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Báo cáo y học: "Phylogenetic detection of numerous gene duplications shared by animals, fungi and plants" ppsx

Báo cáo y học:

Báo cáo y học: "Phylogenetic detection of numerous gene duplications shared by animals, fungi and plants" ppsx

... d).Discussion Detection of very ancient eukaryotic gene duplications In this study, we investigated the extent of eukaryotic gene duplication before the divergence of plants and animals /fungi by constructing ... y more gene duplicatio nFigure 1 Alternative views of the eukaryotic phylogeny and the design of phylogentic analysis. (a) The ‘crown-stem’ topology of eukaryotic phylogeny. The topology shown ... analysis and dataexploration. Bioinformatics 2008, 24:1650-1651.doi:10.1186/gb-2010-11-4-r38Cite this article as: Zhou et al.: Phylogenetic detection of numerous gene duplications shared by animals,...
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Báo cáo y học: "Phylogenetic assessment of alignments reveals neglected tree signal in gaps" docx

... size of 60sequences by removing sequences randomly from sets of excessive cardinality.Definition: absolute minimum number of duplications For any set of homologous genes, consider partitions of the ... What is the impact of align-ment uncertainty on tree inference?Phylogeny-based tests of alignment accuracyThe principle of the phylogeny-based tests of alignmentaccuracy is simple: the more ... superior standards for assessing alignment accuracy, and paves the way for the development of phylogenetic inference methods of significantly higher resolution.BackgroundThe study of biological...
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Báo cáo y học: "Systematic detection of putative tumor suppressor genes through the combined use of exome and transcriptome sequencin" ppt

... suppressor genes shared by many cancer types likeRB1 and TP53, many tumor suppressor genes are specificto a particular tumor type and/ o r cell type that originatethe tumor. Deficiency of BRCA1 and ... comprise5.4% and 2.6% of genes surveyed, respectively. Many of these genes identified by loss of heterozygosity and allele-specific expression are kno wn or putative tumor suppressor genes, such ... as candidate cancer genes (CAN gene) by integrated a nalysis of homozygous deletions and sequence alterations in breast and colorectal cancers[34]. Meanwhile, epigenetic silencing caused by methyla-tion...
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Báo cáo y học: "Effective detection of rare variants in pooled DNA samples using Cross-pool tailcurve analysis" ppsx

... For all samples validated by Sanger sequencing, homozygous wildtypes are indicated by “-“, heterozygotes are indicated by a “+”, and homozygous mutants are indicated by a “++”. Additional ... study, and Dr. Manuel Rivas of the Massachusetts Institute of Technology and the University of Oxford with assistance in running Syzygy. This work was supported in part by a pilot grant (#2487) ... already fairly generous, chiefly eliminating variant-pools with clearly erroneous tailcurve ratios. This default was used for all our datasets. The combination of filtering by average quality and...
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Báo cáo y học: "Early Detection of cytokine protein expression in mouse lung homogenates using suspension bead array" ppt

... inflammatory cascade defined by early production of TNFα followed by subsequent production of IL-1, IL-6 [8] and MIP-2 [2]. The production of TNFα by macrophages and the recruitment of inflammatory cells ... pg/ml of IL-2) detected using antibody array. In thisstudy, antibody arrays findings suggested significantlyincreased expression of IL-6 and -13 and L-selectin pro-teins and significantly decreased ... purposes)The alignment of the 22 analytes on the RayBio Mouse Cytokine Antibody ArrayFigure 2The alignment of the 22 analytes on the RayBio Mouse Cytokine Antibody Array. Abbreviations: bFGF:...
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Báo cáo y học: " Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR" doc

... copies and one copyper sample were also run to est imate the limit of detec-tion (LOD) of the assay.Reproducibility and specificity of the assayThe standard PCV2 plasmid with 107,105 and ... vecommons.org/licenses /by/ 2.0), which permits unrestricted use , distribution, and reproduction inany me dium, provided the original work is properly cited. detection of PCV2 samples by 18% over that achieved by conventional ... pig mortality from 2-3% to 14-30% [13]. Ther e-fore, rapid and sensitive detection and quantitationassays for PCV2 are urgently needed both by the pigindustry and research commun ity. In comparison...
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Báo cáo y học: " Early detection of Varicella-Zoster Virus (VZV)-specific T-cells before seroconversion in primary varicella infection: case report" potx

... reacti-vation from latency. Varicella is typically diagnosed by characteris tic clinical signs and usually does not requirelaboratory testing. Due to the introduction of mass vac-cination programmes, ... diagnosed by virus isolation from vesicular fluid (v.f.) and by quantitative PCR for VZV-DNA out of v.f. or plasma (pl.) with Ct 16 and Ct 36,respectively. A third blood sample taken at day 6 p.r.o. ... the laboratory diagnosis of early varicella,because they are detectable in a time-delayed manner[5]. Therefore, the method of choice for the rapid diag-nosis of varicella is polymerase chain...
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Báo cáo y học: " Phylogenetic analyses of the polyprotein coding sequences of serotype O foot-and-mouth disease viruses in East Africa: evidence for interserotypic recombination" pps

... al. Virology Journal 2010, 7:199http://www.virologyj.com/content/7/1/199Page 7 of 9RESEARC H Open AccessPhylogenetic analyses of the polyprotein codingsequences of serotype O foot -and- mouth ... region of serotype OFMD viruses from Kenya and Uganda has been undertaken to infer evolutionary relationships and processesresponsible for the generation and maintenance of diversity within ... Belsham3AbstractBackground: Foot -and- mouth disease (FMD) is endemic in East Africa with the majority of the reported outbreaksattributed to serotype O virus. In this study, phylogenetic analyses of the polyprotein...
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Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx

Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx

... environment indiverse ways [2]. In general the macrostructure of phyco-bilisomes consists of a core (constituted by allophycocyanin)surrounded by phycocyanin and phycoerythrin (whenpresent) ... chromatogram abundant in hydrophilic proteinssuch as linkers and a significant amount of a -and b-phycocyan ins, and only traces of the allophycocyanins.On the contrary, HPLC a nalysis of bands 2–5 revealed ... phycocyanin and allophycocyanin are composed of two polypeptide chains,a and b, of approximately 17 000 and 18 000 [7]. The a and b polypeptides contain one or two chromophores, respect-ively....
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Báo cáo Y học: Bromoperoxidase activity of vanadate-substituted acid phosphatases from Shigella flexneri and Salmonella enterica ser. typhimurium doc

Báo cáo Y học: Bromoperoxidase activity of vanadate-substituted acid phosphatases from Shigella flexneri and Salmonella enterica ser. typhimurium doc

... Molecular Chemistry, University of Amsterdam, The Netherlands;2Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School and College of Biological Sciences, ... assay kit (Bio-Rad) with BSA as the standard.Enzymatic assay of phosphatase activityThe phosphatase activity was measured by hydrolysis of 10 mMp-nitrophenyl phosphate as a substrate in 100 ... form of these enzymes which is re-activated by vanadate. Thisre-activation is competitively inhibited by structural ana-logues of vanadate (tetrahedral compounds) such as phos-phate and molybdate...
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