Báo cáo y học: " Expanding whole exome resequencing into non-human primates" pot
Báo cáo y học: "Modeling human arthritic diseases in nonhuman primates" ppsx
... as the excretion rates of hydroxylysylpyridinoline (HP) (e) and lysylpyridinoline (LP) (f). The effect on body weight (b) was not statistically significant. Histology confirmed the lower-joint ... A healthy joint. The histology of a healthy rhesus monkey diarthrodial joint resembles that of humans, namely, a thin synovium lining the synovial cavity and a rather thick layer of hyaline cartilag...
Ngày tải lên: 09/08/2014, 06:23
Báo cáo Y học: Expanding the scorpion toxin a-KTX 15 family with AmmTX3 from Androctonus mauretanicus pptx
... previously described [9,15]. MALDI-TOF/MS was performed on a Perseptive DE-RP (Applied Biosystem) using a-cyano-4-hydroxycin- namic acid as matrix. Lethality assay in mice The in vivo toxicity of ... nmoles), S-alkylated with 4-vinyl-pyridine, were as previously described [11]. Treatment with pyroglu- tamate aminopeptidase unblocked the N-terminal glutamate residue [12–14]. An Applied Biosyst...
Ngày tải lên: 23/03/2014, 21:20
Báo cáo y học: "Thioglycosides as inhibitors of hSGLT1 and hSGLT2: Potential therapeutic agents for the control of hyperglycemia in diabetes"
... chemical synthesized [13, 14]. The most commonly used is known as T-1095 (3-(benzofuran-5-yl)-2',6'-dihydroxy-4'-methylpropio- phenone 2'-O-(6-O-methoxycarbonyl-β-D-glyco- pyranoside) ... Med. Sci. 2007, 4 137 tory effect of thioglycoside I (phenyl-1’-thio-β-D glucopyranoside) on hSGLT2 and thioglycoside VII (2-hydroxymethyl-phenyl-1’-thio-β-D-galactopyranosi de) o...
Ngày tải lên: 26/10/2012, 10:04
Báo cáo Y học: Rodent a-chymases are elastase-like proteases pot
... in human chymase [37,38]. They hydrolyzed the methylated substrate MeO-succinyl-Ala- Ala-Pro-Val-pNA more effectively than the unmethylated succinyl-Al-Ala-Pro-Val-pNA, predominantly due to the lower ... specificity, MCP-5s exhibited elastolytic activity. The amounts of DQÒ-elastin degraded by each enzyme were linearly related to the enzyme concentration (Fig. 4A), but their activity was relat...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo Y học: Role of critical charged residues in reduction potential modulation of ferredoxin-NADP+ reductase Differential stabilization of FAD redox forms doc
... stabilized by the wild-type enzyme ([SQ] ¼ 22.4% as determined by photoreduction experiments) are known. Thus, by simultaneously solving this Nernst derived two-equation system, the reduction potentials ... forms by tyrosine 94 and tryptophan 57 in flavodoxin from Anabaena and its influence on the reduction potentials. Biochemistry 36, 14334– 14344. 22. Pueyo, J.J., Go ´ mez-Moreno, C. & Ma...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo Y học: Molecular mechanisms underlying SHP-1 gene expression potx
... activity in SKOV3 cells. In our EMSA analyses, aside from the shifted band that contained USF1 and USF2, we observed other shifted bands (X and Y) . As bands X and Y were only partially inhibited by ... Research Institute, University Health Network, Toronto, Ontario, Canada; 2 Laboratory of Gynecologic Oncology, Department of Obstetric Gynecology and Reproductive Biology, Brigham and Women...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo Y học: Incorporation of 3-nitrotyrosine into the C-terminus of a-tubulin is reversible and not detrimental to dividing cells potx
... carboxypeptidase A is the physiological releasing enzyme in the post-translational tyrosination/detyrosination cycle. Activity of tubulin carboxypeptidase in both tyrosinated and nitrotyrosi- nated ... recognized by an antibody specific to nitrotyrosine (Fig. 1B) and was recovered without alteration after enzymatic or acid hydrolysis of [ 14 C]nitrotyrosinated tubulin. The mutual exclusion by...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Domain V of m-calpain shows the potential to form an oblique-orientated a-helix, which may modulate the enzyme’s activity via interactions with anionic lipid potx
... by the Royal Preston Hospital, UK. The phos- pholipids dimyristoylphosphatidylcholine (Myr 2 PtdCho), dimyristoylphosphatidylethanolamine (Myr 2 PtdEtn) and dimyristoylphosphatidylserine (Myr 2 PtdSer) ... dimyristoylphosphatidylcholine; Myr 2 PtdEtn, dimyristoylphosphatidylethanolamine; Myr 2 PtdSer, dimyristoylphosphatidylserine; FTIR, Fourier transform infrared; SUV, small unilamellar vesi...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Introducing Wilson disease mutations into the zinc-transporting P-type ATPase ofEscherichia coli The mutation P634L in theÔhingeÕ motif (GDGXNDXP) perturbs the formation of the E2P state pdf
... haracteristics. They retain 37% and 23% of the wild-type A TPase activity, respectively, and are poorly phosphorylated by ATP and P i . Low but significant ATPase activity and phosphorylation by ATP were ... catal ytic cycle of a P-type ATPase c an be characterized by determining the decay rate of t he phos- phorylated intermediate. The aspartyl-phosphate com- pound can decompose in two ways:...
Ngày tải lên: 17/03/2014, 17:20
Báo cáo Y học: Regulation of mammalian translation factors by nutrients pot
... kinases actually act on 4E-BP1 in vivo. Several of these, especially those acting at Ser65 and Thr70, the sites most profoundly affected by rapamycin, may be regulated by nutrients through the mTOR pathway. REGULATION ... Phosphory- lation of eEF2 at Thr56 inhibits its activity by preventing it from binding to the ribosome. Phosphorylation of eEF2 is catalysed by eEF2 kinase, an unusual and h...
Ngày tải lên: 23/03/2014, 21:20