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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt

Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt

Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt

... 17:64http://www.jbiomedsci.com/content/17/1/64Page 2 of 13RESEA R C H Open Access Overexpression of hTERT increases stem- like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines Chih-Chien ... article as: Tsai et al.: Overexpression of hTERT increases stem- like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines. Journal of Biomedical Science 2010 ... applica-tion of human mesenchymal stem cells (hMSCs), we have clonally isolated permanent and stable human MSC lines by ectopic overexpression of primary cell cultures of hMSCs with HPV 16 E6E7 and human...
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Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines ppt

Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines ppt

... tissues of cervicalcarcinomas corresponds to ou r finding of strong mRNAexpression in Caski and SiHa cell lines [13]. Fur ther, wefound a strong expression of MMP-15 mRNA in those cell lines. ... least one of the cell lines. For MMP-8, -12 and -21 only very weakmRNA expression could be observed in single cell lines. Nine MMPs, which were present in most of the cell lines, were chosen ... -fied in this cell line. In addition, expression of MMP-14 and -19 was detected but only on mRNA level.The highest expression found in all cell lines tested of the active protein forms of MMP-2 and...
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Báo cáo khoa học: Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation, expressed in human embryonic kidney 293T cells ppt

Báo cáo khoa học: Characterization of rat cathepsin E and mutants with changed active-site residues and lacking propeptides and N-glycosylation, expressed in human embryonic kidney 293T cells ppt

... human M cells [5], Langerhans cells, and interdigitating reti-culum cells [4]. However, fundamental information onthe biosynthesis, processing, and intracellular traffick-ing of this protein ... chains of ratcathepsin E in the transfected cells and then assessedthe role of N-glycosylation in its folding, processing,maturation, and subcellular trafficking. HEK-293Tcells expressing wild-type ... compartment.Interestingly, the intracellular localization of recom-binant cathepsin E appears to vary with the cell typeused. In mouse L cell and monkey Cos 1 cells, theexpressed human cathepsin E...
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báo cáo hóa học:

báo cáo hóa học:" Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets" pptx

... analysed using microarrays and quantitative PCR in several human CD8+T cell subsets defining the major steps of the T cell differentiation pathway.Results: We found expression of a limited set of ... transcriptional programs, and there-fore distinct range of receptors and intracellularproteins, indicating quite different requirements for sti-mulation and survival, homing po tential and effectorfunctions ... differentiation in humans remains largely unknown.Methods: In order to explore the potential implication of microRNAs in CD8+T cell differentiation in humans,microRNA expression profiles were...
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Báo cáo y học:

Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx

... being affected by specificchanges in methylation of the binding site, instead of more general methylation changes in the surroundingregion. In these comparisons we matched the two cell lines ... conservation and variability of DNA methylation across different stem cell lines. A recent analysis of about 1% of the genome of HESC lines shows that, by monitoring DNA methylation and gene expression, ... xpres-sion patterns in noncoding microRNAs between HESC lines result in distinct differentiation properties [49],indicating that epigenetic phenomena may be regulatingthese diverse differentiation...
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Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx

Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx

... PAGE in t hepresence of: (1) sa rcosine; (2) glycine; (3) N-ethylglycine; (4) N,N-dimethylglycine; (5)D-alanine; (6)L-alanine; (7)D-proline; (8)L-proline; (9)D-valine; (10)L-valine, ... Comparison of thethree-dimensional protein and nucleotide struc ture of the FAD-binding domain of p-hydroxybenzo ate hy droxy lase with the FAD-as well as NADPH-binding domains of glutathione ... partially overlaps withthat of DAAO and SOX and indicates an oxidative role of this oxidase in B. subtilis on a wide r ange of compounds.The c loning and overexpression of the B. subtilis GO alsoprovides...
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Báo cáo khoa học: Expression of CYP2E1 increases oxidative stress and induces apoptosis of cardiomyocytes in transgenic mice pdf

Báo cáo khoa học: Expression of CYP2E1 increases oxidative stress and induces apoptosis of cardiomyocytes in transgenic mice pdf

... College, Beijing, China2 Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Institute of Laboratory AnimalScience, Chinese Academy of Medical ... observations made in rat liver [21]. The expression of CYP2E1 is increased in the human ischemic and dilated heart [12] and in leftventricular tissue of the spontaneously hypertensive rats[13]. In the ... A rapid and sensitive method forthe quantitation of microgram quantities of proteinutilizing the principle of protein-dye binding. AnalBiochem 72, 248–254.CYP2E1 causes apoptosis of cardiomyocytes...
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The review of biomass pyrolysis oil properties and upgrading research

The review of biomass pyrolysis oil properties and upgrading research

... degree in Industrial Chemistry from the University of Limerick and is currently pursuing a PhD in the University. His supervisors are Prof Michael Hayes, Dr JJ Leahy and Dr Witold Kwapinski. ... Dr Witold Kwapinski. His PhD work involves studies of pyrolysis and second generation biorefining for the processing of carbohydrate waste materials and lignocellulose biomass to produce bio-oil. ... which he investigates is the residual bio-char which has value in soil amendment. He will also investigate the most efficient procedures (time, temperature and pressure) for the bio-refining process...
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Control of Membrane Fouling by Coagulant and Coagulant Aid Addition in Membrane Bioreactor Systems

Control of Membrane Fouling by Coagulant and Coagulant Aid Addition in Membrane Bioreactor Systems

... behavior of polysilicate in controlling membrane fouling in three MBR systems (R1, R2, and R3). TMP was increased by membrane clogging and recovered by physical washing. The concentrations of sole ... Journal of Water and Environment Technology, Vol. 8, No.3, 2010 Address correspondence to Jun Nakajima, Department of Environmental Systems Engineering, Faculty of Science and Engineering, ... polysilicate and Fe(III) was used in this study. Behaviors of these materials on biopolymer (protein and carbohydrate) removal and fouling reduction were investigated by conducting batch and long-term...
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How to Free Yourself of ANY Disease, Stop Fatigue, And Create Unstoppable Energy in Your Life.

How to Free Yourself of ANY Disease, Stop Fatigue, And Create Unstoppable Energy in Your Life.

... fascinating point: as acid increases in your body and keeps attacking your blood cells in this way, your blood cells literally EXPLODE. When your blood cells explode, they release acids of their ... taking in every day (Pop, Coffee, and Juice) and drink nothing but water, I saw the effects immediately. I had dandruff and all kinds of skin problems for years which disappeared in less than ... churning pressure in your stomach. That’s part of the acid which is building up in your blood as you indulge in these emotions. In fact most aches and pains are simply part of the process of...
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