Báo cáo y học: " Strand-specific RNA sequencing reveals extensive regulated long antisense transcripts that are conserved across yeast specie" ppt

... article as: Yassour et al.: Strand-specific RNA sequencing reveals extensive regulated long antisense transcripts that are conserved across yeast species. Genome Biology 2010 11:R87. Submit your next ... 14 RESEA R C H Open Access Strand-specific RNA sequencing reveals extensive regulated long antisense transcripts that are conserved across...

Ngày tải lên: 09/08/2014, 20:22

... and finally to non-coding RNA classes (fRNAdb, database of ncRNA.org): piwi-interacting RNA (piRNA), tRNA, rRNA, small nucleolar RNA (snoRNA) and other non-coding RNA (ncRNA). Reads that did not ... also noted that 34.2% of reads that were mapped to the refer- ence genome did not correspond to any annotated small RNA. Our small RNA cloning strategy only captures small RNAs t...

Ngày tải lên: 09/08/2014, 23:20

... Pre-mRNA splicing Ro Y RNP Ro60 Cytoplasm RNA quality control La La RNP La protein Nucleoplasm Maturation RNA Pol III transcripts Th/To RNase MRP, RNase P Rpp38, Rpp25, Rpp20 Nucleoli Pre-rRNA ... were initially reported to be specifically associated with systemic lupus erythematosus [7], a later study showed that auto- antibodies to the Su antigen are found in a variety of system...

Ngày tải lên: 09/08/2014, 08:22

... report that showed a 60% decrease in the D/L Asp ratio in cartilage samples that were enzymatically depleted of proteoglycan [19] and with a more detailed study that showed that the majority of ... Matsushima Y, Kobayashi Y, Yamamoto Y: Age estima- tion by measuring the racemization of aspartic acid from total amino acid content of several types of bone and rib cartilage: a prelim...

Ngày tải lên: 09/08/2014, 13:22

... the Helsinki Dec- Accuracy of sequence variant calls compared with microarray genotype callsFigure 6 Accuracy of sequence variant calls compared with microarray genotype calls. The detection ... genotypes that match between sequence and microarray genotype divided by total comparisons. http://genomebiology.com/2009/10/10/R116 Genome Biology 2009, Volume 10, Issue 10, Article R116 Tewhey ......

Ngày tải lên: 09/08/2014, 20:20

... introduction of the dsRNA. This spread depends on the activity of an RNA- dependent RNA polymerase (RdRP), which amplifies dsRNA to produce more copies, which are then cleaved by the enzyme Dicer to ... argument that Elp1 provides an RNA- dependent RNA polymerase activity that generates the antisense strand. Unlike the gene for canonical RdRP, the elp1 gene is conserved i...

Ngày tải lên: 09/08/2014, 20:20

... Decay rates of expressed ncRNAs and asRNAs ncRNA/asRNA Decay rate [min] rnpB (RNase P sRNA) >20 ffs (SRP RNA) >20 ssrA (tmRNA) >20 ssrS (6S RNA; Yfr7) >20 Yfr4 >20 Yfr5 >20 Yfr2 ... >20 asRNA_04601 >20 Yfr16 >20 Yfr8 19.7 Yfr14 11.6 asRNA_17331 8.4 asRNA_17181 7.8 ncRNA_Yfr9 6.9 asRNA_15721 4.9 Yfr11 4.8 asRNA_04001 4.2 Yfr6 4.0 asRNA_38 3.5 asRNA_00641 3.4 asRNA_...

Ngày tải lên: 09/08/2014, 20:22

... or by oligo (dT). Most protocols also include a fragmentation step, typically RNA hydrolysis or nebulization, or alter- natively cDNA fragmentation by DNase I treatment or sonication. Many sequencing ... Donaghey 2 , John L Rinn 2,3 and Lior Pachter 1,4* Abstract The biochemistry of RNA- Seq library preparation results in cDNA fragments that are not uniformly distributed within the...

Ngày tải lên: 09/08/2014, 22:24

... spermidine and spermine, respectively. Both enzymes a re highly regulated and are subjected t o feedback control by c ellular polyamines. Control of cellular polyam- ines by rapid r egulated degradation ... how interaction with antizyme inhibitor [19,20,41–43] affects antizyme degradation. The observation that antizyme may be degraded inde- pendently of ODC raised the possibility t hat...

Ngày tải lên: 22/02/2014, 04:20

... Sauermann 1 1 Plant Physiology, University of Osnabrueck, Germany; 2 Planton GmbH, Kiel, Germany; 3 Plant Physiology, University of Kaiserslautern, Kaiserslautern, Germany Light/dark modulation ... their activity, brought about by a redox- modification at specific cysteine residues mediated by the ferredoxin/thioredoxin system [1]. The activity of each of these enzymes is adjusted by fine-tuning...

Ngày tải lên: 08/03/2014, 09:20