Báo cáo y học: "Systematic mapping of two component response regulators to gene targets in a model sulfate reducing bacterium" ppt
Báo cáo Y học: Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y pot
... hydrophilicity. The catalytic roles of
Cys341 in carboxypeptidase Y are examined by comparing
the kinetic parameters of the Cys341 mutant enzymes.
MATERIALS AND METHODS
Materials
Cbz-Phe-Leu-OH was obtained ... Site-directed mutagenesis of
virtually any plasmid by eliminating a unique site. Anal. Biochem.
200, 81–88.
15. Ito, H., Fukuda, Y. , Murata, K. & Kimura, A. (1983) T...
Báo cáo Y học: Subsite mapping of the binding region of a-amylases with a computer program ppt
... units;
pNP, 4-nitrophenyl group; PPA, porcine pancreatic a- amylase.
Enzymes: a- amylase (EC 3.2.1.1); porcine pancreatic a- amylase
(AMYP_PIG); barley a- amylase (AMY2_HORVU); rice a- amylase.
(Received ... found in the literature. Evaluations of subsite
maps of rice and barley a- amylases are thus also presented.
MATERIALS AND METHODS
SUMA
software: subsite mapping of amylas...
Báo cáo Y học: Co-existence of two regulatory NADP-glyceraldehyde 3-P dehydrogenase complexes in higher plant chloroplasts ppt
... kept in an artificial stroma
according to [20] containing various ions, sugars, and amino
acids (see Materials and methods) and in addition 140 l
M
NAD, 10 m
M
GSSG and BSA (20 mgÆmL
)1
)inorderto
simulate ... GAPDH-
containing aggregates: (a) the early evolved system, namely
PRK/GAPDH/CP12, which is activated merely by reduc-
tion and is already present in cyanobacteria [33]; and (b)...
Báo cáo y học: "Fine mapping of genes within the IDDM8 region in rheumatoid arthritis" pot
... identify variants that may contrib-
ute to susceptibility to RA and potentially to other autoimmune
diseases.
Materials and methods
Subjects
DNA was available for an initial RA dataset comprising ... for association with RA and
any significant associations were genotyped in a second
dataset of 174 RA cases and 192 controls, and the datasets
were combined before analysis. Associati...
Báo cáo y học: "Systematic analysis of genome-wide fitness data in yeast reveals novel gene function and drug action" pot
... lim-
ited amount of available high-quality data relating to drug
targets in yeast. We collected two high-quality training
sets: an expert-curated set of 83 yeast protein-compound
interactions, and yeast ... predic-
tions about any of these known interactions, presumably
due to the lack of sequence similarity to the available
training sets.
Thus, new sources of data and a...
Báo cáo y học: "The epidemiology of intensive care unit-acquired hyponatraemia and hypernatraemia in medical-surgical intensive care unit"
... University of Calgary, Calgary, AB T2N 2T9, Canada
3
Department of Medicine, University of Calgary, Calgary, AB T2N 2T9, Canada
4
Alberta Kidney Disease Network, Calgary, AB T2N 2T9, Canada
5
Calgary ... of ICU-acquired hyponatraemia and
hypernatraemia varies according to patient characteris-
tics.
• ICU-acquired hyponatraemia and hypernatraemia are
associated with increased risk...
Báo cáo y học: "The Geography of Chronic Obstructive Pulmonary Disease Across Time: California in 1993 and 1999"
... then areas that are farther away from
each other. When including areal information, such as
income by zip code or education by census tract in an
analysis, not taking into account area proximity ...
Using parametric modeling, we have demon-
strated in this analysis that geography matters, both
descriptively and analytically. The conventional ap-
proach of dealing with geography as...
Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf
... replacement of Asn147 was also
necessary to achieve a significant restriction in the cleavage
of the autolysis loop.
Autolytic inactivation of D-chymotrypsin and the
Phe114!Ile and Tyr146!His/Asn147!Ser
D-chymotrypsin ... substrate (or on succinyl-Ala-Ala-Pro-
Lys-NHMec for trypsin activity) at saturating concen-
trations (100 m
M). To investigate autolytic inactivation in
the...
Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx
... analysis using a
Hewlett Packard G202 5A LD-TOF system mass spectro-
meter and a- cyano-4-hydroxycinnamic acid as matrix.
Sample preparation
It has been shown that a trifluoroacetic acid pretreatment
renders ... strongly
hydrophobic side chains; this feature has been aptly
described by Rajan et al. as a Teflon coating that can
surround a helix [16] in the case of a mixture of...
Báo cáo Y học: Identification of novel membrane proteins by searching for patterns in hydropathy profiles potx
... the search strategy were
tested by applying it to a custom database containing
GABA
A
receptor sequences retrieved via a text search of
the Entrez database. GABA
A
receptors are also members of
the ... rise to a charac-
teristic pattern of peaks in their hydropathy profiles. To
conduct a search, each member of a polypeptide database is
converted to a hydropath...