Báo cáo y học: " Cloud-scale RNA-sequencing differential expression analysis with Myrna" pps
Báo cáo y học: " Cloud-scale RNA-sequencing differential expression analysis with Myrna" pps
... Access Cloud-scale RNA-sequencing differential expression analysis with Myrna Ben Langmead, Kasper D Hansen, Jeffrey T Leek * Abstract As sequencing throughput approaches dozens of gigabases per day, ... [http://bowtie-bio.sf.net/myrna]. doi:10.1186/gb-2010-11-8-r83 Cite this article as: Langmead et al.: Cloud-scale RNA-sequencing differential expression analysis with...
Ngày tải lên: 09/08/2014, 20:22
Báo cáo y học: "Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer" ppsx
... of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer. Journal of Hematology & Oncology 2011 4:33. Figure 1 Patients with ... contributions YL and SY designed the study, interpreted data; YL wrote the manuscript; QX and BW reviewed diagnoses of pathology; YJ performed the experiment; HG performed the statistical...
Ngày tải lên: 10/08/2014, 21:23
Báo cáo Y học: Molecular cloning, bacterial expression and properties of Rab31 and Rab32 docx
... sequences may c onvey effector speci®city, whereas the Switch I and II domains primarily convey sensitivity t o the binding of GTP [8]. There is support f or this view from the crystal structure of ... coupled to keyhole limpet haemocyanin via their endogenous (Rab31) or added (Rab32, Rab11A) cysteine residues, using 4-(N-maleimidomethyl) cyclohexane-1-carboxylate. Rabbits were immunized by i...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Repression of FasL expression by retinoic acid involves a novel mechanism of inhibition of transactivation function of the nuclear factors of activated T-cells pptx
... activation of T lymphocytes. EMBO J. 9, 4425–4433. 30. Oda, Y. , Kinoshota, M. & Kakehi, K. (1997) Fluorometric assay of binding specificity of plant lectins to yeast cells by biotin-avidin system and ... cells with a c alcineurin i nhibitor such as CsA, whereby it i s translocated back to the cytoplasm [19,39]. While NFAT dephosphorylation is mediated by calcineurin, rephosphorylation...
Ngày tải lên: 24/03/2014, 03:21
Báo cáo Y học: Selection of effective antisense oligodeoxynucleotides with a green fluorescent protein-based assay Discovery of selective and potent inhibitors of glutathione S-transferase Mu expression doc
... on cell-free assays, e.g. RNAse H digestion screens and oligonucleotide scanning arrays [9]. As activity in cell-free assay may not always correlate with activity in cellular systems, we developed ... pro- tein (EGFP) are cotransfected with AS-ODNs to COS-7 cells. Subsequently, cells are analysed for expression of the EGFP fusion protein by flow cytometry. With the assay, we tested the...
Ngày tải lên: 31/03/2014, 15:20
Báo cáo y học: "Catabolic stress induces expression of hypoxia-inducible factor (HIF)-1α in articular chondrocytes: involvement of HIF-1α in the pathogenesis of osteoarthritis" ppt
... Production of hydrogen per- oxide by rabbit articular chondrocytes: enhancement by cytokines. J Immunol 1990, 145:690-696. 38. Tiku ML, Yan YP, Chen KY: Hydroxyl radical formation in chondrocytes and ... KK, Tokyo, Japan). Densitometry of the signal bands was analyzed with Image Gauge version 4.0 (FUJI Photo Film, Tokyo, Japan). Proteoglycan production in chondrocytes Chondrocyte activity w...
Ngày tải lên: 09/08/2014, 06:23
Báo cáo y học: "The in vivo expression of actin/salt-resistant hyperactive DNase I inhibits the development of anti-ssDNA and anti-histone autoantibodies in a murine model of systemic lupus erythematosus" ppt
... quantified as previously described [44] and expressed as arbitrary fluorescence units. The analysis was done blind and 50 glomeruli per section were analysed. Flow cytometry Flow cytometry was performed ... a comprehensive flow cytometry analysis was performed. Three- colour analysis of the splenic and thymic cell populations did not highlight any significant abnormalities (Table 1). Mo...
Ngày tải lên: 09/08/2014, 07:20
Báo cáo y học: "Metalloproteinase and inhibitor expression profiling of resorbing cartilage reveals pro-collagenase activation as a critical step for collagenolysis" pot
... at days 0, 1, 3, 5, 7, 8, 10, 12, and 14. Hydroxypro- line release was assayed as a measure of collagen degrada- tion, and glycosaminoglycan release was assayed as a measure of proteoglycan degradation ... 37°C, and one unit of inhibitory activity inhibits two units of collagenase by 50%. Gelatinase activity in the culture supernatants was assayed by gelatin zymography. Samples Open Access Av...
Ngày tải lên: 09/08/2014, 08:22
Báo cáo y học: "The renal metallothionein expression profile is altered in human lupus nephriti" doc
... inflammatory and proteinuric conditions associated with lupus nephritis also display a significantly altered expression pattern of MT-I+II. By immunohistochemical analyses, we noted a relatively high ... Pathology, Herlev University Hospital, Professor Helle Dybdahl, Department of Pathology, Aarhus University Hospital, and Professor Niels Marcussen, Department of Pathology, Odense Univ...
Ngày tải lên: 09/08/2014, 10:23
Báo cáo y học: "Upregulated miR-146a expression in peripheral blood mononuclear cells from rheumatoid arthritis patients" doc
... prednisone, mycophenolate mofetil SjS1 Female 30 Hydroxychloroquine, mycophenolate mofetil SjS2 Male 21 Hydroxychloroquine SSc1 Female 57 Cyclosporine, hydroxychloroquine, prednisone, mycophenolate ... individuals, including one with systemic lupus erythematosus, two with Sjögren's syndrome, and one with systemic sclerosis, were included. Nine healthy donors with no history of a...
Ngày tải lên: 09/08/2014, 13:21