Báo cáo y học: "Phylogenetic detection of numerous gene duplications shared by animals, fungi and plants" ppsx

... d). Discussion Detection of very ancient eukaryotic gene duplications In this study, we investigated the extent of eukaryotic gene duplication before the divergence of plants and animals /fungi by constructing ... y more gene duplicatio n Figure 1 Alternative views of the eukaryotic phylogeny and the design of phylogentic analysis. (a) The ‘crown-stem’ topology o...

Ngày tải lên: 09/08/2014, 20:21

... size of 60 sequences by removing sequences randomly from sets of excessive cardinality. Definition: absolute minimum number of duplications For any set of homologous genes, consider partitions of the ... What is the impact of align- ment uncertainty on tree inference? Phylogeny-based tests of alignment accuracy The principle of the phylogeny-based tests of alignment accu...

Ngày tải lên: 09/08/2014, 20:21

... suppressor genes shared by many cancer types like RB1 and TP53, many tumor suppressor genes are specific to a particular tumor type and/ o r cell type that originate the tumor. Deficiency of BRCA1 and ... comprise 5.4% and 2.6% of genes surveyed, respectively. Many of these genes identified by loss of heterozygosity and allele- specific expression are kno wn or putative...

Ngày tải lên: 09/08/2014, 22:23

... For all samples validated by Sanger sequencing, homozygous wildtypes are indicated by “-“, heterozygotes are indicated by a “+”, and homozygous mutants are indicated by a “++”. Additional ... study, and Dr. Manuel Rivas of the Massachusetts Institute of Technology and the University of Oxford with assistance in running Syzygy. This work was supported in part by a...

Ngày tải lên: 09/08/2014, 23:20

... inflammatory cascade defined by early production of TNFα followed by subsequent production of IL-1, IL-6 [8] and MIP-2 [2]. The production of TNFα by macrophages and the recruitment of inflammatory cells ... pg/ml of IL-2) detected using antibody array. In this study, antibody arrays findings suggested significantly increased expression of IL-6 and -13 and L-selectin...

Ngày tải lên: 11/08/2014, 08:21

... copies and one copy per sample were also run to est imate the limit of detec- tion (LOD) of the assay. Reproducibility and specificity of the assay The standard PCV2 plasmid with 10 7 ,10 5 and ... vecommons.org/licenses /by/ 2.0), which permits unrestricted use , distribution, and reproduction in any me dium, provided the original work is properly cited. detection of PCV2 s...

Ngày tải lên: 11/08/2014, 21:21

... reacti- vation from latency. Varicella is typically diagnosed by characteris tic clinical signs and usually does not require laboratory testing. Due to the introduction of mass vac- cination programmes, ... diagnosed by virus isolation from vesicular fluid (v.f.) and by quantitative PCR for VZV-DNA out of v.f. or plasma (pl.) with Ct 16 and Ct 36, respectively. A third blood s...

Ngày tải lên: 12/08/2014, 04:20

... al. Virology Journal 2010, 7:199 http://www.virologyj.com/content/7/1/199 Page 7 of 9 RESEARC H Open Access Phylogenetic analyses of the polyprotein coding sequences of serotype O foot -and- mouth ... region of serotype O FMD viruses from Kenya and Uganda has been undertaken to infer evolutionary relationships and processes responsible for the generation and maintenance of div...

Ngày tải lên: 12/08/2014, 04:20

... environment in diverse ways [2]. In general the macrostructure of phyco- bilisomes consists of a core (constituted by allophycocyanin) surrounded by phycocyanin and phycoerythrin (when present) ... chromatogram abundant in hydrophilic proteins such as linkers and a significant amount of a -and b-phycocyan ins, and only traces of the allophycocyanins. On the contrary, HPLC a...

Ngày tải lên: 08/03/2014, 16:20

... Molecular Chemistry, University of Amsterdam, The Netherlands; 2 Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School and College of Biological Sciences, ... assay kit (Bio-Rad) with BSA as the standard. Enzymatic assay of phosphatase activity The phosphatase activity was measured by hydrolysis of 10 m M p-nitrophenyl phosphate...

Ngày tải lên: 24/03/2014, 03:21