... Scientific,Nepean, ON, Canada), deparaffinized in xylene, rehydrated in a reverse-graded series of ethanol, and preincubated withchondroitinase ABC 0.25 units/ml (Sigma-Aldrich Canada,Oakville, ON, Canada) ... Lohmander LS, Neame PJ, Sandy JD: The structure of aggrecanfragments in human synovial fluid: Evidence that aggrecanasemediates cartilage degradation in inflammatory joint disease,joint injury, ... polyclonal goat antibody against collagenase-3 (MMP-13) (15 µg/ml; R&D Systems, Minneapolis, MN, USA); poly-clonal goat antibody against cathepsin K (1 µg/ml; Santa Cruz,Santa Cruz, CA, USA);...