Báo cáo y học: "A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language" ppt

... MS/MS Yes Yes Yes Yes Database of lipid masses Yes Yes Yes Yes Yes Yes Database of spectra Yes Database expandability Yes Yes Yes Yes Yes Isotopic correction Yes Yes Yes Yes Cross-platform Yes Yes ... 12:R8 http://genomebiology.com/2011/12/1/R8 Page 22 of 25 METH O D Open Access A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fr...

Ngày tải lên: 09/08/2014, 22:23

... reduces the risk for contamination, mini- mizes hands -on work and cuts down the reagent cost as only one amplification primer set is needed. Secondly, the sequencing is also simplified by reducing the ... sequence. Sequences interpreted by virco®TYPE The virco®TYPE interpretation based on a full length RT sequence (codon 1-400) was compared to the prediction based on...

Ngày tải lên: 10/08/2014, 05:21

... function as transcriptional or translational regulators, and that they may modify the function of a protein when the tandemly repeated region lies within the coding re- gion o f the gene [23]. Although ... identify susceptibility genes for EH [9]. The aim of the present study was to identify mu- tations or polymorphisms in the 5’-flanking region of the NPPB gene and to as...

Ngày tải lên: 26/10/2012, 10:04

... 2-amino- 5-carboxymuconic 6-semialdehyde is then converted to 2,5-pyridinedicarboxylic acid nonenzymatically (Fig. 4A). Therefore, we named the enzyme reported here 4-amino-3- hydroxybenzoate 2,3-dioxygenase. ... decreased the enzyme activity for 4-amino-3-hydroxybenzoic acid (0.16 m M )to85%. Inhibition by metal salts and other compounds Among the metal salts tested, the enzyme was...

Ngày tải lên: 21/02/2014, 01:21

... (4-hydroxy- 3-methoxyphenyl-b-hydroxypropionyl-CoA) into vanillin [28]. An other well known example is trans-o-hydroxy- benzylidenepyruvate hydratase/aldolase [29,30] (also known as is 2¢-hydroxybenzalpyruvate ... kJÆmol )1 for citral lyase activity was determined. The activation energy for the inactivation of the enzyme was determined to be 103.3 kJÆmol )1 . The pH dependence of...

Ngày tải lên: 21/02/2014, 01:21

... hydrogen bond in the E318I /Y/ W mutations, coupled with the hydrophobic character of the mutation, may facilitate conformational changes in the domain, for example by lowering t he energy barrier ... important in the gain-of-function effect. However, the side chain of E318 forms a hydrogen bond with R288 in the closed form, and this bond is brok en on moving to the open f...

Ngày tải lên: 08/03/2014, 22:20

... of the exact position of the two introns which were 780- and 630-bp long (Fig. 1B). These introns contained the typical GT/AG consensus splicing signal. The excision of the two introns from the ... actively replicating and virulent tachyzoites. The tachyzoites differentiate into encysted bradyzoites in response to the immune system attack during disease progression. They will...

Ngày tải lên: 08/03/2014, 22:20

... Stomatology, No.201 Military Hospital of PLA, Liaoyang, 111000, China * These authors contribute equally to the manuscript  Corresponding author: Gang Hu, Ph.D, M.D., Department of Dermatology, Second ... for Tissue Engineering, Fourth Military Medical University, Xi'an, 710032, China 3. Department of Oral Histology and Pathology, School of Stomatology, Fourth Military Medical Un...

Ngày tải lên: 08/08/2014, 18:20

... out contami- nating lymphocytes as a major source of IP-10 in the FLS–monocyte interactions. We examined the effect of mononuclear lymphocytes on IP-10 secretion in FLS lym- phocytes. SF monocytes ... primary antibody, and biotinylated polyclonal goat anti-IP-10 (0.1 µg/ml) served as the secondary antibody. The sensi- tivity limit for the IP-10 enzyme-linked immunosorbent assay...

Ngày tải lên: 09/08/2014, 01:21

... SF, and the proteolytic activity is monitored by the decrease of the fluorescence emitted by the microspheres via flow cytometry. For the analyses, a two-parameter histogram with the forward- angle ... protease inhibitors to the catalytic site of the activated forms of the enzymes. In the present work, we have measured by flow cytometry the net proteolytic activity in...

Ngày tải lên: 09/08/2014, 08:22