Báo cáo y học: " Novel multi-component nanopharmaceuticals derived from poly(ethylene) glycol, retro-inverso-Tat nonapeptide and saquinavir demonstrate combined anti-HIV effect" potx

... following cytokines were measured: IL-1α, IL-1β, IL-5, IL-10, IL-12, IL-18, tumor necrosis factor-α (TNF-α), and IFN-γ. Cytokine analysis by lymphocyte intracellular staining and flow cytometry T-cell ... Carducci 15, 92100 Milan, Italy 6 Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands Correspo...

Ngày tải lên: 09/08/2014, 08:23

... together with binding to their cognate ligands. Importantly, ASV derived from VEGF receptor type 1 and Tie1, and to a lesser extent from VEGF receptor type 2 and fibroblast growth factor receptor ... by decreased joint inflammation and destruction. Conclusion The present study shows that unique ASV derived from receptors that play key roles in angiogenesis – namely, VEGF rec...

Ngày tải lên: 09/08/2014, 10:23

... contaminants and ensure a pure population of adipocytes. This method has been validated with flow cytometry to yield a 100% pure population of adipocytes. Subsequently, RNA was isolated from adipocytes and the ... activator inhibitor (PAI) 1[6]. Inflammatory cytokines produced in adipose tissue act locally and systemically to amplify the inflammatory cas- cade and oppose insulin sig...

Ngày tải lên: 11/08/2014, 03:20

... an assay sensitivity of < 7 pg/ml and an inter assay and intra assay variation of < 10%. Statistical analyses All data were checked for normality and then analyzed using the GLM model analysis. ... contaminants and ensure a pure population of adipocytes. This method has been validated with flow cytometry to yield a 100% pure population of adipocytes. Subsequently, RNA was isolat...

Ngày tải lên: 11/08/2014, 06:22

... S, Sinko PJ: Synthesis of poly(ethylene glycol)-based saquinavir prodrug conjugates and assessment of release and anti-HIV- 1 bioactivity using a novel protease inhibition assay. Bioconjug Chem ... manuscript.SP: Performed synthetic and quality control chemistry.OD: Performed HIV and general toxicity studies.MJL: Partici- pated in study and conjugate design and critically re...

Ngày tải lên: 10/08/2014, 05:20

... data on phyllokinin (braykinyl-isoleucyl-tyrosine-0-sulphate) and bradykinyl-isoleucyl-tyrosine. Br.J.Pharmacol.27, 479–485. 16. Yasuhara, T., Hira, M., Nakajima, T., Yanaihara, N. & Yanaihara, ... This sensitivity is well within the capability of the ES-MS system employed. The primary structural diversity displayed within amphibian skin bradykinins may not simply be due to phylogenetic...

Ngày tải lên: 21/02/2014, 15:20

... were purchased from Pharmacia. Bio-Gel HTP hydroxylapatite was from Bio-Rad. [a- 32 P]ATP, [ 14 C]phenylalanine and [ 3 H]GDP were purchased from Amersham. CTP, GDP, phosphoenolpyruvate and phosphoenolpyruvate ... PheRS was shown by the gel- shift assay and surface plasmon resonance technique. High stability of both novel ternary and quaternary complexes of eEF1AÆGDP, [eEF1AÆG...

Ngày tải lên: 21/02/2014, 15:20

... K. (2002) A novel rat hypothalamic RFamide-related peptide identified by immunoaffinity chromatography and mass spectrometry. FEBS Lett. 512, 255–258. 15. Fukusumi, S., Habata, Y. , Yoshida, H., ... Technology Corporation, Tokyo, Japan and 3 Suntory Institute for Bioorganic Research, Wakayamadai 1-1-1, Shimamoto-cho, Mishima-gun, Osaka, Japan Recently, we identified novel avian and amp...

Ngày tải lên: 08/03/2014, 16:20

... that these novel phosphatases play relevant roles in the biology of the yeast (cell integrity, cell c ycle regulation, cation homeostasis, etc) and that they interact with several key signalling pathways. ... residues directly following the conserved catalytic domain. This extension has no similarity with the C-terminal extension of type 2B catalytic subunits and ends with the highly con...

Ngày tải lên: 17/03/2014, 23:20

... were with glutamine at P1 and hydroxyl-bearing (Ser, Thr, Tyr) residues at P1¢. We do not know why PAP has amidolytic activity at neutral pH. But its speci®city differs from that of hK3/PSA, which also ... Miche Á le Ferrer 1 , Thierry Moreau 1 and Francis Gauthier 1 1 Laboratory of Enzymology and Protein Chemistry, INSERM EMI-U 00-10, University FrancË ois Rabelais, Faculty of Medici...

Ngày tải lên: 24/03/2014, 00:21