Báo cáo y học: "A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language" ppt
Báo cáo y học: "A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language" ppt
... MS/MS Yes Yes Yes YesDatabase of lipid masses Yes Yes Yes Yes Yes YesDatabase of spectra YesDatabase expandability Yes Yes Yes Yes YesIsotopic correction Yes Yes Yes YesCross-platform Yes Yes ... 12:R8http://genomebiology.com/2011/12/1/R8Page 22 of 25METH O D Open AccessA novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query languageRonny Herzog1,2†, ... Yes Yes Yes Yes Yes Yes YesSpectra alignment Yes YesGrouping Yes YesBatch mode Yes Yes YesOffset correction of masses YesaList of features: MS, lipid identification solely based on matching...
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Báo cáo y học: " A comparative analysis of HIV drug resistance interpretation based on short reverse transcriptase sequences versus full sequences" pps
... reduces the risk for contamination, mini-mizes hands -on work and cuts down the reagent cost asonly one amplification primer set is needed. Secondly, the sequencing is also simplified by reducing the ... sequence.Sequences interpreted by virco®TYPE The virco®TYPE interpretation based on a full length RTsequence (codon 1-400) was compared to the prediction based on the shortened RT sequence (codon 41-238).Figure ... approach on non-Bsubtypes (n = 17,131) was used for the Stanford HIVDRinterpretation algorithm.virco®TYPE HIVDR interpretation toolvirco®TYPE calculates the phenotypic drug susceptibilityfrom...
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Báo cáo y học: " A Novel Variable Number of Tandem Repeat of the Natriuretic Peptide Precursor B gene’s 5’-Flanking Region is Associated with Essential Hypertension among Japanese Females"
... function as transcriptional or translational regulators, and that they may modify the function of a protein when the tandemly repeated region lies within the coding re-gion of the gene [23]. Although ... identify susceptibility genes for EH [9]. The aim of the present study was to identify mu-tations or polymorphisms in the 5’-flanking region of the NPPB gene and to assess the relationship ... consistent with the hypothesis that Alu re-peat sequences in the 5’-flanking regions have regula-tory roles in NPPB expression [14]. Therefore, we as-sessed the association between mutations...
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Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc
... 2-amino-5-carboxymuconic 6-semialdehyde is then converted to2,5-pyridinedicarboxylic acid nonenzymatically (Fig. 4A).Therefore, we named the enzyme reported here 4-amino-3-hydroxybenzoate 2,3-dioxygenase. ... decreased the enzyme activity for 4-amino-3-hydroxybenzoic acid (0.16 mM)to85%.Inhibition by metal salts and other compoundsAmong the metal salts tested, the enzyme was completelyinhibited by 1 ... 4-amino-3-hydroxybenzoate cleavage. I,4-amino-3-hydroxybenzoic acid; II, 2-amino-5-carboxymuconic6-semialdehyde; III, 2-hydroxymuconic 6-semialdehyde; IV, 2,5-pyridinedicarboxylic acid. (B) Pathway...
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Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx
... (4-hydroxy-3-methoxyphenyl-b-hydroxypropionyl-CoA) into vanillin[28]. An other well known example is trans-o-hydroxy-benzylidenepyruvate hydratase/aldolase [29,30] (also knownas is 2¢-hydroxybenzalpyruvate ... kJÆmol)1 for citral lyase activity was determined. The activation energy for the inactivation of the enzyme was determined to be103.3 kJÆmol)1. The pH dependence of citral conversion by the purifiedenzyme ... is converted to form the aromacompound geranyl acetone. The citral lyase also convertedmethyl-crotonaldehyde and decanal forming acetone andoctanal, respectively. Furthermore, cinnemaldehyde...
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Báo cáo Y học: A novel gain-of-function mutation of the integrin a2 VWFA domain docx
... hydrogen bond in the E318I /Y/ W mutations, coupled with the hydrophobiccharacter of the mutation, may facilitate conformationalchanges in the domain, for example by lowering t he energybarrier ... important in the gain-of-function effect.However, the side chain of E318 forms a hydrogen bondwith R288 in the closed form, and this bond is brok en on moving to the open form. The loss of the hydrogen ... function of the aLandaM VWFA domains can be modulated by controlling the conformational state o f the domain. The majo rity ofapproaches have directly targeted the a7 helix, because of the large...
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Báo cáo Y học: A novel DNA repair enzyme containing RNA recognition, G-patch and specific splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii potx
... of the exact position of the two introns whichwere 780- and 630-bp long (Fig. 1B). These intronscontained the typical GT/AG consensus splicing signal. The excision of the two introns from the ... actively replicatingand virulent tachyzoites. The tachyzoites differentiate intoencysted bradyzoites in response to the immune systemattack during disease progression. They will remain in the brain ... that the proteinlevel is very low in the virulent tachyzoite. This conclusionwas supported by the control experiments where wemeasured the quality/quantity of total protein extracts byimmunoblots...
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Báo cáo y học: " A Novel Population of Mesenchymal Progenitors with Hematopoietic Potential Originated from CD14- Peripheral Blood Mononuclear Cell" potx
... Stomatology, No.201 Military Hospital of PLA, Liaoyang, 111000, China * These authors contribute equally to the manuscript Corresponding author: Gang Hu, Ph.D, M.D., Department of Dermatology, Second ... for Tissue Engineering, Fourth Military Medical University, Xi'an, 710032, China 3. Department of Oral Histology and Pathology, School of Stomatology, Fourth Military Medical University, ... Except for the value of mixed T lymphocytes and CD14+ cell-derived population, hematopoietic characters of cultured PBMMPs were indicated by CD14-/CD34+/CD45-/CD90+. The mes-enchymal...
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Báo cáo y học: "γ A novel mechanism for the regulation of IFN-γ inducible protein-10 expression in rheumatoid arthritis" ppsx
... out contami-nating lymphocytes as a major source of IP-10 in the FLS–monocyte interactions. We examined the effect ofmononuclear lymphocytes on IP-10 secretion in FLS lym-phocytes. SF monocytes ... primaryantibody, and biotinylated polyclonal goat anti-IP-10(0.1 µg/ml) served as the secondary antibody. The sensi-tivity limit for the IP-10 enzyme-linked immunosorbentassay was approximately ... reg-ulated not only by inflammatory cytokines but also by the physical interaction of activated leukocytes with FLSs.Once expressed, IP-10 probably plays a crucial role in the migration of Th1 cells...
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Báo cáo y học: "A novel approach to measure the contribution of matrix metalloproteinase in the overall net proteolytic activity present in synovial fluids of patients with arthritis" pptx
... SF,and the proteolytic activity is monitored by the decrease of the fluorescence emitted by the microspheres via flow cytometry. For the analyses, a two-parameter histogram with the forward-angle ... protease inhibitors to the catalytic site of the activated forms of the enzymes. In the present work, we have measured by flow cytometry the netproteolytic activity in synovial fluids (SF) collected ... SF, such as ELISA and zymography, do not providea direct measurement of their net proteolytic activity (NPA). The NPA depends on the activation status of the enzyme and on the balance between active...
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