Báo cáo y học: " NF- B subunits RELB C-Rel RELA p50 p52 bound DNA b EMS" docx

... and many would be RELARELA p5 0p50 p5 2p52 RELBp50 RELBp52 C-Relp52 RELAp52 RELAp50 C-Relp50 o n microarray common NF-< /b> B motif formed using 93 11-mer sequences 803 11-mer sequences o RELARELA dimer-specific ... 80 RELARELA RELAp50 RELAp52 p5 0p50 p50 p50 RELA RELA RELAp50 RELARELA RELAp50 RELAp52 p5 0p50 UV CTGGGGATTTA - UV - UV (nM) DNA- complex- EMSA DNA- complex- EMSA...

Ngày tải lên: 09/08/2014, 23:20

... was inhibited by the NF-< /b> B inhibitor dehydroxymethylepoxyquinomicin (DHMEQ). DHMEQ is a unique NF-< /b> B inhibitor designed in our laboratory based on the structure of the antibiotic epoxyquinomicin ... arrow (a), staining by anti -NF-< /b> B p65 anti- body; blue arrow (b) , staining by anti-NFATc1 antibody of the cells along the inner surfaces of bone lacunae. Open Access Availa...

Ngày tải lên: 09/08/2014, 10:21

... template, the B2 9/Ig -b promoter reporter B2 9-P was m ade by Pyrobest polymerase (TaKaRa, Kyoto, Japan) amplifica- tion of th e B2 9/Ig -b promoter (nucleotides )201 to +30) Fig. 2. DNase I hypersensitivity ... enhancer; B2 9-P, B2 9/Ig -b promoter. Luciferase activity indicated by arbitrary units. Ó FEBS 2002 Rat B2 9/Ig -b gene regulatory regions (Eur. J. Biochem. 269) 1231 expre...

Ngày tải lên: 24/03/2014, 03:21

... Berre R, Bor- dron A, Hutin P, Renaudineau Y,< /b> Dueymes M, Loisel S, Berthou C, Saraux A, Youinou P: BAFF-modulated repopulation of B lym- phocytes in the blood and salivary glands of rituximab-treated patients ... depletes B- cells in blood and lym- phatic tissue of cynomolgus monkeys. Transpl Immunol 2003, 12:19-28. 51. Vugmeyster Y,< /b> Beyer J, Howell K, Combs D, Fielder P, Yang...

Ngày tải lên: 09/08/2014, 14:22

... thereby the DNA is exposed to highly reactive ROS which induce single strand breaks in the DNA. In cells, generally, these toxic effects are eliminated by repair enzymes. Poly-(ADP-ribose)-polymerases ... ethidium bromide (EtBr) inter- calating into the DNA. For detection of the labeled DNA strands visu- alization by the Typhoon imaging methodology (GE Healthcare Europe GmbH, Ger...

Ngày tải lên: 25/10/2012, 11:15

... enzymes described in literature. (B1 ) enoyl-CoA hydratase/ aldolase [26]; (B2 ) trans-o-hydroxybenzylidenepyruvate hydratase/al- dolase [29]; (B3 ) trans-2-carboxybenzalpyruvate hydratase/aldolase [32]; ... induction. Stability of citral lyase activity The activity and stability of citral lyase was dramatically affected by the addition of 20% (v/v) glycerol and 1 m M EDTA. When glycerol and...

Ngày tải lên: 21/02/2014, 01:21

... HSP60 were separated by on SDS/PAGE (6.5% polyacrylamide gel) followed by Coomassie Brilliant Blue staining (A), by immunoblotting with an anti-(cytoplasmic HSP60) Ig (B) , or immunoblotting with an ... was recognized by both the cytoplasmic and the mitochondrial HSP60, an antibody against the signal sequence of HSP60 was recognized only by the cytoplasmic HSP60 in the immunoblotting analysis...

Ngày tải lên: 08/03/2014, 09:20

... activity of t he peptide lacking the glycosyl group was d emonstrated by injecting synthetic DUP9 9B (sDUP9 9B, s for synthetic; sDUP9 9B is not glycosylated) into the hemolymph of sexually mature, ... glycoproteins by N-glycosidase F [27], N-glycosidase A was used for de-glycosylation. The molecular mass of de-glycosylated DUP9 9B was determined as 3748 Da by LC-MS, leaving 1184 Da for th...

Ngày tải lên: 08/03/2014, 22:20

... mgÆmL )1 BSA, 1 m M MgCl 2 (bufferA),andtoeachwellwasthenadded50lL inhibitor (in buffer A at double the final concentration) followed by 50 lL biotinylated collagen I (also in buffer A at double t ... Gardner, H., Gotwals, P .J., Lobb, R.R. & Koteliansky, V.E. (2000) Regulation of inflamation by collagen-binding integrins a 1b1 anda 2b1 inmodelsofhypersen- sitivity and a rthritis. J. Clin....

Ngày tải lên: 08/03/2014, 22:20

... substrate to the prosthetic electrophile at that time believed to be dehydroalanine. Because this mechanism cannot explain how the nonacidic b- H of the substrate can be abstracted by the enzymic ... MIO carbonyl oxygen can be stabilized by partial protonation from N1-H of the substrate. Analysis of the acidity/charge distribution in the r-complex intermediate in the PAL reaction by semiempi...

Ngày tải lên: 08/03/2014, 23:20