Báo cáo y học: "Global fitness profiling of fission yeast deletion strains by barcode sequencing" pptx

... accurate barcode sequences in a hap- loid fission yeast deletion library and validated them by conducting fitness analysis of barcoded fission yeast dele- tion strains in pooled cultures. The barcode ... characterization of the barcode sequences in the deletion library and describe a fitness- profiling pipeline that allows the analysis of a fission yeast h...

Ngày tải lên: 09/08/2014, 20:22

... S, Takahara M, Miyagishima SY, Mori T, Nishida K, Yagisawa F, Yoshida Y, Nishimura Y, Nakao S, Kobayashi T, Momoyama Y, Higashiyama T, Minoda A, Sano M, Nomoto H, Oishi K, Hayashi H, Ohta F, ... cerevisiae. Library richness and diversity The richness and diversity of cDNAs sampled from each cDNA library was estimated by statistical methods. Richness was estimated by rarefaction using...

Ngày tải lên: 09/08/2014, 20:22

... Bio- Plex syst em, which makes use of Luminex multi-analyt e profiling technology allows for the analysis of many different cytokines in a single microtiter well. Using this technology, we were ... complex with cytokine expression strongly influencing HIV infection and replication. Methods: We determined the expression patterns of 10 cytokine s by Luminex multi-analyte profiling i...

Ngày tải lên: 10/08/2014, 05:21

... mastocytoma P-815 was readily phosphorylated by CaM kinase II. Although the TPH from P-815 cells was easily phosphorylated by this kinase, its enzymic activity was not altered by the phosphorylation ... 5-hydro- xytryptophan and 5-hydroxytryptamine from tryptophan by neoplastic mouse mast cells. Biochim. Biophys. Acta. 111, 67–78. 3. Grahame, S.D. (1967) The biosynthesis of 5-hydrox...

Ngày tải lên: 08/03/2014, 16:20

... signifi- cance, range of assembly software, and (most critically) the availability of low level assembly data including the place- ments of reads in contigs. We chose to analyze the UMD2 cow assembly over the ... correction of false segmental duplications caused by genome mis-assembly Genome Biology 2010, 11:R28 Kelley and Salzberg Genome Biology 2010, 11:R28 http://genomebiology....

Ngày tải lên: 09/08/2014, 20:21

... for the fatty acid biosynthesis pathway only three enzymes were detected: 3-oxoacyl-[acyl-carrier-protein] synthase II (FabF), acetyl-CoA carboxylase (EC 6.4.1.2, 6.3.4.14) and [acyl-carrier-protein] ... the same three enzymes of the fatty acid biosynthesis pathway, it seems impossible that this pathway was lost by chance during sequencing and assembling of the three genomes by di...

Ngày tải lên: 09/08/2014, 23:20

... (R). Primary PCR was done with 10 cycles of 95°C for 5 sec and 70°C (-0.5°C per cycle) for 2 min fol- lowed by 20 cycles of 95°C for 5 sec and 65°C for 2 min. Nested PCR was done by using 1/250 ... infection by restricting viral entry. These studies have demonstrated for the first time the utility of the non-viral SB system to derive viral resistant cells and paved the way for the...

Ngày tải lên: 10/08/2014, 05:21

... phenotype of a short tau and eventual circadian arrhythmicity in DD is lost and a wildtype phenotype is rescued. The level of perturbation of the clock by Period deletion thus seems highly depend- ant ... beginning of the dark might result from the fact that activity is induced by the light exposure and not by the culturing technique. In our case, release of SCN-AVP induced...

Ngày tải lên: 10/08/2014, 09:20

... progressive symptoms of uterine bleeding which do not respond to conservative therapy [1-4]. Most of these lesions are asymptomatic and are found incidentally, but sometimes they may cause abnormal ... diagnosis of patients with vaginal bleeding. Hysterectomy is the primary mode of treatment in most symptomatic cases. Introduction Cavernous hemangiomas of the uterus are extremely r...

Ngày tải lên: 11/08/2014, 12:20

... activity against E. coli by the liquid-growth inhibition assay as described previously [8]. Peptide structure The purity of the peptides was confirmed by capillary zone electrophoresis and MALDI-TOF ... [14]. RESULTS Purification and primary structure of bass hepcidin Two fractions with antimicrobial activity from the gill of hybrid striped bass were purified to homogeneity by two ad...

Ngày tải lên: 24/03/2014, 03:21