Báo cáo y học: "HLA-G DNA sequence variants and risk of perinatal HIV-1 transmission" pptx

... erythrocytes. The erythrocytes were classified into; control erythrocytes, erythrocytes incubated with either 2 mM of PS or 2 mM of PQ, and erythrocytes incubated with combination of PS plus ... 001). Table 1: Hemolysis profile of control erythrocytes and erythrocytes exposed to either PRV or PQ at concentra- tion 2 m mole /L NaCl g/L % of erythrocytes hemolys...

Ngày tải lên: 25/10/2012, 11:40

... 0.2 and 0.64 depending on the pH and the presence of NP-40; (d) Fig. 3. Inhibition of ATP and ADP hydrolysis activities of purified chicken liver ecto-ATPDase by azide. ATP and ADP hydrolysis ... 269) Ó FEBS 2002 Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase Aileen F. Knowles 1 , Agnes K. Nagy...

Ngày tải lên: 22/02/2014, 04:20

... a long form of Rab32 (see Discussion). Bacterial expression of Rab31 and Rab32 GSTRab31, GSTRab32 and the potentially GTPase- deđcient mutants of t hese proteins, GST Rab31 Q64L and GST±R ab32 Q85L , ... only Rab31 and not GST, GST Rab32 or GST±Rab5A (Fig. 7C). Similarly, antibody to Rab32 detected only Rab32 (and minor proteolytic fragments) (Fig. 7D). [ 35 S...

Ngày tải lên: 08/03/2014, 16:20

... RAMIG antibody at 37 °C, for 30 min. The cells were then fixed with formaldehyde, b locked with isotype control antibody and stained with the fluorescent antibody against the other protein, on ice. The ... for IL-2 stimulation was also investigated. This was the tyrosine phosphorylation of STAT3/STAT5, monitored through binding of anti-(phospho-tyr-STAT3/STAT5) Ig. As Fig. 5B sh...

Ngày tải lên: 17/03/2014, 23:20

... Position the needle tip at the very bottom of the solution in the vial stopper when drawing the solution into the syringe. Before removing the needle from the vial, pull the plunger all the way back ... in the literature [24], it is more likely that the reaction is due to the immunother- apy rather than the omalizumab. Finally, there is also the possibility tha...

Ngày tải lên: 08/08/2014, 21:20

... 4% formalin before embedding in paraffin, sectioning, and staining with haematoxylin and eosin. Sections obtained from the kidneys were also stained using the periodic acid-Schiff staining technique. Evaluation ... presence of extraglandular disease manifestations with the situation in the salivary glands, it would be of interest to investigate further the involvement of kid...

Ngày tải lên: 09/08/2014, 10:23

... as cloning and sequencing of the entire 16S rDNA to identify any bacterial DNA potentially present in the SF samples of patients with ReA, UA, and other forms of arthritis who were also ana- lyzed ... bacteria in the synovial samples, but were not long enough to identify the species level [12-14]. We previously used the broad-range PCR, cloning and...

Ngày tải lên: 09/08/2014, 14:22

... Party on Systemic Lupus Erythematosus: Morbid- ity and mortality in systemic lupus erythematosus during a 10-year period: a comparison of early and late manifestations in a cohort of 1,000 patients. ... and infection prophylaxis for high risk patients. Based on an estimated prevalence of 29% for serious infections among patients with SLE and the findings of R...

Ngày tải lên: 09/08/2014, 14:22

... polymorphic sites when designing DNA methylation assays. All annotation data can be manually revised and amended. Step 2: design of DNA methylation assays MethMarker implements automatic assay design ... values of the DNA methylation assays are calculated directly from the high-resolution DNA methylation profiles, using a set of method-specific rules. For COBRA, bi...

Ngày tải lên: 09/08/2014, 20:20

... small repeats. Assembly of the raw uncorrected reads was quite poor because of the very low quality suffixe s of many of the 124 bp reads. Thus, we compare assembly of quality trimmed reads (performed ... corrections by quality value and nucleotide to nucleotide type. During this initial pass, we only make simple, unambiguous corrections by abandoning low quality reads more aggres...

Ngày tải lên: 09/08/2014, 22:23

... the TFBM parameters. Similarly to the discov ery of breakpoint reuse in 2003 [3], there is currently only indirect evidence supporting the birth and death of fragile regions in chromosome evolution. ... intriguing implication of TFBM is that few regions in a genome are fragile at any given time raising a ques- tion of finding the currently active fragile regions...

Ngày tải lên: 09/08/2014, 22:23

... loaded in AGO2 require their 2′-O- methylation to remain stable in the presence of highly complementary targets [102]. Reciprocally, instability of Drosophila miRNAs loaded in AGO1 was induced by ... dsRNA, are methylated by HEN1 as single-stranded species [39-42]. In addition to this core machinery, several accessory factors in uence the biogenesis efficiency, fidelity and...

Ngày tải lên: 09/08/2014, 22:24

... pairs of HIV-1- infected mothers and their uninfected children and 20 pairs of mothers and children who were HIV-1- infected. Analysis of the HLA-G DNA sequences of exons 2, 3, 4, 6, 7, a part of ... restriction enzyme resulted in 85.7% (12/14) of the mother-child pairs showing dissimilarities of banding profiles among the HIV-1- non-transmitting group and 90% (18/...

Ngày tải lên: 10/08/2014, 05:20

... sequence alignment and drafted the manuscript. SP and KS participated in the sequence alignment. PL and YP participated in the design of the study and performed the data statistical analysis. YP conceived of the ... iden- tity with HRV-A and HRV-B, respectively. Direct investigation of the VP1 gene revealed that HR V-CU072 shared only 64% sequence identity...

Ngày tải lên: 11/08/2014, 21:21

... patients was waived by the local research/ethics commit- tee as this was an observational study looking at plasma DNA, a new variable measured from blood samples that were taken as part of standard ... for early risk stratification and prediction of inhospital and overall morbidity and mortality in a range of conditions including stroke [6], myocardial infarction [11]...

Ngày tải lên: 12/08/2014, 23:23