Báo cáo y học: " NF- B subunits RELB C-Rel RELA p50 p52 bound DNA b EMS" docx

... thereby the DNA is exposed to highly reactive ROS which induce single strand breaks in the DNA. In cells, generally, these toxic effects are eliminated by repair enzymes. Poly-(ADP-ribose)-polymerases ... ethidium bromide (EtBr) inter-calating into the DNA. For detection of the labeled DNA strands visu-alization by the Typhoon imaging methodology (GE Healthcare Europe GmbH, Germany) was used. ... Oxidative decay of DNA. J Biol Chem. 1997; 272: 19633-6. 55. Beckman KB, Ames BN. The free radical theory of aging matures. Physiol Rev. 1998; 78: 547-81. 56. Beckman KB, Ames BN. Mitochondrial...

... enzymes described in literature. (B1 ) enoyl-CoA hydratase/aldolase [26]; (B2 ) trans-o-hydroxybenzylidenepyruvate hydratase/al-dolase [29]; (B3 ) trans-2-carboxybenzalpyruvate hydratase/aldolase[32]; ... induction.Stability of citral lyase activityThe activity and stability of citral lyase was dramaticallyaffected by the addition of 20% (v/v) glycerol and 1 mMEDTA. When glycerol and EDTA were added beforedisrupting ... trans-o-hydroxy-benzylidenepyruvate hydratase/aldolase [29,30] (also knownas is 2¢-hydroxybenzalpyruvate hydratase/aldolase [31]),which is part of the naphthalene catabolic pathway (Figs 1and...

... HSP60were separated by on SDS/PAGE (6.5%polyacrylamide gel) followed by CoomassieBrilliant Blue staining (A), by immunoblottingwith an anti-(cytoplasmic HSP60) Ig (B) , orimmunoblotting with an ... wasrecognized by both the cytoplasmic and the mitochondrialHSP60, an antibody against the signal sequence of HSP60was recognized only by the cytoplasmic HSP60 in theimmunoblotting analysis. However, ... recently, it has been shownthat cytosolic (nonmitochondrial) HSP60 forms a macro-molecular complex with Bax and Bak14. The complexformation with HSP60 may block the ability of Bax andBak to...

... activity of t he peptide lacking the glycosylgroup was d emonstrated by injecting synthetic DUP9 9B (sDUP9 9B, s for synthetic; sDUP9 9B is not glycosylated)into the hemolymph of sexually mature, ... glycoproteins byN-glycosidase F [27], N-glycosidase A was used forde-glycosylation. The molecular mass of de-glycosylatedDUP9 9B was determined as 3748 Da by LC-MS, leaving1184 Da for the sugar moiety ... resolved by d e-glycosylationof mature DUP9 9B. As we suspected a1–3 fucosylation ofthe asparagine-linked G lcNAc, which had been shown toinhibit de-glycosylation of g lycopeptides or glycoproteins...

... mgÆmL)1BSA, 1 mMMgCl2(bufferA),andtoeachwellwasthenadded50lL inhibitor(in buffer A at double the final concentration) followed by50 lL biotinylated collagen I (also in buffer A at double t ... Gardner, H., Gotwals, P .J., Lobb,R.R. & Koteliansky, V.E. (2000) Regulation of inflamation bycollagen-binding integrins a 1b1 anda 2b1 inmodelsofhypersen-sitivity and a rthritis. J. Clin. Invest. ... NaCl/Tris,1mgÆmL)1BSA, then 50 lLcation(inNaCl/Tris,1mgÆmL)1BSA at double the final concentration) wasadded followed by 50 lL b iotinylated c ollagen I (also i nNaCl/Tris, 1 mgÆmL)1BSA at double the...

... substrate to the prostheticelectrophile at that time believed to be dehydroalanine.Because this mechanism cannot explain how the nonacidic b- H of the substrate can be abstracted by the enzymic ... MIO carbonyloxygen can be stabilized by partial protonation from N1-Hof the substrate. Analysis of the acidity/charge distributionin the r-complex intermediate in the PAL reaction bysemiempirical ... ofTechnology and Economics, HungaryThe plant enzyme phenylalanine ammonia-lyase (PAL,EC 4.3.1.5) shows homology to histidine ammonia-lyase(HAL) whose structure has been solved by X-ray crystal-lography....