Báo cáo y học: "Differential expression analysis for sequence count data" ppt

... read countsnottobeagoodestimatefortheratioof expected counts. Anders and Huber Genome Biology 2010, 11:R106 http://genomebiology.com/2010/11/10/R106 Page 2 of 12 MET H O D Open Access Differential expression analysis for sequence count data Simon ... MD, McCarthy DJ, Smyth GK: edgeR: a Bioconductor package for differential expression analysis of digital gene expression data....

Ngày tải lên: 09/08/2014, 22:23

... microscopy localization of the 11beta-hydroxysteroid dehydrogenase type I enzyme in the rat. Endocrinology 2001, 142:1644-1651. 20. Sun K, Myatt L: Enhancement of glucocorticoid-induced 11beta-hydroxysteroid ... 2005, 52:1711-1720. 40. Zhang TY, Ding X, Daynes RA: The expression of 11 beta- hydroxysteroid dehydrogenase type I by lymphocytes pro- vides a novel means for intracrine regulati...

Ngày tải lên: 09/08/2014, 08:22

... erythrocyte proteome: analysis by ion trap mass spectrometry. Mollecular und Cellular Proteomics 2004;3:501-509. 9. Tyan YC, Jong SB, Liao JD et al. Proteomic profiling of eryth- rocyte proteins by ... transcription and translation (for instance RNA polymerises I,II,III, zinc/PHD finger- DNA-binding proteins, cysteinyl, lysyl-tRNA syn- thetase), important RNA-stabilising factor - poly...

Ngày tải lên: 03/11/2012, 10:58

... unpublished data). Symbolic discriminant analysis of microarray data Relationships between gene expression variables are very complex and patterns that have clinical significance may take many forms. It ... for clinically distinct disorders within the autoimmune category. Consistent with this hypothesis, we have found that clustering analyses can be used to separate patients with early...

Ngày tải lên: 09/08/2014, 01:23

... Immunohisto- chemical analysis performed with anti-Tie1 from R&D Systems in synovial tissues supports its expression mainly on endothelial cells. However, western blot analysis for Tie1 also performed with ... used for microscopic analysis and statistical analysis are described in the supplementary material. Antibodies and immunohistochemistry Synovial tissues (4 µm) were fi...

Ngày tải lên: 09/08/2014, 03:24

... iodide (1 µg/ml; Sigma) was added immediately before cytometric analysis for the exclusion of dead cells. Flow cytometric analyses were performed by fluorescence-acti- vated cell sorting (FACS) ... immunity. Adv Immunol 2001, 78:57-110. 6. Moser B, Loetscher P: Lymphocyte traffic control by chemokines. Nat Immunol 2001, 2:123-128. 7. Buckley CD: Why do leucocytes accumulate within chronic...

Ngày tải lên: 09/08/2014, 06:23

... film (Eastman Kodak, Rochester, NY, USA). Cytofluorometry The expression of RANK-L and RANK at the membrane was evaluated by cytofluorometry. Freshly separated healthy human blood or SF neutrophils ... elimi- nated by hypotonic lysis. SF neutrophils were directly obtained by centrifugation over Ficoll-Paque. After two washes, cells were counted and resuspended in culture medium. Differentia...

Ngày tải lên: 09/08/2014, 10:20

... min. Cell lysates were examined by western blot analysis for pPyk2 or Pyk2 expression. The results are representative of three experiments. Inflam, inflammatory score; Vasc, vascularity score; ... pPLCγ on fibroblasts and lymphocytes. Discussion RA is a chronic inflammatory disease characterized by synovial hyperplasia. Proliferation of synovial cells leads to pannus for- mation resu...

Ngày tải lên: 09/08/2014, 10:21

... platforms [5]. When perform- ing systems biology analyses, failure to account for this effect will lead to biased results. One simple, but extremely widely used, systems biology technique for ... reproduction in any medium, provided the original work is properly cited. Method Gene ontology analysis for RNA-seq: accounting for selection bias Matthew D Young, Matthew J Wakefield, Gord...

Ngày tải lên: 09/08/2014, 20:21

... Northern blot analysis in both human lymphomas and human B-lymphocytes. The expression of the a-myb oncogene was shown to be higher (about 5 times) in lymphoma h1 (lane h1) than in lymphoma h2 ... analyzed by dot blot hybridization with [ 32 P]-labeled cDNA populations from SIV-associated monkey lymphomas and monkey B-lymphocytes. Those cDNAs whose hybridization signals were marked...

Ngày tải lên: 02/11/2012, 11:08