Báo cáo y học: "Single cell transcriptomics of neighboring hyphae of Aspergillus nige" potx

... hybridization signal in each of the hyphae. A total of 207 different genes was found in this selection, of which 43 were found in all 5 hyphae and 119 were found exclusively in one of the hyphae. ... replicates of cDNA from pools of 100 tips or from cDNA from the whole periphery. The different yields of RNA obtained from the three sample types (that is, si n- gle hyphae, po...

Ngày tải lên: 09/08/2014, 23:20

... have been validated by the corresponding genomic DNA using a 250 K Affymetrix SNP array with the help of SNP copy number, loss -of- heterozygosity, and heterozy- gous SNPs. The karyotype of each EBV-transformed sample ... significantly improves the copy number variation detection in both simulated and real single -cell array CGH data. Background Array analysis of single -cell copy num...

Ngày tải lên: 09/08/2014, 23:20

... tissue 1 . They constitute approximately 0.6% of the laryngeal and hypopha- ryngeal benign tumors 4 . Laryngeal and hypopharyn- geal lipomas first appeared in literature in 1995 5 . Laryngeal lipomas ... dysphagia was the only symptom of large pyriform sinus lipoma. The preferred method of treatment for laryngeal and hypopharyngeal lipogenic tumors is radical endoscopic excision....

Ngày tải lên: 25/10/2012, 10:51

... Damjanovich 1,2 1 Department of Biophysics and Cell Biology, 2 Cell Biophysics Research Group of the Hungarian Academy of Sciences, University of Debrecen, Health Science Center, Debrecen, Hungary; 3 Institute of Molecular ... Simons, K. (1999) Clusters of glycolipid and glycosylphosphatidyl-inositol-anchored proteins in lymphoid cells: accumulation of actin regulated by loc...

Ngày tải lên: 17/03/2014, 23:20

... RL, Goronzy JJ, Weyand CM: T -cell- mediated lysis of endothelial cells in acute coronary syndromes. Circulation 2002, 105:570- 575. 84. Nakajima T, Goek O, Zhang X, Kopecky SL, Frye RL, Goronzy JJ, Weyand ... 41:369-375. 79. Weyand CM, Goronzy JJ, Liuzzo G, Kopecky SL, Holmes DR Jr, Frye RL: T -cell immunity in acute coronary syndromes. Mayo Clin Proc 2001, 76:1011-1020. 80. Liuzzo G, Kop...

Ngày tải lên: 09/08/2014, 01:23

... modification of proteins by ubiquitination. This system of protein ubiquitination plays a key role in many cellular processes, such as the regulation of the cell cycle, modulation of cell surface ... mutant mice may relate to an inability to functionally inactivate autoreactive lymphocytes, since Itch –/– T cells have been found resistant to the induction of anergy by low doses...

Ngày tải lên: 09/08/2014, 07:20

... B cell survival was demonstrated previously [44]. Figure 4 Immunocytochemical analysis of BAFF expression in salivary epithelilal cells from minor salivary glandsImmunocytochemical analysis of ... anti-human BAFF (Buffy-2) was kindly provided by Pascal Schneider (Apotech). Cell lines HSG is a cell line derived from neoplastic epithelial duct cells of the human salivary gland (a gi...

Ngày tải lên: 09/08/2014, 07:20

... monocyte purity and lym- phocyte purity were assessed by flow cytometry, and fractions were typically >80% and 90% pure, respectively. T -cell stimulation and fixation Elutriation-enriched lymphocytes ... 144:3347-3353. 13. Morita Y, Yamamura M, Kawashima M, Harada S, Tsuji K, Shibuya K, Maruyama K, Makino H: Flow cytometric single -cell analysis of cytokine production by CD4 + T cell...

Ngày tải lên: 09/08/2014, 08:23

... such an approach of apoptotic cell infusion has not yet been used directly in patients, the immunomodulatory properties of apoptotic cells may play a role in the tolerogenic effects of blood product ... analysis by flow cytometry (FACSCalibur ® ; BD Biosciences) using CellQuest Pro ® soft- ware (BD Biosciences). Cell culture and cytokine assays Peritoneal macrophages were obtained...

Ngày tải lên: 09/08/2014, 14:22

... Kim E: SALM synaptic cell adhesion-like molecules regulate the differentiation of excitatory synapses. Neuron 2006, 50:233-245. 16. Linhoff MW, Lauren J, Cassidy RM, Dobie FA, Takahashi H, Nygaard HB, ... identified members of this family by performing a comprehensive bioinformatics search of the zebrafish genome. Orthologues for each of the known type I membrane-tethered and glycop...

Ngày tải lên: 09/08/2014, 20:20