Báo cáo y học: "Capturing the dynamic epigenom" pot

... secondary to RA in the scientific and clinical perceptions of rheumatologists. The authors deal effectively with the challenges posed by the type and origin of their data. Although toxicity and ... and then at only those terminations for the reason of ineffectiveness and terminated for the reason of toxicity. This was done by censoring patients with treatment terminations other th...

Ngày tải lên: 09/08/2014, 14:20

... anticipated. These factors significantly reduced the power of the study. Based on their findings, the authors estimate that more than 100 hospitals would be needed to show the 30% difference they sought. ... seen from baseline to the study period for both groups combined. Conclusion The MET system greatly increases emergency team calling, but does not substantially affect...

Ngày tải lên: 25/10/2012, 10:39

... series will probably be familiar, it is hoped that there will still be useful lessons to be learned, for example the underlying assumptions of a hypothesis test that were not fully appreciated, or ... some previously unrecognised confusion between terms. The first article, presented in this issue, covers the presentation and summary of data. It is unlikely that the material covered by...

Ngày tải lên: 25/10/2012, 10:45

... the catalytic efficiency of the enzyme, the cold adaptation of the enzyme could not be attributed to the presence of the Gly cluster in this element. The present study supports the idea that the ... Gly261 by Ala in mutant G261A resulted in an enzyme with slightly lower stability. On the other hand, in the double mutant G261A /Y2 69A the additional replacement of Tyr269...

Ngày tải lên: 22/02/2014, 04:20

... guanidine hydro- chloride (GdnHCl), urea or elevated temperatures, they destroy the tertiary structure of proteins which results in the loss of enzymatic activity. Regarding the secondary struc- ture ... secondary structure only after the disruption of the native tertiary structure of RNase A (Figs 2–4). Interestingly, the preservation of the tertiary structure coincides with t...

Ngày tải lên: 22/02/2014, 07:20

... angle almost typical for the 3 10 helix. In this region of the polypeptide chain, the secondary structure is characterized also by the O i H N i þ 3 hydrogen bonds, found in all 40 DYANA structures ... residue for the final family is given in Fig. S1. Fig. 3A shows the family of 40 structures and Table 1 contains a summary of statistical data indica- ting the quality of the 40...

Ngày tải lên: 17/03/2014, 10:20

... catalytic activity proceed essentially by the same mechanism. Indeed, catalytic residues occupy the same position in the 3D structures of the two enzymes. In addition, the p redicted secondary structures ... function, display only one polar cavity in the vicinity of the conserved catalytic Asp re sidue. Keywords: neurospora; P-ATPase; homology model; cavity; H + . The 3D struc...

Ngày tải lên: 23/03/2014, 21:20

... previously described [9,15]. MALDI-TOF/MS was performed on a Perseptive DE-RP (Applied Biosystem) using a-cyano-4-hydroxycin- namic acid as matrix. Lethality assay in mice The in vivo toxicity of ... venoms and become key molecular probes for the characterization of these channels. They are usually small basic polypeptides (between 30 and 70 amino acids), cross-linked by three or four dis...

Ngày tải lên: 23/03/2014, 21:20

... Results from other coprecipitation studies supported the yeast two-hybrid results with the exception of the weak interaction observed between LEF-3 and IE1 with the yeast two-hybrid system (Table ... of PCR products into the BglII and PspAI sites of plasmid phsp70HAHisVI+ containing the 5¢ terminal amino acid sequence RYPYDVPDYARA. The sequence YPYDVPDYA constitutes the haemag...

Ngày tải lên: 23/03/2014, 21:20

... FEBS plasticity, between the F 1 and F o complexes, and the switching between tight and weak affinity may play a role in the coupling activity of the enzyme in some ATP synthases. Presently, there is ... binding to empty chip), allowing the elimination of the latter by baseline correction (see below). As a result of the location of the His tag on the very top of the c¢ sub-...

Ngày tải lên: 24/03/2014, 00:21