... repre-sents the mean of three independent measurementstogether with the mean error. These kinetic parameters in the absence of the osmolytes, shown in Table 1, are in excellent agreement with those ... DGD° values in the presence of these osmolytes are given in Table 1. How-ever, DGD° values of RNase-A in the presence of alanine, serine, lysine, b-alanine, taurine and dimethyl-glycine have ... decreased. On the otherhand, this change in conformation results in a decrease in kcat, the turnover number of the enzyme in the pres-ence of sugars, i.e. the maximum number of substratemolecules...