báo cáo khoa học: "Modélisation dynamique de systèmes génétiques régulation L’induction de l’opéron lactose d’Escherichia coli étude qualitative et numérique du modèle" pot
Báo cáo khoa học: The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase pptx
... constants of PLP binding equilibrium and kinetic parameters determined with wild-type and mutant eSHMTs. Para- meters are expressed as the mean ± SD determined by nonlinear least squares fitting ... formation of the dimer induced by PLP binding is not accompanied by a complete recov- ery of the native tertiary structure. Discussion The decrease of hydrophobic contact area determined by the mut...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia coli transthyretin-related protein potx
... 98, 14943–14948. 31 Hammarstrom P, Jiang X, Hurshman AR, Powers ET & Kelly JW (2002) Sequence-dependent denaturation energetics: a major determinant in amyloid disease diversity. Proc Natl Acad Sci ... Umea ˚ University, Sweden 2 Department of Medical Biochemistry and Biophysics, Umea ˚ University, Sweden 3 Division of Cell Biology, Diabetes Research Centre, Linko ¨ ping University, Swe...
Ngày tải lên: 16/03/2014, 01:20
Báo cáo khoa học: Selection of full-length IgGs by tandem display on filamentous phage particles and Escherichia coli fluorescence-activated cell sorting screening doc
... direct binding method and a capture method were utilized to determine kinetic parameters. Briefly, PA63 was coupled to a CM5 chip to an equivalent of 750 response units (RU) by using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide ... and synthetic complementarity-determining-region diversity. Nat Biotechnol 23, 344–348. 17 Knappik A et al. (2000) Fully synthetic human combi- natorial antibod...
Ngày tải lên: 22/03/2014, 21:21
Báo cáo khoa học: Using directed evolution to improve the solubility of the C-terminal domain of Escherichia coli aminopeptidase P Implications for metal binding and protein stability pptx
... inability to bind metals. Metalloproteins can fold via metal-dependent or metal-independent pathways [20,21]. They may bind metal ions before polypeptide folding, after complete protein folding, ... domain AMPP#2 has a 157 amino acid deletion, AMPP#12 has a 212 amino acid deletion, AMPP#3-22 has a 157 amino acid deletion, and AMPP#4-3 has a 172 amino acid deletion. Mutations are R166G, G270V,...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: "Modélisation de la croissance des peuplements réguliers de hêtre : dynamique des hiérarchies sociales et facteurs de production" pptx
... MÉTHODES Les données sur lesquelles s’appuie cette étude proviennent du réseau français de placettes per- manentes de production pour le hêtre (Oswald, 1971 et 1981; Oswald et ... et multi- plicativement, les effets de l’âge, de la densité, de la dimension et du statut so- cial. La structure linéaire de l’équation faci- lite le traite...
Ngày tải lên: 08/08/2014, 23:22
Báo cáo khoa học: "Modélisation de la structure locale et simulation d’éclaircies dans un peuplement hétérogèn" docx
... l’effectif de la première classe est C1, les rangs des arbres de la première classe vont de 1 à C1; si l’effectif de la seconde classe est C2, les rangs des arbres de la seconde classe vont de C1+1 ... la construction du modèle de structure viendra ensuite. Elle généralise celle développée dans [5] en ce qu’elle intro- duit la notion de densité locale et permet de simule...
Ngày tải lên: 08/08/2014, 14:22
Báo cáo khoa học: "Modélisation mécanique des vibrations propres d’un arbre soumis aux vents, en fonction de sa morphologie M Fournier" ppt
... modélisation de la structure et une si- mulation numérique des balancements propres en vue d’effectuer des tests de sensibilité et de décider des données mor- phologiques et rhéologiques ... permet de distinguer 2 types de branches insérées sur le tronc, et nous suggère de ne retenir que les plus significatives du point de vue de...
Ngày tải lên: 08/08/2014, 23:22
Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
... the oxidation of thiol residues on the synthetic peptide and the oxidative refolding of reduced and denatured RNase A. In addition, neither protein reduced the disul de bond in insulin (Fig. 2C). ... family proteins have oxidative refolding activity on unfolded polypeptides and ⁄ or the ability to reduce disul de bonds [7,8]. To determine whether GmPDIL-3a or GmPDIL-3b possesses these activ...
Ngày tải lên: 18/02/2014, 11:20
Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus pdf
... marmoreus,andC. textile. Identical or similar residues are shaded in black or gray. The potential N-terminal signal peptides are boxed; the thioredoxin active sites are underlined; and the ER-retention motif ... Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China In eukaryotes, all proteins that travel along or reside in the secretory pathway are f...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulfide reductase from Pyrococcus horikoshii Implications for the disulfide metabolism of anaerobic hyperthermophiles doc
... The central metabolism of this organism uses an unusual NADPH-dependent sul de dehydrogenase which is capable of both the NADPH-dependent reduction of elemental sulfur and the NADP + -dependent oxida- tion ... 2.0 Oxidase-FAD d 22 5.9 a Determined at 200 lm CoA-S-S-CoA, b determined at 100 l m NADPH, c determined at 100 lm FAD, d determined at 100 lM NADH. CoADR from P. horikoshii D. R. Harr...
Ngày tải lên: 07/03/2014, 17:20