Immobilizing topoisomerase I on a surface plasmon resonance biosensor chip to screen for inhibitors pptx

... DNA-TopI binders for interaction and for primary screening of TopI inhibitors. Conclusions: TopI immobilized on the chip retained its bioactivities of DNA binding and catalysis of intermediates ... Medicine, Taipei Medical University, Taipei, Taiwan, 3 Department of Internal Medicine, Taipei Medical University Hospital, Taipei, Taiwan, 4 Institute of Molecular Biology, Academia...

Ngày tải lên: 10/08/2014, 05:21

... thus possible that binding of at least some Gla domain- containing proteins to biological membranes will be sensitive to local variations in the Ca 2+ concentration in the immediate vicinity of ... Gla-domain has a conformation that is appropriate for membrane binding. Equilibrium binding isotherms The concentration dependence of factor X binding is shown in Fig. 2. It is apparent tha...

Ngày tải lên: 08/03/2014, 23:20

... R max , maximum analyte binding capacity; k on , association rate constant; k off : dissociation rate constant; K D , equilibrium dissociation constant. K D ¼ k off /k on ; v 2 statistical value in ... to PPARc, even at millimolar concentrations (Fig. 2C). SPR biosensor experiments require immobilization of a receptor or ligand on a surface and monitoring its binding to a se...

Ngày tải lên: 16/03/2014, 16:20

... electrophoresis (CE), surface plasmon resonance (SPR), and isothermal titration calorimetry (ITC). By CE, heparin-binding activity was readily apparent for both a regular peptide and a slightly N-terminally modified ... binding; surface plasmon resonance; capillary electrophoresis; isothermal titration calorimetry. Amyloid P component (AP) is a heparin-binding serum protein,...

Ngày tải lên: 31/03/2014, 23:20

... promising alternative for achieving high sensitivity, and selectivity at low concentrations. The optical properties of metallic nanomaterials arise from localized surface plasmon resonance (LSPR), which is ... 124:10596. 17. Hiep HM, Yoshikawa H, Saito M, Tamiya E: An interference localized surface plasmon resonance biosensor based on the photonic structure of Au nanoparticl...

Ngày tải lên: 21/06/2014, 04:20

... the spatial grid does not allow discontinuity of both permittivity and permeability to occur simultaneously. 3.6 Terminating The Simulation Domain A basic consideration with implementation of ... Figure 2.2 Schematic for the localization of photons by a metal-coated axicon prism [10] 33 Figure 2.3 Intensity distributions on a gold-coated axicon prism for radially polarized i...

Ngày tải lên: 11/04/2014, 13:49

... biochemical identification system. API Staph (BioMeri eux, USA) was used for identification of S aur- eus and API 20 A (BioMerieux, USA) for identification of P. aeruginosa, C. tetani and C. perfringens ... accurately identify the mixed aerobic-anaerobic infection, providing a reliable alternative to bacterial culture for rapid bacteria detection. Keywords: bacterial infection bi...

Ngày tải lên: 18/06/2014, 19:20

... contributions JW and YL have made substantial contributions to conception and design, data acquisition, analysis and data interpretation and are involved in draft ing and revising the manuscript. WF has made ... substantial contributions to conception and design . BZ, MC, TJ, PL, JH, KZ and WG have made substantial contributions to data acquisition, analysis and data interpretation. Mor...

Ngày tải lên: 20/06/2014, 03:20

... conversion of light into heat and lateral deforma- tion of the crystalline lattice of a semiconductor takes place due to inhomogeneous absorption of light, leading to formation of periodical structure ... formation of mechanically com- pressed layer at the irradiated surface due to increase of concentration of the interstitial atoms of Si at the surface in temperature gradient fie...

Ngày tải lên: 20/06/2014, 22:20

... NANO EXPRESS Open Access Plasmonic propagations distances for interferometric surface plasmon resonance biosensing Dominic Lepage, Dominic Carrier, Alvaro Jiménez, Jacques Beauvais and Jan ... nanofabrication team for their helpful participation. Authors’ contributions DL carried out the main conception and design of the SPR architectures, participated in the analysis and interpreta...

Ngày tải lên: 21/06/2014, 03:20