Báo cáo y học: " The yin and yang of chromatin spatial organization" ppt
Báo cáo y học: " The yin and yang of chromatin spatial organization" ppt
... of chromatin may extend away from the core of the territory. (d) As G1 phase is established and nucleoli form, loops of NOR-containing chromatin co-associate with the other components of the ... nuclear lamina. These processes, along with others described in this article and many more, are likely be important in dynamically shaping the spatial environment and organization of the genome.Specialized ... non-random. Preferential chromatin interactions, both in cis and in trans and between transcriptionally active and silent regions, inuence organization.© 2010 BioMed Central Ltd The yin and yang...
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Báo cáo khoa học: The Yin and Yang of protein folding doc
... identity of the polypeptide sequence [38,50]. The toxicity of different species and their rolein the development of disease is currently being explored for different protein systems [39]. The Yin and ... initiate the amyloidcascade. Especially in age-related amyloidosis, thismay lead to the accumulation of large quantities of partially folded proteins and the saturation of the capacity of the quality ... involved in amyloid disorders[27]. In the case of the enzyme lysozyme, the aggrega-tion of which is involved in hereditary systemic amy-loidosis, single point mutations in the lysozyme geneare...
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Báo cáo Y học: The processivity and fidelity of DNA synthesis exhibited by the reverse transcriptase of bovine leukemia virus pot
... activity of RT specifically hydrolyses the RNA in these heteroduplexes. Finally, the plus-senseDNA strand is synthesized by copying of the minus-senseDNA strand by the DNA-dependent DNA polymerase(DDDP) ... Virology 259, 176–189].In the p resent study, we have studied in vitro two features of the DNA polymerase activity of BLV RT, t he processivity of DNA synthesis and the fidelity of DNA synthesis. Theseproperties ... the well-studied RTs of human immunodeficiency virus type 1 (HIV-1) and murine leukaemia virus (MLV). Both the elongation of the DNA template and the processivity of DNA synthesisexhibited by...
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Báo cáo y học: "The use and abuse of commercial kits used to detect autoantibodies" ppsx
... high level of security, of scrutiny and of standardization, and under good labora-tory practices and quality control. Many of these compa-nies subscribe to and offer postmarketing qualityassurance ... achieve the goal of improving the quality of patient care through the use of autoantibodytesting and analysis. The matrix of manufacturing and application of autoantibody diagnostic kits (Fig. 1) The ... Analysis of the design of somestudies suggests that the lack of agreement between EIA and conventional assays may depend on the diagnosis and/ or the selection bias of the patients under study[2–4,6].A...
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Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx
... properly cited.AbstractJoint inflammation and destruction have been linked to the deregulation of the highly synthetic fibroblast-like synoviocytes(FLSs), and much of our current understanding of ... fusion protein and the reac-tivity and specificity of the purified IgY were confirmed usingGST-c19orf10 and a panel of unrelated His and GST fusionproteins. The antibody was then used to establish ... FLSs, there is an increase in the number of blood vessels, and the sub-intima becomes infil-trated with lymphocytes and plasma cells forming ectopic lym-phoid follicles [2]. The phenotype of the...
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Báo cáo y học: "The promise and reality of personal genomics" pot
... likely to be easy to both genotype and sequence. The sensitivity of SNP and indel detection in repetitive or copy-number-variable regions will be lower. Finally, a non-trivial proportion of any ... (Solexa) [4] and Applied Biosystem’s SOLiD [5] systems - but no explicit comparison of the two versions has yet been published.Minireview The promise and reality of personal genomicsBryndis Yngvadottir, ... not find comparisons of the two Korean genomes, or the two versions of the same Yoruba genome, for example. But the infant will grow, and the publication of AK1 and other individual genomes...
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Báo cáo y học: "omparative Efficacy and Tolerability of 5-Loxin® and Aflapin® Against Osteoarthritis of the Knee: A Double Blind, Randomized, Placebo Controlled Clinical Study"
... synthesized by chondrocytes and FLS. These cytokines act in various ways in the pathogenesis of OA such as inhibition o f s y nth esi s o f type 2 (articular) cartilage and activation of catabolic ... design of the project and data analysis, and was primarily responsible for writing the manuscript. KVA contributed to the de-sign of the project, patient recruitment and manage-ment, and data ... Raychaudhuri SP. A double blind, randomized, placebo controlled study of the efficacy and safety of 5-Loxin® for treatment of osteoarthritis of the knee. Arthritis Res. Ther. 2008; 10(4): R85 23....
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Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx
... membrane and in addition to de novosynthesis, the plant enzyme is capable of catalysing the exchange of the inositol polar head for another inositol. Ourdata clearly show that the CDP-diacylglycerol-independentexchange ... capacity of PtdIns synthase, especially if the membranes used as asource of enzyme are rich in PtdIns.Another question is the reason why CMP stimulates the exchange reaction and what the exact ... authors have identified the reverse products and shown that the CMP activation of the reverse of synthesis is by a different mechanism from base exchange[20]. Further analysis of the products released...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... regions adjacent to the beginning and the end of ybdQ and additional 20-nucleotidecomplementary to the template plasmid pKD13 carrying the kanamycin resistance (kan) gene. The resulted linear1.4-kb ... of the stationary phase the rate of synthesis of heat-shock proteins increases considerably,but only transiently [2,18]. Similarly, we observed that the steady-state amount of GroEL remains ... the deletion of the UP12 encoding gene increased the sensitivity of the mutant to CCCP (Fig. 6), it did not haveany detectable effects on the sensitivity to mitomycin C,unlike in the case of...
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Báo cáo Y học: The opgGIH and opgC genes of Rhodobacter sphaeroides form an operon that controls backbone synthesis and succinylation of osmoregulated periplasmic glucans pot
... coli,andwhatproperties they confer in this context. The highly anionic character of OPGs synthesized byE. coli is due to the presence of phosphoglycerol and succinyl residues. Two genes mdoB and ... govern the synthesis of OPGsbelonging to family I. In this paper, we describe the molecular and functional characterization of these genes,opgGIH, necessary for OPG backbone synthesis and of anew ... 70%ethanol from the cell pellets. The extracts were concentratedby rotary evaporation, and lipids and proteins were thenremoved by the addition of a mixture of chloroform and methanol (2 : 1). The aqueous...
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