-aza-2’-deoxycytidine for the treatment of myelodysplastic sy...

Ngày tải lên: 10/08/2014, 05:21

... Takeba Y, Shimoyama H, Nagafuchi H, Tak- eno M, Saito N, Yokoe T, Kaneko T, Asai T, Sakane T: Modulation by proinflammatory cytokines of Fas/Fas ligand-mediated apoptotic cell death of synovial ... R, Adachi M, Matsuzawa A, Kasugai T, Kitamura Y, Itoh N, Suda T, Nagata S: Lethal effect of the anti-Fas antibody in mice. Nature 1993, 364:806-809. 16. Okamoto K, Asahara H, Kobayashi T,...

Ngày tải lên: 09/08/2014, 07:20

... Suzuki A, Yamada R, Chang X, Tokuhiro S, Sawada T, Suzuki M, Nagasaki M, Nakayama-Hamada M, Kawaida R, Ono M, et al.: Functional haplotypes of PADI4, encoding citrullinating enzyme peptidylarginine ... decorin and biglycan. J Biol Chem 2001, 276:12201-12211. 29. Kizawa H, Kou I, Iida A, Sudo A, Miyamoto Y, Fukuda A, Mabuchi A, Kotani A, Kawakami A, Yamamoto S, et al.: An aspartic ac...

Ngày tải lên: 09/08/2014, 08:23

... use. RNA extraction and gene expression analysis RNA was extracted over RNeasy spin columns (Qiagen, Valencia, CA, USA) and tested for integrity and quantity on an Agilent 2100 Bioanalyzer (Agilent ... crystal inflammation. The marked rise of the upregulated mRNAs after the early surge in cytokine and Egr-1 mRNAs suggests that they may be part of a 'second wave' of factors...

Ngày tải lên: 09/08/2014, 10:23

... analysis of data. BLC performed the analysis of pooling data. HD and GS-D provided DNA samples, made a substantial contribution to the acquisition of data, and criti- cally revised the manuscript. AHS ... disease man- ifestations, management, and advances in treatment. Nat Clin Pract Rheumatol 2007, 3:148-155. 2. Mizuki N, Ota M, Katsuyama Y, Yabuki K, Ando H, Shiina T, Pal- imeris...

Ngày tải lên: 09/08/2014, 14:20

... liverF X X X X X X X X X XXX X XX X X X X X X X X X X XXX X X X X X X X X XXXX X X X X X X X X XX X X XX X X X X X X X X X X X X XXX X XXX X X XX X X X XXXX X X X X X X X X X X X X X X X X X X X X X X X X XX X X XX X XXX X X X X X X X X X X X X X X X X X X XX X X X X X X XXXX X X X X X X X X XX X X X XX X X X X X X X X X X X X X X X X X X X X XXX X X X X X X XX X X X XXX X X X X X X XXXX X XX...

Ngày tải lên: 09/08/2014, 20:22

... Table 2. Aggregate markers of cartilage longevity We evaluated combinations of biochemical and MRI-based markers for cartilage breakdown, quantity, and quality. Such combinations may exploit ... it may provide an aspect of quality), congruity, roughness, and homogeneity (markers for quality). We denote this aggregate marker longevity-tib. Finally, for comparison, we also evaluated an ag...

Ngày tải lên: 09/08/2014, 14:22

... the cDNA library, the BD serum was exten- sively adsorbed against bacteria and wild-type λZAP phage mixture to reduce background binding. Analysis of candidate cDNAs Purified candidate plaques ... pathophysiology of endothelial cells, and antibody to endothelial cell antigen (AECA) has been reported. Reports on the prevalence of AECA have varied largely and alpha-eno- lase was reported...

Ngày tải lên: 09/08/2014, 07:20

... patients investigated previous smoking history, coronary artery disease, diabetes mellitus and malignancy history in their family. Statistical Analysis Data files were analyzed initially ... cancer was di- agnosed on biopsy of a suspicious ulcerated area in a 45-year-old man patient. Partial gastrectomy was su ccessful and rema ins w el l. An 82 -year-old man was diagnosed adeno...

Ngày tải lên: 25/10/2012, 11:18

... designed as follows: 5’-TTAGGCCTAAAAATGGAGAACATCACATCAGGA-3’ 5’-AAAACAGAGACCCATATGTAAATTGGTACCAATT-3’ The amplified PCR product was cloned into the binding domain vector pTMBV4 in-frame with ... Construction of cDNA Library in S. cerevisiae DSY-2 Strain The cDNA library was prepared by transforming the yeast S. cerevisiae DSY-2 strain, in which the cDNA mixture was combined with pDL2...

Ngày tải lên: 02/11/2012, 11:08