... cytotoxic. The reductions in cytotoxcitycompared to native ricin were comparable to the decrease in catalytic activity against ribosomes in vitro for each of the substitutions except for ricin ... ricin, d, ricin R213A, j;andricinR213D, m.(B)ricin,d; ricin R258A, j, and ricin R258D, m;(C)ricin,d; ricin R48A, j.Fig. 2. Assessment of the N-glycosidase activity of RTA variants con-taining ... 235 [9]. Of theses residues, only Arg213 is in thevicinity of the active site of RTA. In order to establish therole of arginyl residues around the active site of RTA in the binding of rRNA,...