Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx

... RESEA R C H Open Access Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK Hsiao-Yun Lin 1,2† , Chih-Chien ... phosphorylation status of Akt and ERK for stage III cells. There was a baseline of Akt and ERK phosphorylation without adding ECM. FN and...

Ngày tải lên: 10/08/2014, 05:21

... chondrogenic differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondro- progenitor cells [1,7-11]. During development of ... cultured human mesenchymal stem cells from marrow. Tissue Eng 1998, 4:415-428. 35. Mueller MB, Tuan RS: Functional characterization of hypertro- phy in chondro...

Ngày tải lên: 09/08/2014, 14:22

... loss of stem- like properties and spontaneous differentiation those hinder the expansion and applica- tion of human mesenchymal stem cells (hMSCs), we have clonally isolated permanent and stable human ... RT-PCR analysis of expression levels of hTERT and GAPDH, and (B) Real-time RT-PCR analysis of expression levels of DNMT1, DNMT3A and DNMT3B in primary h...

Ngày tải lên: 10/08/2014, 05:21

... the osteogenic differentiation of human mesenchymal stem cells (MSCs); and blockade of the activity of the BMP inhibitor increases the rate of osteogenic differentiation of human MSCs in vitro. Methods Human ... day 10 and by Ali- zarin Red stain at day 21. Figure 2 Expression of BMP-2 and chordin transcripts during osteogenic differ-entiation of human m...

Ngày tải lên: 09/08/2014, 10:23

... between EB and hES cells and between EB and adult cells were significant (P < 0.05). The differ- ence in expression of miR-17-5p between EB and hES cells, and of miR-20a between EB and adult cells ... hES cells than in either EB or adult cells (Figure 6, panel A). The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB...

Ngày tải lên: 18/06/2014, 15:20

... specific features of the plasticity of adult stem cells are largely unknown. Recently, we demonstrated the hepatic differentiation of human adipose tissue-derived mesenchymal stem cells (AT-MSCs). ... compilation ª 2008 FEBS A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells Yusu...

Ngày tải lên: 30/03/2014, 04:20

... generating activity of HL-60 Cells Utilizing the induced differentiation of HL-60 promyelo- cytic leukemia cells as a model of myeloid maturation, we examined the expression and location of Rac and PAK. The ... revealed that Rac and PAK isoforms increased in both cytosol and membrane fractions upon the induced differentiation of HL-60 cells. No remarkable Racwasseen...

Ngày tải lên: 31/03/2014, 15:20

... the context of self-renewal and regulation of lineage- specific differentiation of mesenchymal stem cells. In addition we review recent research on the concept of stem cell niche, and its relevance ... Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. J Cell Mol Med 2004, 8:301-316. 31. Tuan RS, Boland G,...

Ngày tải lên: 09/08/2014, 10:20

... triggers differentiation of HUMSCs into cardiomyocytes and maturation of HUMSCs derived cardiomyocytes. Admittedly, the detailed mechanism(s) of above effects of S1P on differentiation of and maturation ... Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing con...

Ngày tải lên: 10/08/2014, 05:21

... those for each of the MCAD mutants, K364R and R256T, and for the wild-type enzyme, in E. coli cells grown both at 31 °C and 37 °C, and the effect on activity (ferri- cenium assay), and tetramer ... affect mainly the initial folding and stability of the tetramer, and similarly lowered levels of tetramer in human cells would account for the observed elevation of indi...

Ngày tải lên: 20/02/2014, 02:21